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| 1 | Direct remyelinating effect of bone marrow mesenchymal stem cells in the rat model of spinal cord injury显示文摘Background:Several studies have tried to evaluate the effects of stem cell therapy for spinal cord injury in animal models and human trials.Bone marrow mesenchymal stem cells are one of the safe and available candidate cells with established beneficial therapeutic effects for bone and cartilage repair.However,there are some doubts regarding their therapeutic efficacy for nervous system disorders.Bone marrow mesenchymal stem cells have been used in some clinical trials for spinal cord injury but their precise mechanism of action and also their safety has not been investigated.Available experimental reports do not provide sufficient and convincing evidence to persuade therapists to perform further clinical trials.In the present study we have used detailed staining methods to clearly show the entire structure of the injured spinal cord and the effects of bone marrow mesenchymal stem cells treatment on this tissue.Methods:Intrathecal injection of neutral red stained bone marrow mesenchymal stem cells were done in a rat model of spinal cord injury and the fate of the transplanted cells,immunologic responses in the host tissue,and tumor generation were assessed by using brightfield and fluorescence microscopy.Sensory-motor functions were also evaluated in the animals.Results:Injected cells could migrate to the site of the injury and differentiate into oligodendrocytes and astrocytes.Immuno-histological assays showed no cells expressing CD2,CD3,CD15 and CD45 in host tissues,and sensory-motor assessments revealed that there were some partial improvements.Conclusion:Present study provides strong evidence that mesenchymal stem cell therapy is safe and could be helpful for the remyelination of injured nerve fibers in the acute phase of injury. | Farshad Homayouni Moghadam Abolghasem Abbasi Abbas Vakili-Zarch Mehrdad Mansouri Mojtaba Babaei-Zarch Mohammad Hossein Nasr-Esfahani | 2021 | Life Research2021，4，3： | 1 |
| 2 | Intrathoraeic kidney pres- ented with chest pain 显示文摘 | Abbas F Somaye R Farshad T | 2008 | IJKD: Iran J Kidney Dis2008，2，3： | 1 |
| 3 | Protective effect of Scrophularia striata combined with trehalose and cysteine added to diluents on cryopreservd goat epididymal sperm显示文摘Objective:To evaluate antioxidant effects of Scrophularia(S.)striata ethanol extract,trehalose and cysteine added to diluents on cryopreserved goat epididymal sperms.Methods:Motility and standard motion parameters of sperm were assessed by using computer assisted sperm motility analysis system.Sperm viability was evaluated by eosin-nigrosin staining method.Hypo-osmotic swelling test was used to evaluate membrane health.Thiobarbituric acid testing was used to measure malondialdehyde(MDA)concentrations.To assess DNA fragmentation,sperm chromatin dispersion test was used.In Experiment 1,treatments consisting of basal Tris diluent supplemented with 25,50 or 100µg/mL of S.striata ethanol extract gave the best concentration to the freezing diluents.Experiment 2 was carried out to compare the best concentration of S.striata ethanol extract(50µg/mL)resulting from the first experiment with 150 mM trehalose and/or 5 mM cysteine alone or in combination.Results:S.striata ethanol extract(50µg/mL)significantly increased sperm viability,motility and progressive motility and at the same time decreased MDA concentration and DNA fragmentation compared to other treatments(P<0.05).In addition,all treatment groups resulted in viability,membrane health,total motility,progressive motility,curvilinear velocity,straightline velocity higher and MDA lower compared to the control group(P<0.05).Acrosome integrity was significantly higher in 50µg/mL of S.striata ethanol extract combined with cysteine,trehalose,or cysteine+trehalose groups than those in the control,trehalose,cysteine,and 50µg/mL of S.striata ethanol extract groups(P<0.05).Regarding DNA,extenders supplemented with 50µg/mL of S.striata ethanol extract,50µg/mL of S.striata ethanol extract+trehalose,and 50µg/mL of S.striata ethanol extract+trehalose+cysteine were superior to other treatments.Conclusions:Adding 50µg/mL of S.striata ethanol extract alone or in combination with trehalose and cysteine can improve the quality of cryopreserved epididymal sperms of goats. | Zeinab Maleki Abbas Farshad Jalal Rostamzadeh | 2022 | Asian pacific Journal of Reproduction2022，11，2： | 0 |
| 4 | Effects of enzymatic and non-enzymatic antioxidants in diluents on cryopreserved bull epididymal sperm显示文摘Objective:To evaluate the supplementation effects of vitamin E,vitamin C,superoxide dismutase(SOD),and catalase to diluents on bull cryopreserved epididymal sperm.Methods:Sperm were retrieved from 20 bull testes and were then supplemented with 0.1 mM vitamin E,5.0 mM vitamin C,100.0 IU/mL SOD,and 100.0μg/mL catalase alone,or in a combination.The control treatment contained no addition.After supplementation,samples were frozen and stored in liquid nitrogen.The sperm parameters including motility,progressive motility,viability,acrosome integrity,plasma membrane integrity,kinematics and DNA damage were evaluated following the thawing process.Results:Vitamin E alone significantly increased the parameters of acrosome and membrane integrity compared to the control treatment(P<0.05).While compared to the control treatment,vitamin C had no improvement effect on sperm characteristics except for membrane integrity.Treatment of vitamin E+vitamin C had a significant improvement in total motility,progressive motility,viability,membrane and acrosome integrity compared to the control and other treatments(P<0.05).Compared to the control treatment,addition of SOD or catalase alone significantly improved the percentages of total motility,progressive motility,viability,membrane and acrosome integrity(P<0.05).Furthermore,SOD+catalase significantly increased total motility,progressive motility,viability,acrosome and membrane integrity characteristics compared to the catalase treatment(P<0.05).Vitamin E alone,vitamin E+vitamin C,and SOD in diluents decreased DNA damages and thereby improved the rate of intact sperm heads.Conclusions:Addition of 100.0 IU/mL SOD alone and 0.1 mM vitamin E+5.0 mM vitamin C,and also 5.0 mM vitamin C+100μg/mL catalase in a combination improves the quality of cryopreserved bull epididymal sperm and could be used for cryopreservation. | Hasan Sakhdary Abbas Farshad Jalal Rostamzadeh Fateme Bahri Binabaj Keyvan Sobhani | 2022 | Asian pacific Journal of Reproduction2022，11，1： | 0 |
| 5 | Prevalence of HIV infection and the correlates among homeless in Tehran,Iran显示文摘Objective:To determine the prevalence of HIV infection among homeless men and women and the related risk behaviors in Tehran,Iran.Methods:In 2007-2008,Tehran municipality stacked up 10657 homeless men and women for assessment of HIV and began collaboration with Iranian Research Center for HIV/AIDS(IRCHA)departments to conduct HIV infection prevalence surveys in homeless populations.The results were analyzed for associations with demographic information,family support,status of drug abuse and relation with family and friends.Results:Overall HIV prevalence was 1.7%(95%confidence interval 1.4-1.9).Factors independently associated with HIV infection included history of using drugs[AOR 8.15(4.86-13.67)].older age[AOR1.80(1.08-2.99)for 40-55 yr],occupation[AOR 1.64(1.19-2.24)for unemployed],and no relation with family[AOR 1.82(l.30-2.54)|.Conclusions:This study supports the idea that injection drug use is contributing to the increased spread of HIV among Iranian homeless.Harm reduction programs should be expanded,particularly among homeless injection drug users. | Abbas Ostad Taghi zadeh SeyedAhmad SeyedAlinaghi Farshad Fakhimi Hassanzad Mehdi Hajizadeh SeyedNajmeddin Mohamadi Sahra Emamzadeh-Fard Koosha Paydary Mostafa Hosseini | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，1： | 0 |
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