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| 1 | Differential regulation of protein synthesis in skeletal muscle and liver of neonatal pigs by leucine through an mTORC1-dependent pathway显示文摘Neonatal growth is characterized by a high protein synthesis rate that is largely due to an enhanced sensitivity to the postprandial rise in insulin and amino acids, especially leucine. The mechanism of leucine's action in vivo is not well understood. In this study, we investigated the effect of leucine infusion on protein synthesis in skeletal muscle and liver of neonatal pigs. To evaluate the mode of action of leucine, we used rapamycin, an inhibitor of mammalian target of rapamycin (mTOR) complex-1 (mTORC1). Overnight-fasted 7-day-old piglets were treated with rapamycin for 1 hour and then infused with leucine (400 μmol·kg -1 ·h -1 ) for 1 hour. Leucine infusion increased the rate of protein synthesis, and ribosomal protein S6 kinase 1 (S6K1) and eukaryotic initiation factor (eIF) 4E-binding protein-1 (4E-BP1) phosphorylation in gastrocnemius and masseter muscles (P < 0.05), but not in the liver. The leucine-induced stimulation of protein synthesis and S6K1 and 4E-BP1 phosphorylation were completely blocked by rapamycin, suggesting that leucine action is by an mTORC1-dependent mechanism. Neither leucine nor rapamycin had any effect on the activation of the upstream mTORC1 regulators, AMP-activated protein kinase and protein kinase B, in skeletal muscle or liver. The activation of eIF2a and elongation factor 2 was not affected by leucine or rapamycin, indicating that these two pathways are not limiting steps of leucine-induced protein synthesis. These results suggest that leucine stimulates muscle protein synthesis in neonatal pigs by inducing the activation of mTORC1 and its downstream pathway leading to mRNA translation. | Agus Suryawan Hanh V Nguyen Rosemarie D Almonaci Teresa A Davis | 2012 | Journal of Animal Science and Biotechnology2012，3，1： | 9 |
| 2 | Soil fertility in contour hedger- ow systems on sloping oxisols in Mindanao, Philippines显示文摘 | Agus F Garrity D P Cassel D K | 1999 | Soil and Tillage Research1999，50，： | 1 |
| 3 | Graduated compression stockings in the prevention of venous thrombo- embolism显示文摘 | AGU O SEIFALIAN A HAMILTON G BAKER D | 1999 | Br J Surg1999，86，： | 1 |
| 4 | Soil fertility in contour hedgerow systems on sloping oxisols in Mindanao Philippines显示文摘 | AGUS F GARRITY P CASSEL D K | 1999 | Soil and Tillage Research1999，50，2： | 1 |
| 5 | Tight glycemic control versus standard care after pediatric cardiac surgery显示文摘 | Agus MS Steil GM Wypij D | | 0，，： | 1 |
| 6 | Disinfection by-products and their potential impact on the quality of water produced by desalination systems:a literature review显示文摘 | Agus E Voutchkov N Sedlak D L | 2009 | Desalination2009，237，123： | 1 |
| 7 | Formation and fate of chlorination byproducts in reverse osmosis desalination systems显示文摘 | Agus E Sedlak D L | 2010 | Water Research2010，44，5： | 1 |
| 8 | Major antinutrients found in plant protein sources:Their effect on nutrition 显示文摘 | AKANDE K E DOMA U D AGU H O | 2010 | Pakistan Journal of Nutrition2010，9，8： | 1 |
| 9 | The management of horizontal- canal paroxysmal positional vertigo显示文摘 | Nuti D Agus G Barbieri MT | 1998 | Acta Otolaryngol1998，118，4： | 1 |
| 10 | Tight glycemic control versus standard care after pediatric cardiac surgery显示文摘 | Agus MSD Steil GM Wypij D | | 0，，13： | 1 |
| 11 | Effect of graduated compression stocking on limb oxygenation and venous function during exercise in patients with venous insufficiency显示文摘 | Agu O Baker D Seifalian AM | 2004 | Vascular2004，12，1： | 1 |
| 12 | Phase Ⅰ/Ⅱ study of bortezomib plus docetaxel in patients with advanced androgen-independent prostate cancer 显示文摘 | Dreicer R Petrylak D Agus D | 2007 | Clin Cancer Res2007，13，4： | 1 |
| 13 | Hydrogen production from glycerin by steam reforming over nickel catalysts显示文摘 | Adhikari Sushil Fernando Sandun D Haryanto Agus | 2008 | Renewable Energy2008，33，： | 1 |
| 14 | Graduated compression stockings in the prevention of venous thromboembolism 显示文摘 | Agu O Hamilton G Baker D | 1999 | Br J Surg1999，86，： | 1 |
| 15 | Proton-coupled oli- gopeptide transporter (POT) family expression in hu- man nasal epithelium and their drug transport potential 显示文摘 | Agu R Cowley E Shao D | 2011 | Mol Pharm2011，8，3： | 1 |
| 16 | Disinfection by-products and their potential impact on the quality of water produced by desalination systems: A literature review 显示文摘 | Agus E Voutchkov N Sedlak D L | 2009 | Desalination2009，237，13： | 1 |
| 17 | Proton - coupled oligopeptidetransporter (POT) family expression in human nasal epitheliumand their drug transport potential 显示文摘 | Agu R Cowley E Shao D | 2011 | Mol Pharma2011，8，3： | 1 |
| 18 | 14q32/miR- NA clusters loss of heterozygosity in acute lymphoblastic leukemia is associated with up-regulation of BCL1 la显示文摘 | AGUELI C CAMMARATA G SALEMI D | 2010 | Amer- ican Journal of Hematology2010，85，8： | 1 |
| 19 | Graduated compression stockings in the prevention of venous thromboembolism显示文摘 | Agu O Hamilton G Baker D | 1999 | Br J Surg1999，86，8： | 1 |
| 20 | Graduated compression stockings in the prevention of venous hromboembolism显示文摘 | Agu O Hamilton G Baker D | | 0，，： | 1 |
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