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| 1 | hsa-miR-29c and hsa-miR-135b differential expression aspotential biomarker of gastric carcinogenesis显示文摘AIM: To investigate the expression profiles of hsa-mi R-29 c and hsa-mi R-135 b in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. METHODS: The expression levels of hsa-mi R-29 c and hsa-mi R-135 b in normal gastric mucosa, non-atrophic chronic gastritis, intestinal metaplasia and intestinaltype gastric adenocarcinoma were analysed using quantitative real-time PCR. The difference between hsa-mi R-29 c and hsa-mi R-135 b expression profiles in the grouped samples was evaluated by ANOVA and Student's t-test tests. The results were adjusted for multiple testing by using Bonferroni's correction. P values ≤ 0.05 were considered statistically significant. To evaluate hsa-mi R-29 c and hsa-mi R-135 b expressions as potential biomarkers of gastric carcinogenesis, we performed a receiver operating characteristic curve analysis and the derived area under the curve, and a Categorical Principal Components Analysis. In silico identification of the genetic targets of hsa-mi R-29 c and hsa-mi R-135 b was performed using different prediction tools, in order to identify possible genes involved in gastric carcinogenesis.RESULTS: The expression levels of hsa-mi R-29 c were higher in normal gastric mucosal samples, and decreased progressively in non-atrophic chronic gastritis samples, intestinal metaplasia samples and intestinal-type gastric adenocarcinoma samples. The expression of hsa-mi R-29 c in the gastric lesions showed that non-atrophic gastritis have an intermediate profile to gastric normal mucosa and intestinal-type gastric adenocarcinoma, and that intestinal metaplasia samples presented an expression pattern similar to that in intestinal-type gastric adenocarcinoma. This micro RNA(mi RNA) has a good discriminatory accuracy between normal gastric samples and(1) intestinal-type gastric adenocarcinoma; and(2) intestinal metaplasia, and regulates the DMNT3 A oncogene. hsa-mi R-135 b is up-regulated in non-atrophic chronic gastritis and intestinal metaplasia samples and down-regulated in normal gastric mucosa and intestinal-type gastric adenocarcinoma samples. Non-atrophic chronic gastritis and intestinal metaplasia are significantly different from normal gastric mucosa samples. hsa-mi R-135 b expression presented a greater discriminatory accuracy between normal samples and gastric lesions. This mi RNA was associated with Helicobacter pylori presence in non-atrophic chronic gastritis samples and regulates the APC and KLF4 tumour suppressor genes.CONCLUSION: Our results provide evidence of epigenetic alterations in non-atrophic chronic gastritis and intestinal metaplasia and suggest that hsa-mi R-29 c and hsa-mi R-135 b are promising biomarkers of gastric carcinogenesis. | Amanda Ferreira Vidal Aline MP Cruz Leandro Magalhães Adenilson L Pereira Ana KM Anaissi Nélisson CF Alves Paulo JBS Albuquerque Rommel MR Burbano Samia Demachki Ândrea Ribeiro-dos-Santos | 2016 | World Journal of Gastroenterology2016，22，6： | 7 |
| 2 | Epidemiology of upper gastrointestinal symptoms in Brazil(Epi Gastro):A population-based study according to sex and age group显示文摘AIM:To determine the prevalences of symptoms consistent with gastroesophageal reflux disease(GERD)and dyspepsia in South America.METHODS:A telephone survey was conducted among adult owners of land-based telephones in S?o Paulo,Brazil,using previously validated computer-assisted sampling and survey protocols.The Portugueselanguage survey included(1)sociodemographic characteristics(e.g.,weight,height,smoking)and comorbidities;(2)dietary habits;(3)presence of symptoms consistent with GERD or dyspepsia within the prior 3mo;and(4)use of medications and other therapies to manage symptoms.Data were stratified post-hoc into three homogeneous geographical regions of S?o Paulo according to the Social Exclusion Indices of the districts and postal codes.Survey response data from each respondent were weighted by the numbers of adults and landline telephones in each household.The analyses were weighted to account for sampling design and to be representative of the S?o Paulo population according to city census data.RESULTS:Among 4570 households contacted,an adult from 3050(66.7%)agreed to participate.The nonresponse rate was 33.3%.The mean(SE)respondent age was 42.6(16.0)years.More than half of all respondents were women(53.1%),aged 18 through 49 years(66.7%),married or cohabitating(52.5%),and/or above normal-weight standards(i.e.,35.3%overweight and 16.3%obese).A total of 26.5%of women were perimenopausal.More than 20%of respondents reported highly frequent symptoms consistent with GERD(e.g.,gastric burning sensation=20.8%)or dyspepsia(e.g.,abdominal swelling/distension=20.9%)at least once per month.Prevalences of these symptoms were significantly(approximately 1.5-to 2.0-fold)higher among women than men but did not vary significantly as a function of advancing age.For instance,14.1%of women reported that they experienced stomach burning(symptom of GERD)at least twice per week,compared to 8.4%of men(P=0.012 byχ2 test).A total of 15.7%of women reported that they experienced abdominal swelling(symptom of dyspepsia)at least twice per week,compared to 6.4%of men(P<0.001byχ2 test).Despite frequent manifestations of GERD or dyspepsia,most(≥90%)respondents reported that they neither received prescription medications fromphysicians,nor took behavioral measures(e.g.,dietary modifications),to manage symptoms.CONCLUSION:Symptoms consistent with dyspepsia and GERD are prevalent in Brazil and represent major public-health and clinical challenges. | Maria do Rosário Dias de Oliveira Latorre Aline Medeiros da Silva Décio Chinzon Jaime N Eisig Telma RP Dias-Bastos | 2014 | World Journal of Gastroenterology2014，20，46： | 2 |
| 3 | Energetic-protein supplementation in the last 60 days of gestation improves performance of beef cows grazing tropical pastures显示文摘Background: Nutrition is one of the most important factors that affect animal performance, and it therefore also impacts on financial results in beef systems. In this way, finding the best strategy for feeding supplements is of paramount importance. Aiming to evaluate the effect of supplement feeding strategies for beef cows in the last third of gestation, two experiments were conducted. In Experiment 1, 35 pregnant Nellore cows were assigned to a completely randomized design with four treatments: control, which received no supplement; supplementation for the last 30 d of gestation(30-d; 3.0 kg/d); supplementation for the last 60 d of gestation(60-d; 1.5 kg/d); or supplementation for the last 90 d of gestation(90-d; 1.0 kg/d). All supplemented treatments received the same total amount of supplement throughout the experiment: 90 kg(20% of crude protein). A second experiment(Experiment 2) was delineated to evaluate the effects of the amounts offered in Experiment 1 on intake and metabolism. Four multiparous pregnant Nellore cows were assigned to a 4 × 4 Latin square design, with periods of15 d each.Results: There was a linear effect of the number of days of supplementation on calving body weight(BW; P < 0.05)and a quadratic effect on BW change from parturition to d 31 post-calving(P < 0.05), with cows on the 60-d strategy losing less BW post-calving. No difference was found in offspring birth BW(P > 0.10). A significant linear effect on interval from parturition to conception(P < 0.05) was observed, with the highest calving to conception interval being observed in the 90-d strategy. The level of supplementation did not affect forage intake or neutral detergent fiber digestibility(P > 0.10). Nitrogen excreted through urine tended to increase linearly with the level of supplementation(P < 0.10).Conclusion: Providing 1.5 kg of supplement during the last 60 d of gestation improves cow performance after calving, reducing the magnitude of BW lost, and reduces the number of days from calving to re-conception in the following breeding season compared to the usually recommended period of supplementation of 90 d pre-partum. | Aline Gomes da Silva Mário Fonseca Paulino Edenio Detmann Henrique Jorge Fernandes Lincoln da Silva Amorim Román Enrique Maza Ortega Victor Valério de Carvalho Josilaine Aparecida da Costa Lima Felipe Henrique de Moura Mariana Benevides Monteiro Jéssika Almeida Bitencourt | 2018 | Journal of Animal Science and Biotechnology2018，9，1： | 2 |
| 4 | Photoluminescence emissions of Ca_(1-x)WO_(4):xEu^(3+):Bridging between experiment and DFT calculations显示文摘In this work,the impact of the doping process on the photoluminescence emission of CaWO_(4) as a function of the concentration of Eu^(3+) cation(0.01 mol%,0.02 mol%,0.04 mol%,0.06 mol%,0.08 mol%,and 0.10 mol%) is discussed in detail.Ca_(1-x)WO~4:xEu^(3+) samples were successfully synthesized by a simple coprecipitation method followed by microwave irradiation.The blue shift in the absorption edge confirms the quantum confinement effect and the band gap energy covers the range from 3.91 to 4.18 eV,as the amount of Eu^(3+) cations increases.The experimental results are sustained by first-principles calculations,at the density functional theory level,to decipher the geometry and electronic properties,thereby enabling a more accurate and direct comparison between theory and experiment for the Ca_(1-x)WO_(4):xEu^(3+) structure. | Amanda Fernandes Gouveia Marcelo Assis Lara Kelly Ribeiro Aline Estefany Brandao Lima Eduardo de Oliveira Gomes Daniele Souza Yara Gobato Galvao Ieda Lucia Viana Rosa Geraldo Eduardo da Luz Jr Eva Guillamón Elson Longo Juan Andrés Miguel Angel San-Miguel | 2022 | Journal of Rare Earths2022，40，10： | 1 |
| 5 | Current capabilities of DES and LES for submarine at straight course显示文摘 | Alin N Bensow R E Fureby C | 2010 | Journal ofShip Research2010，54，3： | 1 |
| 6 | Removal of chromium from aqueous solution by complexation - ultrafiltration using a water- soluble macroligand显示文摘 | Aline A Bounatiro N Cherif A T | 2001 | Wat Res2001，35，9： | 1 |
| 7 | Prognostic impact of isoeitrate dehydrogenase enzyme isoforms 1 and 2 mutations in acute myeloid leukemia: a study by the Acute Leukemia French Association group 显示文摘 | Nicolas B Olivier N Aline R | 2010 | J Clin Oncol2010，28，23： | 1 |
| 8 | The ambivalence of challenge stres- sors :Time pressure associated with both negative and positive well- being 显示文摘 | Widmer P S Semmer N K K'alin W Jacobshagen N Meier L L | 2012 | Journal of Vocational Behav- ior2012，80，2： | 1 |
| 9 | Age - related changes in oxidized proteins 显示文摘 | Oliver C N Alin B W Moerman E J | 1987 | The Journal of Biological Chemistry1987，262，12： | 1 |
| 10 | A structural study of delafossite-type CuInO2 thin films 显示文摘 | Alan V Chadwich Aran N Blancklocks Aline Rougier | 2010 | J Phys: Conf Series2010，249，： | 1 |
| 11 | Video super resolution using generalized Gaussian markov random fields 显示文摘 | CHEN J JOSE N Y ALIN A | 2012 | IEEE Sig Proc Lett2012，19，2： | 1 |
| 12 | Prognostic Impact of Isocitrate Dehydrogenase Enzyme lsoforms 1 and 2 Muta- tions in Acute Myeloid Leukemia:A Study by the Acute Leukemia French Association Group 显示文摘 | Nieolas B Olivier N Aline R | 2010 | J Clin Oneol2010，28，23： | 1 |
| 13 | Age-related changes in oxidized proteins显示文摘 | Oliver C N Alin B W Moerman E J | 1987 | Journal of Biological Chemistry1987，262，： | 1 |
| 14 | A study on biological clogging of nonwoven geotextiles under leachate flow 显示文摘 | ENNION M P ALINE F N R MARIA L G R | 2008 | Geotextiles and Geomembranes2008，，26： | 1 |
| 15 | Age-related changes inoxidized proteins显示文摘 | Oliver C N Alin B W Moerman E J | 1987 | Journal of Biological Chemistry1987，262，12： | 1 |
| 16 | Age- related changes in oxidized proteins 显示文摘 | Oliver C N Alin B W Moerman E J | 1987 | Journal of Biological Chemistry1987，262，： | 1 |
| 17 | A study on biological clogging of nonwoven geotextiles under leachate flow显示文摘 | Ennio M Palmeiraa Aline F N Remigiob Maria L G Ramosc | 2008 | Geotextiles and Geomembranes2008，26，3： | 1 |
| 18 | Age-related Changes in oxidized Proteins 显示文摘 | OLIVER C N ALIN B W MOERMAN E J | 1987 | J Biol Chem1987，262，： | 1 |
| 19 | Melatonin prevents oxidative stress,inflammatory activity,and DNA damage in cirrhotic rats显示文摘BACKGROUND Cirrhosis is an important health problem characterized by a significant change in liver parenchyma.In animals,this can be reproduced by an experimental model of bile duct ligation(BDL).Melatonin(MLT)is a physiological hormone synthesized from serotonin that has been studied for its beneficial properties,including its antioxidant potential.AIM To evaluate MLT’s effects on oxidative stress,the inflammatory process,and DNA damage in an experimental model of secondary biliary cirrhosis.METHODS Male Wistar rats were divided into 4 groups:Control(CO),CO+MLT,BDL,and BDL+MLT.MLT was administered(20 mg/kg)daily beginning on day 15 after biliary obstruction.On day 29 the animals were killed.Blood samples,liver tissue,and bone marrow were collected for further analysis.RESULTS BDL caused changes in biochemical and histological parameters and markers of inflammatory process.Thiobarbituric acid(0.46±0.01)reactive substance levels,superoxide dismutase activity(2.30±0.07)and nitric oxide levels(2.48±0.36)were significantly lower(P<0.001)n the groups that received MLT.DNA damage was also lower(P<0.001)in MLT-treated groups(171.6±32.9)than the BDL-only group(295.5±34.8).Tissue damage and the expression of nuclear factor kappa B,interleukin-1β,Nrf2,NQO1 and Hsp70 were significantly lower in animals treated with MLT(P<0.001).CONCLUSION When administered to rats with BDL-induced secondary biliary cirrhosis,MLT effectively restored the evaluated parameters. | Josieli R Colares Renata M Hartmann Elizângela G Schemitt Sandielly R B Fonseca Marilda S Brasil Jaqueline N Picada Alexandre S Dias Aline F Bueno Cláudio A Marroni Norma P Marroni | 2022 | World Journal of Gastroenterology2022，28，3： | 1 |
| 20 | A study on biological clogging of nonwoven geotextiles under leachate flow 显示文摘 | Palmeira Ennio M Remigio Aline F N Ramos Maria L G | 2008 | Geotextiles and Geomemb:anes2008，26，3： | 1 |
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