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| 1 | Induction of deletion mutation on ompR gene of Salmonella enterica serovar Typhi isolates from asymptomatic typhoid carriers to evolve attenuated strains for vaccine development显示文摘Objective:To develop allenualed slrains of Salmonella enterica serorar Typhi(S.typhi) for the candidate vaccine by osmolar stress.Mothods:S.typhi SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Mamakkal,Tamil Nadu.India.Both strains were grown in LB(Luria Bertani) medium supplemented with various concentration of NaCl(0.1- 0.7M) respectively.The effecl of osmolar stress was determined at molecular level by PCR using MCR 06 and MCR07 primers corresponding to ompR with chromosomal DNA of S.typhi SS3 and SS5 strains.Attenuation by osmolar stress results in deletion mutation of the.S.typhi slrains was determined by agglutination assays,precipitation method.SDS PAGE analysis and by animal models.Results:The 799 bp amplified ompR gene product from wild type S.typhi SS3 and SS5 illustrate the presence of virulent gene.Interestingly,there was only a 282 bp amplified product from S.typhi SS3 and SS5 grown in the presence of 0.5.0.6 and 0.7 M NaCl.This illustrates the occurrence of deletion mutation in ompR gene al high concentration of NaCl.Furthermore,both the wildtype and mutant S.typhi outer membrane SDS-PAGF.profile reveals the differences in the expression of ompF.ompC and ompA proteins.In mice,wild type and mutant strains lethal dose(LD_(50)) were determined.The mice died within 72 h when both the wild type strains were injected intraperitoneally with 3 log CFU-mL^(-1).When the mice were injected with the mutants in same dosage,no clinical symptoms were observed;whereas the serum antibodv litre was elicited within two weeks indicated that the mutants have the ability to induce protective humoral immune response.These results suggest that S.typhi SS3 and SS5 may bo used as good candidate strains for the development of live attenuated vaccine against salmonellosis.Conclusions:This study demonstrates that the S.typhi strains were allenualed and could be good vaccine candidates in future. | Senthilkumar B Anbarasu K Senbagam D Rajasekarapandian M | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，12： | 2 |
| 2 | Effect of divalent cation substitution in the magnetoplumbite structured BaFe12O19 system 显示文摘 | Anbarasu V Md Gazzali P M Karthik T | 2013 | Journal of Materials Science-Materials in Electronics2013，42，4： | 1 |
| 3 | One-and-a-half ventricle repair for right ventricular endomyocardial fibrosis显示文摘 | Anbarasu M Krishna Manohar SR Titus T | 2004 | Asian Cardiovasc Thorac Ann2004，12，4： | 1 |
| 4 | Effects of ascorbic acid on the immune response of the catfish,Mystus gulio(Hamilton),to different bacterins of Ameromonas hydrophila显示文摘 | Anbarasu K Chandran M R | 2001 | Fish Shellfish Immunol2001，11，： | 1 |
| 5 | Effects of ascorbic acid on the immune response of the catfish, Mystus gulio (Hamilton), to different bacterins of Ameromonas hydrophila 显示文摘 | ANBARASU K CHANDRAN M R | 2001 | Fish & Shellfish Immunology2001，11，： | 1 |
| 6 | Effects of ascorbic acid on the immune response of the catfish,Mystus gulio (Hamilton),to different bacterins of Ameromonas hydrophila显示文摘 | Anbarasu K Chandran M R | 2001 | Fish Shellfish Immunol2001，11，： | 1 |
| 7 | Synthesis and characterization of polyethylene glycol (PEG) coated Fea O4 nanoparticles by chemical co-precipitation method for biomedical applications 显示文摘 | Anbarasu M Anandan M Chinnasamy E | 2015 | Spectrochimica Acta Part A:Molecular Biomolecular Spectroscopy2015，59，7： | 1 |
| 8 | The E3ubiquitin ligase Itch regulates tumor suppressor protein RASSF5/NORE1stability in an acetylation-dependent manner显示文摘 | Suryaraja R Anitha M Anbarasu K | 2013 | Cell Death Dis2013，4，： | 1 |
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