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| 1 | Expression and reconstitution of the bioluminescent Ca2+reporter aequorin in human embryonic stem cells,and exploration of the presence of functional IP3 and ryanodine receptors during the early stages of their differentiation into cardiomyocytes显示文摘In order to develop a novel method of visualizing possible Ca^(2+) signaling during the early differentiation of h ESCs into cardiomyocytes and avoid some of the inherent problems associated with using fluorescent reporters, we expressed the bioluminescent Ca^(2+) reporter, apo-aequorin, in HES2 cells and then reconstituted active holo-aequorin by incubation with f-coelenterazine. The temporal nature of the Ca^(2+) signals generated by the holo-f-aequorin-expressing HES2 cells during the earliest stages of differentiation into cardiomyocytes was then investigated. Our data show that no endogenous Ca^(2+) transients(generated by release from intracellular stores) were detected in 1–12-day-old cardiospheres but transients were generated in cardiospheres following stimulation with KCl or Ca Cl_2, indicating that holo-f-aequorin was functional in these cells. Furthermore, following the addition of exogenous ATP, an inositol trisphosphate receptor(IP_3R) agonist, small Ca^(2+) transients were generated from day 1 onward. That ATP was inducing Ca^(2+) release from functional IP_3 Rs was demonstrated by treatment with 2-APB, a known IP_3 R antagonist. In contrast, following treatment with caffeine, a ryanodine receptor(Ry R) agonist, a minimal Ca^(2+) response was observed at day 8 of differentiation only. Thus, our data indicate that unlike Ry Rs, IP_3 Rs are present and continually functional at these early stages of cardiomyocyte differentiation. | Harvey Y.S.Chan Man Chun Cheung Yi Gao Andrew L.Miller Sarah E.Webb | 2016 | Science China(Life Sciences)2016，59，8： | 5 |
| 2 | Serrated neoplasia of the colorectum显示文摘Serrated polyps of the colorectum form a group of related lesions which include aberrant crypt foci (ACF), conventional hyperplastic polyps, mixed (admixed) polyps, serrated adenomas and sessile serrated adenomas. In recent years the molecular differences between these morphologically similar lesions have been highlighted, and their differing biological potential has been realised. In particular, the sessile serrated adenoma has become recognised as the precursor lesion to a group of sporadic colorectal carcinomas characterised by morphological and molecular features distinct from conventional adenomas. These recent findings have challenged the long held paradigm that all colorectal carcinomas arise via the traditional adenoma-carcinoma sequence. In addition, they present a major challenge for the early detection and management of colorectal cancer, which is no longer regarded as a homogeneous entity. | Nathan T Harvey Andrew Ruszkiewicz | 2007 | World Journal of Gastroenterology2007，13，28： | 4 |
| 3 | Restrictions to the adaptation of influenza A virus H5 hemaggluti-nin to the human host显示文摘 | Andrew C R M Maria Z | 2004 | J Virol2004，78，： | 1 |
| 4 | Association of Contemporary Sensitive Troponin I Levels at Baseline and Change at 1 Year With Long-Term Coronary Events Following Myocardial Infarction or Unstable Angina显示文摘 | Harvey D. White Andrew Tonkin John Simes Ralph Stewart Kristy Mann Peter Thompson David Colquhoun Malcolm West Paul Nestel David Sullivan Anthony C. Keech David Hunt Stefan Blankenberg | 2014 | Journal of the American College of Cardiology2014，，4： | 1 |
| 5 | Enhancement of Cardiac Function and Suppression of Heart Failure Progression By Inhibition of Protein Phosphatase 1显示文摘 | Anand Pathak Federica del Monte Wen Zhao Jo-El Schultz John N. Lorenz Ilona Bodi Doug Weiser Harvey Hahn Andrew N. Carr Faisal Syed Nirmala Mavila Leena Jha Jiang Qian Yehia Marreez Guoli Chen Dennis W. McGraw E Kevin Heist J Luis Guerrero Anna A. DePaoli | 2005 | Circulation Research2005，，7： | 1 |
| 6 | A snapshot foveal hyper-spectral imager 显示文摘 | David William Fletcher-Holmes Andrew Robert Harvey | 2002 | SPIE2002，4816，： | 1 |
| 7 | Samarium — 153 EDTMP therapy of disseminated skeletal metastasis显示文摘 | J. Harvey Turner Andrew A. Martindale Peter Sorby Eric L. Hetherington Robert F. Fleay Ronald F. Hoffman Phillip G. Claringbold | 1989 | European Journal of Nuclear Medicine1989，，12： | 1 |
| 8 | Varied prevalence of Clostridium difficile in an integrated swine operation显示文摘 | K.N. Norman R.B. Harvey H.M. Scott M.E. Hume K. Andrews A.D. Brawley | 2009 | Anaerobe2009，，6： | 1 |
| 9 | IGF-I in epithelial ovarian cancer and its role in disease progression显示文摘 | Jane Brokaw Dionyssios Katsaros Andrew Wiley Lingeng Lu Dan Su Olga Sochirca Irene A. Rigault de la Longrais Susan Mayne Harvey Risch Herbert Yu | 2007 | Growth Factors2007，，5： | 1 |
| 10 | Management of spontaneous pneumothorax: British Thoracic Society pleural disease guideline 2010 显示文摘 | Andrew MacDuff Anthony Arnold John Harvey | 2010 | Thorax2010，65，2： | 1 |
| 11 | Forecasting, Structural Time Series Models and the Kalman Fiher 显示文摘 | Andrew Harvey | | International Journal of Fore- casting0，989，8： | 1 |
| 12 | Osteopontin is a novel downstream target of SOX9 with diagnostic implications for progression of liver fibrosis in humans显示文摘 | James Pritchett Emma Harvey Varinder Athwal Andrew Berry Cliff Rowe Fiona Oakley Anna Moles Derek A. Mann Nicoletta Bobola Andrew D. Sharrocks Brian J. Thomson Abed M. Zaitoun William L. Irving Indra N. Guha Neil A. Hanley Karen Piper Hanley | 2012 | Hepatology2012，，3： | 1 |
| 13 | Management of spontaneous pneumothorax : British Tho- racic Society pleural disease guideline 2010 显示文摘 | Andrew Mac Duff Anthony Arnold John Harvey | 2010 | Thorax2010，65，2： | 1 |
| 14 | Activity settings and travel behaviour: A social contact perspective显示文摘 | Andrew S. Harvey Maria Elena Taylor | 2000 | Transportation2000，，1： | 1 |
| 15 | A snapshot foveal hyper-spectral imager 显示文摘 | David William Fletcher-Holmes Andrew Robert Harvey | 2002 | SPIE2002，4816，： | 1 |
| 16 | LCP PCB- based Packaging for High-Performance Protection 显示文摘 | Linas Jauniskis Brian Farrell Andrew Harvey | 2006 | Advanced Packaging2006，，10： | 1 |
| 17 | The effect of capital structure when expected agency costs are extreme显示文摘 | Campbell R. Harvey Karl V. Lins Andrew H. Roper | 2004 | Journal of Financial Economics2004，，1： | 1 |
| 18 | Recombination between sequences of hepatitis B virus from different genotypes显示文摘 | Paul L. Bollyky Andrew Rambaut Paul H. Harvey Edward C. Holmes | 1996 | Journal of Molecular Evolution1996，，2： | 1 |
| 19 | Trend estimation and the hodrick prescott filter 显示文摘 | Andrew Harvey Thomas Trimbur | 2008 | Journal of Japan Statistic Society2008，38，1： | 1 |
| 20 | Determinants of rate variation in mammalian DNA sequence evolution显示文摘 | Lindell Bromham Andrew Rambaut Paul H. Harvey | 1996 | Journal of Molecular Evolution1996，，6： | 1 |
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