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| 1 | 异源nifA基因对苜蓿中华根瘤菌nifA突变体的互补分析显示文摘为深入研究苜蓿中华根瘤菌NifA的特性,分别用组成型表达的慢生型大豆根瘤菌和紫云英根瘤菌的nifA基因互补苜蓿中华根瘤菌nifA突变体,观察其共生表型.结果表明,慢生型大豆根瘤菌和紫云英根瘤菌nifA基因不能互补苜蓿中华根瘤菌nifA突变体的共生表型.以苜蓿中华根瘤菌nifA突变体为遗传背景,利用全基因组微阵列实验比较分析引入异源nifA基因后产生的基因表达谱的变化.结果显示,苜蓿中华根瘤菌自身NifA蛋白引起238个基因的表达发生变化.这些表达差异的基因分属共生、能量和中心代谢、持家、细胞结构与运输等生物学功能组.慢生型大豆根瘤菌、紫云英根瘤菌和阴沟肠杆菌的NifA蛋白分别引起了20,7和9个基因的表达发生变化.这些基因主要是固氮相关基因,但差异不及苜蓿中华根瘤菌NifA互补菌明显.以苜蓿中华根瘤菌nifH启动子与lacZ融合基因为报道基因,研究nifH的表达.结果指出,慢生型大豆根瘤菌和紫云英根瘤菌的NifA蛋白只能部分激活苜蓿中华根瘤菌nifH的表达,激活水平分别为苜蓿中华根瘤菌NifA蛋白激活率的70%和50%,与微阵列实验结果相符. | 姚振华 田哲贤 戴小密 Anke Becker 李健 严海芹 肖琰 朱家璧 俞冠翘 Silvia Rüberg 王忆平 邹华松 | 2006 | 科学通报2006，51，19： | 4 |
| 2 | nifA突变的苜蓿根瘤菌在根瘤中的转录组学分析显示文摘用全基因组微阵列比较了根瘤中苜蓿根瘤菌1021的nifA突变菌和野生菌的基因表达谱.分析结果表明,nifA的缺失引起601个基因的表达发生变化.这些基因分别属于生物大分子的合成代谢、三羧酸循环及呼吸代谢以及结瘤固氮过程等多个功能组,预示着根瘤中细菌的生长状态发生了显著的变化.在根瘤中,fixK以及受其激活的基因在nifA突变菌中的表达量显著高于野生菌.定量实时PCR分析表明,根瘤中fixLJ的转录水平在nifA突变菌中显著高于野生菌.通过统计学方法从13个表达发生显著变化的基因的上游调控序列中找到推测的NifA结合位点. | 田哲贤 邹华松 李健 张远涛 刘影 俞冠翘 朱家璧 Silvia Rüberg Anke Becker 王忆平 | 2006 | 科学通报2006，51，15： | 3 |
| 3 | Multivariate statistics as a tool for modelbased prediction of floodplain vegetation and fanna显示文摘 | ANKE H STEPHAN R | 2003 | Ecological Modelling2003，169，： | 1 |
| 4 | Hemodynamic assessment of acute stroke using dynamic single-slice computed tomographic perfusion imaging显示文摘 | Joachim R Lars J Anke F | 2000 | Arch Neurol2000，57，： | 1 |
| 5 | Anti -CD20 - and B -cell re- ceptor - mediated apoptosis : evidence for shared intracellular signaling pathways 显示文摘 | Stephan M Anke R Kurt BB | 2000 | Cancer Research2000，60，24： | 1 |
| 6 | Zoonotic Chlamydiaceae species associated with Trachoma,Nepal显示文摘 | Deborah D James R Anke R | 2013 | Emerg Infect Dis2013，19，12： | 1 |
| 7 | Fit-1 signaling in mac rophages promotes glioma growth in vivo显示文摘 | Mark K Yvonne R Anke W | 2008 | Cancer Res2008，68，18： | 1 |
| 8 | Ternperature effects on the radiation response of MOS devices显示文摘 | Schw ank J R Sexton F W Fleetwood D M | 1988 | IEEE T rans N ucl Sci1988，35，6： | 1 |
| 9 | Multivariate statistics as a tool for model-based prediction of floodplain vegetation and fauna显示文摘 | ANKE H STEPHAN R | 2003 | Ecological Modelling2003，169，： | 1 |
| 10 | Flt-I signaling in macrophages promotes glionm growth in vivo 显示文摘 | Mark K Yvonne R Anke W | 2008 | Cancer Res2008，68，18： | 1 |
| 11 | AntiCandida metabolites from endophytic fungi显示文摘 | Weber R W Kappe R Paululat T M(o)sker E Anke H | 2007 | Phytochemistry2007，68，： | 1 |
| 12 | Effects of irradiation temperature on MOS radiation response显示文摘 | Shaneyfelt M R Schw ank J R Fleetwood D M | 1998 | IEEE T rans N ud Sci1998，45，3： | 1 |
| 13 | Detelopment of a research strategy for assessing the ecological risk of endorcrine distuptors显示文摘 | Ankely G T Jonnson R D Detenbeck N E | 1997 | Rev Toxicol1997，18，3： | 1 |
| 14 | Age-Related Changes in Central and Peripheral Corneal Thickness: Determination of Normal Values With the Orbscan II Topography System显示文摘 | Florian Rüfer Anke Schr?der Christine Bader Carl Erb | 2007 | Cornea2007，，1： | 1 |
| 15 | Multivariate statistics as a tool for model-based prediction of floodplain vegetation and fauna显示文摘 | Anke H Stephan R | 2003 | Ecological Modelling2003，169，： | 1 |
| 16 | White-to-White Corneal Diameter: Normal Values in Healthy Humans Obtained With the Orbscan II Topography System显示文摘 | Florian Rüfer Anke Schr?der Carl Erb | 2005 | Cornea2005，，3： | 1 |
| 17 | Effect of interleukin (IL) - 12 and IL--15 on activated natural killer(ANK) and antibody -de- pendent cellular cytotoxicity ( ADCC ) in HIV infection 显示文摘 | Lin S J Roberts R L Ank B J | 1998 | J Clin lmmunol1998，18，5： | 1 |
| 18 | Oxygen free radical mediated injury tocultured rat hepatocytes during cold incubation in preservation solution显示文摘 | Ursula R Irith R Anke F | 1997 | Hepatology1997，26，： | 1 |
| 19 | Oxygen free radical mediated injury to cultured rat hepatocytes during cold incubation in preservation solution显示文摘 | Irith R Anke F | 1997 | Hepatology1997，26，： | 1 |
| 20 | Neural network based iterative learning predictive control design for mechatronic systems with isolated nonlinearity显示文摘 | Zhang R Xue Anke Wang Jian-zhong | 2009 | Journal of Process Control2009，19，1： | 1 |
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