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| 1 | Increased ATG5-ATG12 in hepatitis B virus-associated hepatocellular carcinoma and their role in apoptosis显示文摘AIM To investigate autophagy-related genes, particularly ATG12, in apoptosis and cell cycle in hepatitis B virus(HBV)-associated hepatocellular carcinoma(HCC) and non-HBV-HCC cell lines.METHODS The expression of autophagy-related genes in HBVassociated hepatocellular carcinoma and non-HBV-HCC cell lines and human liver tissues was examined by quantitative real-time reverse transcriptase-polymerase chain reaction(q RT-PCR) and western blotting. The silencing of target genes was used to examine the function of various genes in apoptosis and cell cycle progression. RESULTS The expression of autophagy related genes ATG5, ATG12, ATG9 A and ATG4 B expression was analyzed in Hep G2.2.15 cells and compared with Hep G2 and THLE cells. We found that ATG5 and ATG12 m RNA expression was significantly increased in Hep G2.2.15 cells compared to HepG 2 cells(P < 0.005). Moreover, ATG5-ATG12 protein levels were increased in tumor liver tissues compared to adjacent non-tumor tissues mainly from HCC patients with HBV infection. We also analyzed the function of ATG12 in cell apoptosis and cell cycle progression. The percentage of apoptotic cells increased by 11.4% in ATG12-silenced Hep G2.2.15 cells(P < 0.005) but did not change in ATG12-silenced HepG 2 cells under starvation with Earle's balanced salt solution. However, the combination blockade of Notch signaling and ATG12 decreased the apoptotic rate of HepG 2.2.15 cells from 55.6% to 50.4%(P < 0.05). CONCLUSION ATG12 is important for HBV-associated apoptosis and a potential drug target for HBV-HCC. Combination inhibition of ATG12/Notch signaling had no additional effect on HepG 2.2.15 apoptosis. | Areerat Kunanopparat Ingorn Kimkong Tanapat Palaga Pisit Tangkijvanich Boonchoo Sirichindakul Nattiya Hirankarn | 2016 | World Journal of Gastroenterology2016，22，37： | 11 |
| 2 | Delta-like ligand 4 in hepatocellular carcinoma intrinsically promotes tumour growth and suppresses hepatitis B virus replication显示文摘AIM To investigate the role of Delta-like ligand 4(DLL4) on tumour growth in hepatitis B virus(HBV)-associated hepatocellular carcinoma(HCC) in vivo.METHODS We suppressed DLL4 expression in an HBV expressing HCC cell line, HepG2.2.15 and analysed the growth ability of cells as subcutaneous tumours in nude mice. The expression of tumour angiogenesis regulators, VEGF-A and VEGF-R2 in tumour xenografts were examined by western blotting. The tumour proliferation and neovasculature were examined by immunohistochemistry. The viral replication and viral protein expression were measured by quantitative PCR and western blotting, respectively.RESULTS Eighteen days after implantation, tumour volume in mice implanted with sh DLL4 HepG2.2.15 was significantly smaller than in mice implanted with control HepG2.2.15(P < 0.0001). The levels of angiogenesis regulators, VEGF-A and VEGF-R2 were significantly decreased in implanted tumours with suppressed DLL4 compared with the control group(P < 0.001 and P < 0.05, respectively). Furthermore, the suppression of DLL4 expression in tumour cells reduced cell proliferation and the formation of new blood vessels in tumours. Unexpectedly, increased viral replication was observed after suppression of DLL4 in the tumours.CONCLUSION This study demonstrates that DLL4 is important in regulating the tumour growth of HBV-associated HCC as well as the neovascularization and suppression of HBV replication. | Areerat Kunanopparat Jiraphorn Issara-Amphorn Asada Leelahavanichkul Anapat Sanpavat Suthiluk Patumraj Pisit Tangkijvanich Tanapat Palaga Nattiya Hirankarn | 2018 | World Journal of Gastroenterology2018，24，34： | 3 |
| 3 | Measurement And Prediction of LDPE/CO2 Solution Viscosity显示文摘 | Areerat Surat Nagata Takefumi Ohshima Masahiro | 2002 | Polymer Engineering and Science2002，42，11： | 1 |
| 4 | Measurement and prediction of LDPE/CO: solution viscosity显示文摘 | Surat Areerat Takefumi Nagata Masahiro Ohshim | 2002 | Polymer Engineering and Science2002，42，11： | 1 |
| 5 | The effects of processing parameters on the residual wall thickness distribution at the sharp angle comer of water assisted injection molded parts 显示文摘 | PUDPONG T BUAHOM P AREERAT S | 2013 | Int Polym Proc2013，28，5： | 1 |
| 6 | Constituents of Polyalthia jucunda . and Their Cytotoxic Effect on Human Cancer Cell Lines显示文摘 | Areerat Suedee Ing-On Mondranondra Anake Kijjoa Madalena Pinto Nair Nazareth Maria S?o José Nascimento Artur M.S. Silva Werner Herz | 2007 | Pharmaceutical Biology2007，，7： | 1 |
| 7 | Analysis of specific IgM responses in secondary dengue virus infections: levels and positive rates in comparison with primary infections显示文摘 | Sumalee Chanama Surapee Anantapreecha Atchareeya A-nuegoonpipat Areerat Sa-gnasang Ichiro Kurane Pathom Sawanpanyalert | 2004 | Journal of Clinical Virology2004，，3： | 1 |
| 8 | Solubility of carbon dioxide in polyethylene/titanium dioxide composite under high pressure and temperature显示文摘 | Areerat S Hayata Y Katsumoto R | 2002 | J Appl Polym Sci2002，86，2： | 1 |
| 9 | Measurement and solution viscosity显示文摘 | S Areerat T Nagata M prediction of LDPE/CO2 Ohshima | 2002 | Polymer Engineering and Science2002，42，11： | 1 |
| 10 | Measurement and prediction of diffusion coefficients of supercritical CO2 in molten polymers显示文摘 | S Areerat E Funami Y Hayata | 2004 | Polymer Engineering and Science2004，44，10： | 1 |
| 11 | Measurement and Prediction of LDPE/CO2 Solution Viscosity显示文摘 | S Areerat T Nagata M Ohshima | 2002 | Polymer Engineering & Science2002，42，11： | 1 |
| 12 | Measurement and Prediction of LDPE/CO2 Solution Viscosity 显示文摘 | Surat Areerat Takefumi Nagata Masahiro Ohshima | 2002 | Poly Eng Sci2002，42，11： | 1 |
| 13 | Cross-reactive IgM responses in patients with dengue or Japanese encephalitis显示文摘 | Atchareeya A-Nuegoonpipat Nirandorn Panthuyosri Surapee Anantapreecha Sumalee Chanama Areerat Sa-Ngasang Pathom Sawanpanyalert Ichiro Kurane | 2007 | Journal of Clinical Virology2007，，1： | 1 |
| 14 | Virus isolation from epizootic ulcerative syndrome-diseased fishes 显示文摘 | Kanchanakhan S Saduakdee U Areerat S | 1999 | Asian Fisheries Science1999，12，： | 1 |
| 15 | The effects of processing parameters on the residual wall thickness distribution at the sharp angle comer of water as- sisted injection molded parts 显示文摘 | PUDPONG T BUAHOM P AREERAT S | 2013 | Int Polym Proc2013，28，5： | 1 |
| 16 | Measurement and prediction of diffusion coefficients of supercritical CO2 in molten polymers 显示文摘 | Areerat S Funami E Hayata Y | 2004 | Poly Eng Sci2004，44，10： | 1 |
| 17 | Comparison between haemagglutination inhibition (HI) test and IgM and IgGcapture ELISA in determination of primary and secondary dengue virus infections显示文摘 | Atchareeya A Songthum P Areerat S | 2006 | Dengue Bulletin2006，30，14： | 1 |
| 18 | Trans PacificStategic Economic Partnership With Japan, South Korea andChina Integrate: General Equilibrium Approach 显示文摘 | Areerat T Kameyama H Ito S | 2012 | AmericanJournal of Economics and Business Administration2012，，1： | 1 |
| 19 | Trans Pacific Strategic Economic partnership With Ja- pan, South Korea and China Integrate: General Equilibrium Approach 显示文摘 | Todsadee Areerat Hiroshi Kameyama Shoichi Ito and Koh - en Yamauchi | 2012 | American Journal of Economics and Business Admin- istration2012，4，1： | 1 |
| 20 | Solubility of carbon dioxide inpolyethylene/titanium dioxide composite under high pressureand temperature 显示文摘 | Areerat S Hayata Y KatsumotoR KegasawaT EgamiH Ohshima M | 2002 | Journal of Applied PolymerScience2002，86，2： | 1 |
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