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| 1 | IL28B polymorphism and cytomegalovirus predict response to treatment in Egyptian HCV type 4 patients显示文摘AIM:To test whether the status of positive cytomegalovirus(CMV) DNA detection adds to the predictive value of IL28B and to further categorize C/T allele carriers.METHODS:This study included 166 chronic hepatitis C(CHC) patients who received combined interferon and ribavirin therapy for 48 wk,84 spontaneous hepatitis C virus(HCV) resolvers who were positive for IgG anti-HCV antibody and negative for HCV RNA,and 100 healthy subjects who were negative for both HCV antibodies and RNA as controls.Genomic DNA from peripheral blood was used for IL28B rs.12979860 single nucleotide polymorphism(SNP) and CMV DNA detection.A 139 bp fragment containing IL28B SNP was amplified in all subjects by polymerase chain reaction using a specifically designed primer.Then the IL28B rs.12979860 SNP was detected by restriction fragment length polymorphism(RFLP) genotyping.The presence of CMV DNA was tested by amplification of the gB1 gene using nested polymerase chain reaction.The role of CMV and IL28B rs.12979860 SNP genotypes in determining the response rate to combined interferon therapy and clinical status of patients were statistically analyzed.RESULTS:Current data showed that 67% of patients carrying the IL28B 12979860 C/C allele had a sustained viral response(SVR) while the genotypes C/T and TT were associated with lower SVR rates,50% and 48%,respectively.SVR rates for the C/C allele were lower than other HCV genotypes and/or other populations.Genotype CC was associated with the response to interferon(P = 0.025).Genotype C/C was reduced from 48% in controls to 14% in CHC patients suggesting its protective role against progression to chronicity.The majority of spontaneously cleared subjects(86%) were C/C,confirming its protective role.The C/T allele was present in 71% of CHC patients compared with 38% of controls,so the use of IL28B SNP genotyping only in these patients may be of little value as a predictor of response.CMV reactivation occurred in 40% of CHC patients.Co-infection with CMV seriously diminished the response to interferon(IFN) therapy,with SVR rates in C/C genotypes 87.5% in CMV-negative patients and 12.5% in CMV-positive patients(P < 0.0001).SVR rates among C/T carriers were reduced to < 50% in patients with positive CMV DNA while the non-response rate doubled.These data indicate that a supplemental assay for CMV viremia adds to the prognostic value of IL28B genotyping.CONCLUSION:The results suggest that both genetic(i.e.,spontaneous) and therapeutic(IFN-based therapy) arms are complementary in the battle against HCV.CMV DNA testing may be of value to better predict the response to IFN,particularly in IL28B C/T carriers. | Mostafa K El Awady Noha G Bader El Din Ashraf Tabll Yaser El Hosary Ashraf O Abdel Aziz Hesham El Khayat Mohsen Salama Tawfeek H Abdelhafez | 2013 | World Journal of Gastroenterology2013，19，2： | 8 |
| 2 | Skeletal muscle mitochondrial health and spinal cord injury显示文摘Mitochondria are the main source of cellular energy production and are dynamic organelles that undergo biogenesis, remodeling, and degradation. Mitochondrial dysfunction is observed in a number of disease states including acute and chronic central or peripheral nervous system injury by traumatic brain injury, spinal cord injury(SCI), and neurodegenerative disease as well as in metabolic disturbances such as insulin resistance, type Ⅱ diabetes and obesity. Mitochondrial dysfunction is most commonly observed in high energy requiring tissues like the brain and skeletal muscle. In persons with chronic SCI, changes to skeletal muscle may include remarkable atrophy and conversion of muscle fiber type from oxidative to fast glycolytic, combined with increased infiltration of intramuscular adipose tissue. These changes contribute to a proinflammatory environment, glucose intolerance and insulin resistance. The loss of metabolically active muscle combined with inactivity predisposes individuals with SCI to type Ⅱ diabetes and obesity. The contribution of skeletal muscle mitochondrial density and electron transport chain activity to the development of the aforementioned comorbidities following SCI is unclear. A better understanding of the mechanisms involved in skeletal muscle mitochondrial dynamics is imperative to designing and testing effective treatments for this growing population. The current editorial will review ways to study mitochondrial function and the importance of improving skeletal muscle mitochondrial health in clinical populations with a special focus on chronic SCI. | Laura C O'Brien Ashraf S Gorgey | 2016 | World Journal of Orthopedics2016，7，10： | 3 |
| 3 | Finite Element Model Updating Approach to Damage Identification in Beams Using Par- ticle Swarm Optimization 显示文摘 | Mohamed M S Mustafa H A Ashraf O | 2013 | Engineering Optimization2013，45，6： | 1 |
| 4 | Holmium: YAG lasertripsy for ureteric calculi : an experience of 300 procedures显示文摘 | Devarajan R Ashraf M Beck R O | 1998 | Br J Urol1998，82，3： | 1 |
| 5 | Redo coronary artery bypass grafting:role of arterial grafts and time interval显示文摘 | Ashraf O | 2006 | J Thorac Cardiovasc Surg2006，132，1： | 1 |
| 6 | Holmium:YAG Lasertripsy for ureteric colculi:an experience of 300 procedures显示文摘 | DEVARAJAN R ASHRAF M BECK R O | 1998 | Br J Urol1998，82，： | 1 |
| 7 | Distribution of cations in leaves of salt -tolerant and salt - sensitive lines of sunflower under saline conditions 显示文摘 | Ashraf M O' Leary J W | 1995 | Journal of Plant Nutrition1995，18，： | 1 |
| 8 | A particle swarm- based genetic algorithm for scheduling in an agile environment 显示文摘 | LOTFI K G SHERIFA M ASHRAF O N | 2008 | Computers & Industrial Engineering2008，55，3： | 1 |
| 9 | Does pattern of ion accumulation vary in alfalfa at different growth stages显示文摘 | O'Leary JW | 1994 | J Plant Nutr1994，17，： | 1 |
| 10 | Role of hysteroseopy in diagnosis of subtle uterine anomalies in patients with normal hysterosalpingography 显示文摘 | Kalia N Abuzeid O Ashraf M | 2011 | Fertility and Sterility2011，96，3： | 1 |
| 11 | Trends of diabetes according to body mass index levels in Dan: re- sults of the national surveys of risk factors of non-com- municable diseases ( 1999-2007 )显示文摘 | Esteghamati A Ashraf H Khalilzadeh O | 2010 | Diabetic Med2010，27，11： | 1 |
| 12 | The role of matrix metalloproteinases in ulcerative keratolysis associated with perioperative diclofenac use显示文摘 | O'Brien TP Li QJ Sauerburger F Reviglio VE Rana T Ashraf MF | 2001 | Ophthalmology2001，108，4： | 1 |
| 13 | Ground movement predictions for braced excavations in undrained day显示文摘 | Ashraf S O Malcolm D B | 2006 | Journal of geotechnical and geoenvironmental engineering2006，，4： | 1 |
| 14 | Holmium: YAG lasertripsy for ureteric calculi: an experience of 300 procedures显示文摘 | DEVARAJAN R ASHRAF M BECK R O | 1998 | Br J Urol1998，82，3： | 1 |
| 15 | Dose pattern of ion accumulation vary in alfalfa at different growth stages显示文摘 | Ashraf M O'Leary J W | 1994 | J Plant Nutr1994，17，： | 1 |
| 16 | Holmium: YAG lasertripsy for ureferic calculi: an experience of 300 procedures 显示文摘 | Devarajian R Ashraf M Beck R O | 1998 | Br J Urol1998，82，： | 1 |
| 17 | Effect of hypoxia on the expression of pro-and anti-apoptotic proteins in neuronal nuclei of the guinea pig fetus during gestation显示文摘 | ABEDIN N ASHRAF Q MISHA O P | 2005 | Barin Res Dev2005，156，1： | 1 |
| 18 | Hypoxia-induced Bax and Bel-2 protein expression ,caspase-9 activation, DNA fragmentation,and lipid peroxidation in mitochondria of the cerebral cortex of newborn piglets:the role of nitric oxide显示文摘 | Mishra O P Randis T Ashraf Q M | 2006 | Neuroscience2006，141，3： | 1 |
| 19 | The role of matrix metalloproteinases in ulcerative keratolysis associated with perioperative diclofenac use显示文摘 | O'Brien TP Li QJ Sauerburger F Reviglio VE Rana T Ashraf MF | 2001 | Ophthalmology2001，108，： | 1 |
| 20 | Transit time flow measurement in on-pump and off-pump coronary artery surgery 显示文摘 | Schmitz C Ashraf O Schiller W | 2003 | J Thorac Cardiovasc Surg2003，126，3： | 1 |
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