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| 1 | Effect of adipose-derived mesenchymal stem cells on hepatocellular carcinoma: In vitro inhibition of carcinogenesis显示文摘AIM To investigate the effect of adipose-derived mesenchymal stem cells(ADMSCs)and their conditioned media(CM) on hepatocellular carcinoma(HCC) cell tumorigenesis.METHODS The proliferation rate of HepG2 and PLC-PRF-5 HCC cancer cells was measured using the trypan blue exclusion method and confirmed using the cell-counting kit8(commonly known as CCK-8) assay. Apoptosis was detected by flow cytometry using annexin V-FITC. Protein and mRNA expression was quantified by ELISA and real time PCR, respectively. Migration and invasion rates were performed by Transwell migration and invasion assays. Wound healing was examined to confirm the data obtained from the migration assays.RESULTS Our data demonstrated that when co-culturing HCC cell lines with ADMSCs or treating them with ADMSC CM, the HCC cell proliferation rate was significantly inhibited and the apoptosis rate increased. The decreased proliferation rate was accompanied by an upregulation of P53 and Retinoblastoma mRNA and a downregulation of c-Myc and hTERT mRNA levels. More notably, ADMSCs and their CM suppressed the expression of the two important markers of HCC carcinogenicity, alpha-fetoprotein and Des-gamma-carboxyprothrombin. In addition, the migration and invasion levels of HepG2 and PLC-PRF-5 cells significantly decreased, potentially through increased expression of the tissue inhibitor metalloproteinases TIMP-1, TIMP-2 and TIMP-3.CONCLUSION These findings shed new light on a protective and therapeutic role for ADMSCs and their CM in controlling HCC invasiveness and carcinogenesis. | Rim Serhal Nagib Saliba George Hilal Mayssam Moussa Ghada S Hassan Oula El Atat Nada Alaaeddine | 2019 | World Journal of Gastroenterology2019，25，5： | 6 |
| 2 | Sudden cardiac death in patients with diabetes 显示文摘 | EL ATAT F A MCFARLANE S I SOWERS J R | 2004 | Curr Diab Rep2004，4，3： | 1 |
| 3 | Spectroscopic ( NMR, UV, FT-IR and FT-Raman)analysis and theoreticalinvestigation of nicotinamide N-oxide with density functional theory 显示文摘 | ATAT A KARABACAK M KOSE E | 2011 | Spectrochimica Acta Part A : Molecular and Biomolecular Spectroscopy2011，，83： | 1 |
| 4 | Inherited protein variation and parentage testing in farmed red deer显示文摘 | ATate M L | 1990 | Anim Breed Absts1990，58，9： | 1 |
| 5 | The relationship between hyperinsulinemia,hypertension and progressive renal disease显示文摘 | Atat FA Stas SN McFarlane SI | | 0，，： | 1 |
| 6 | The relationship between hyperinsulinemia, hypertension and progressive renal disease 显示文摘 | El - Atat FA Stas SN McFarlane SI | 2004 | J Am Soc Nephrol2004，15，11： | 1 |
| 7 | Clinical presentation and outcome of bladder schistosoma-unrelated squamous cell carcinoma : report on 33 consecutive cases 显示文摘 | Riadh BS E1 Atat R Sfaxi M | 2007 | ClinGenitourin Cancer2007，5，6： | 1 |
| 8 | Noninvasive transcutaneous low frepuency ultrasound enhances thrombolysis in perpheral and coronary arteries 显示文摘 | Atat S Fishbein MC | 2001 | Echoardiography2001，18，3： | 1 |
| 9 | Quantum-Dot Spin-State Preparation with Near-Unity Fidelity显示文摘 | METE ATAT(U)RE JAN DREISER | 2006 | Science2006，312，： | 1 |
| 10 | The relationship between hyperinsulinemia, hypertension and progressive renaldisease 显示文摘 | EI Atat FA Stas SN Mcfarlane SI | 2004 | SoeNephro2004，15，11： | 1 |
| 11 | The relationship between hyperinsulinemia, hypertension and progressive renal disease显示文摘 | EL ATAT F A STAS S N MCFARLANE S I | 2004 | J Am Soc Nephrol2004，15，11： | 1 |
| 12 | Retroperitoneal cystic lym- phangiomas in adults: four case reports and review of the litera- ture 显示文摘 | TOHMEC ATAT GHORRAC | 2008 | JMed Liban2008，56，1： | 1 |
| 13 | The relationship between hyperinsulinemia,hypertension and progressive renal disease显示文摘 | EL ATAT F A STAS S N MCFARLANE S I | 2004 | Soc Nephrol2004，15，11： | 1 |
| 14 | Relativistic Treatment of Massive Klein-Gordon Particle by Modified Generalized Hulthen Potential显示文摘 | Akaninyene D. Antia Imeh E. Essien Joseph G. Atat | 2017 | Journal of Mathematics and System Science2017，7，2： | 0 |
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