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| 1 | Isolation and diagnosis of Helicobacter pylori by a new method: Microcapillary culture显示文摘AIM:To investigate the performance of the microcapillary culture method(MCM) in Helicobacter pylori(H.pylori) isolation and diagnosis.METHODS:Microcapillary culture(MC),classical culture(CC),rapid urease(CLO) test,and histopathologic examination(HE) were performed with biopsy samples.Homogenized biopsy samples were loaded into capillary tubes and incubated for 48 h at 37 ℃ without providing a microaerophilic environment.Additionally,three or four loops of the homogenized sample were inoculated in a ready-to-use selective medium(Becton Dickinson,Helicobacter Agar,Modified) specific for the isolation of H.pylori and incubated at 37 ℃ in a microaerophilic atmosphere provided by Campy Gen(Becton Dickinson,Gas Pack).Bacteria reproducing in microcapillary tubes were evaluated in an inverted microscope and also were evaluated after performing a CC with the content.Results obtained by CC,CLO test,and HE were compared with those of MC.The diagnostic performances of the methods used in this study were evaluated for specificity,sensitivity,positive predictive value(PPV),negative predictive value(NPV),and CI.RESULTS:H.pylori was found positive by CLO test +HE and/or CC culture in 26 patient antrum and corpus biopsy samples.In 25(25/26) patient biopsy samples,H.pylori was isolated by MCM,whereas in only 14(14/26) patient biopsy samples,H.pylori was isolatedby CC.CLO test and HE were found positive in 17(17/26) patient biopsy samples.Comparing the results of the isolation of H.pylori by MCM,CC,CLO test,and HE,the sensitivity of the MCM was found as 96%,the specificity as 80%,the PPV as 83%,the NPV as 95%,and the 95%CI as 0.76(χ2 =31.51,P < 0.01) whereas the sensitivity of the CC was found as 54%(χ2 =19.15,P < 0.01),and the sensitivity of the CLO test and HE were found as 65%(χ2 =25.26,P < 0.01).CONCLUSION:This new microcapillary cultivation method for H.pylori has high diagnostic sensitivity compared with CC,HE,and CLO tests. | Adil M Allahverdiyev Melahat Bagirova Reyhan Caliskan Hrisi Bahar Tokman Hayat Aliyeva Gokce Unal Olga Nehir Oztel Emrah Sefik Abamor Hilal Toptas Pelin Yuksel Fatma Kalayci Mustafa Aslan Yusuf Erzin Kadir Bal Bekir S Kocazeybek | 2015 | World Journal of Gastroenterology2015，21，9： | 2 |
| 2 | Comparison of quality of life and treatment efficacy in patients with chronic low back pain syndrome显示文摘 | Bagirova GG | 2006 | Ter Arkh2006，78，6： | 1 |
| 3 | The effect of Solco Trichovac on the vaginal microflora of patients with a papillomavirus infection assciated with a cervical intraepithelial neoplasm显示文摘 | KORSHUNOV V M KAFARSKAIA L I BAGIROVA M S | | 0，，： | 1 |
| 4 | Histochrome: a new original domestic drug显示文摘 | MISHCHENKO N P FEDOREEV S A BAGIROVA V L | 2003 | Pharm Chem J2003，37，1： | 1 |
| 5 | The effect of SolcoTfichovac on the vaginal microflora of patients with a papillomavirus infection associated with a cervical intraepithelial neoplasm 显示文摘 | KORSHUNOV V M KAFARSKAIA L I BAGIROVA M S H | 1994 | Zh Mikrobiol Epidemiol Immunobiol1994，，5： | 1 |
| 6 | Mutants of Phytophthora infestans resistant to dimethomorph fungicide显示文摘 | Bagirova S F Li A Z Dolgova A V | 2001 | J Russian Phytopathol Soc2001，2，1： | 1 |
| 7 | Utility of the microculture method in non-invasive samples obtained from an experimental murine model with asymptomatic leishmaniasis显示文摘 | Allahverdiyev AM Bagirova M Cakir-Koc R | 2012 | The American Journal of Tropical Medicine and Hygiene2012，87，1： | 1 |
| 8 | The effect of Sol-coTrichovae on the vaginal mieroflora of patients with a papillomavirus infection associated with a cervical intraepithelial neoplasm 显示文摘 | Korshunov VM Kafarskaia LI Bagirova MSH | 1994 | ZhMikrobi-ol Epidemiol Immunobiot1994，5，： | 1 |
| 9 | Enzymatic determination of phenols using peanut peroxidase 显示文摘 | BAGIROVA N A SHEKHOVTSOVA T N VAN HUYSTEE R B | 2001 | Talanta2001，55，6： | 1 |
| 10 | The effect of Solco Trichovac on the vaginal microEora of patients with a papillomavirus inl:etion associated with a cervical intraepithelial neo- plasm显示文摘 | KORSHUNOV V M KAFARSKAIA L 1 BAGIROVA M S H | 1994 | Zh Mikrobiol Epidemiol Inmmnobiol1994，，5： | 1 |
| 11 | The cf fect of Soleo Trichovac on the vaginal inicroflorn of patients with a papillomavirus infection associated with a cervical intraepitheli- al neo-plasm显示文摘 | Korshunov VM Kafarskaia LI Bagirova MSH | 1994 | Zh Mikrobiol Epidemiol Immunobiol1994，，5： | 1 |
| 12 | The effect of solco trichovac on the vaginal microflora of patients with a papillomavirus IFNection associated with a cervical intraepithelial neoplasm显示文摘 | Korshunov VM Kafarskaia LI Bagirova MSh | 1994 | Zh Mikrobiol Epidemiol Immunobiol1994，，5： | 1 |
| 13 | Histochrome:a New Original Domestic Drug显示文摘 | Fedoreev S A Bagirova V L | 2003 | Pharm Chem J2003，37，1： | 1 |
| 14 | Utility of the microculture method in non-invasive samples obtained from an experimental murine model with asymptomatic leishmaniasis显示文摘 | Allahverdiyev AM Bagirova M Cakir-Koe R et a1 | 2012 | Am J Trop Med Hyg2012，87，1： | 1 |
| 15 | The effect of Solcot richovac on the vaginal microflora of patients with a papillomavirus infection associated with a cervical intraepithelial neoplasm显示文摘 | Korshunov VM Kafarskaia LI Bagirova MS | 1994 | Zh Mikrobiol Epidemiol Immunobiol1994，5，： | 1 |
| 16 | The effect of Solco Trichovac on the vaginal microflora of patients with a papillomavirus infection assciated with a cervical intraepithelial neoplasm 显示文摘 | Korshunov VM Kafarskaia LI Bagirova MSH | 1995 | Zh Mikrobiol Epidemiol lmmunobiol1995，4，： | 1 |
| 17 | A new approach for development of vaccine against visceral leishmaniasis: Lipophosphoglycan and polyacrylic acid conjugates显示文摘Objective: To determine the antileishmanial vaccine effectiveness of lipophosphoglycan(LPG) and polyacrylic acids(PAA) conjugates on in vivo mice models.Methods: LPG molecule was isolated and purified from large-scale Leishmania donovani parasite culture. Protection efficacies of LPG alone, in combination with Freund's adjuvant, in a physical mixture and in conjugate(consisting of various LPG concentrations) with PAA, were comparatively determined by various techniques, such as cultivation with the micro-culture method, assessment of in vitro infection rates of peritoneal macrophages, determination of parasite load in liver with Leishman-Donovan Units, and detection of cytokine responses.Results: Obtained results demonstrated that the highest vaccine-mediated immune protection was provided by LPG-PAA conjugate due to all parameters investigated. According to the Leishman-Donovan Units results, the sharpest decline in parasite load was seen with a ratio of 81.17% when 35 mg LPG containing conjugate was applied. This value was 44.93% for the control group immunized only with LPG. Moreover, decreases in parasite load were 53.37%, 55.2% and 65.8% for the groups immunized with 10 mg LPG containing LPG-PAA conjugate, a physical mixture of the LPG–PAA, and a mixture of LPG + Freund's adjuvant, respectively. Furthermore, cytokine results supported that Th1 mediated protection occurred when mice were immunized with LPG-PAA conjugate.Conclusions: It has been demonstrated in this study that conjugate of LPG and PAA has an antileishmanial vaccine effect against visceral leishmaniasis. In this respect, the present study may lead to new vaccine approaches based on high immunogenic LPG molecule and adjuvant polymers in fighting against Leishmania infection. | Adil M.Allahverdiyev Rabia Cakir Koc Melahat Bagirova Serhat Elcicek Serap Yesilkir Baydar Olga Nehir Oztel Emrah Sefik Abamor Sezen Canim Ates Murat Topuzogullari Sevil Isoglu Dincer Zeynep Akdeste | 2017 | Asian Pacific Journal of Tropical Medicine2017，10，9： | 1 |
| 18 | The effect of Solco Trichovac on the vaginal microflora of patients with a papillomavirus IFNection associated with a cervical intraepithelial neoplasm显示文摘 | Korshunov VM Kafarskaia LI Bagirova MSh | 1994 | Zh Mikrobiol Epidemiol Immunobiol1994，5，： | 1 |
| 19 | The effect of Solco Trichovac on the vaginal microflora of patients with a papillomavirus infection associated with a cervical intra- epithelial neoplasm显示文摘 | Korshunov VM Kafarskaia LI Bagirova MSH | 1994 | Zh Mikrobiol Epidemiol Immunobiol1994，，5： | 1 |
| 20 | Antimicrobial effects of TiO2 and Ag2O nanoparticles against drug-resistant bacteria and leishmania parasites显示文摘 | Allahverdiyev Adil M Abamor Emrah Sefik Bagirova Malahat Rafailovich Miriam | 2011 | EN2011，，8： | 1 |
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