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| 1 | Translational pancreatic cancer research:a comparative study on patient-derived xenograft models显示文摘AIM To assess the viability of orthotopic and heterotopic patient-derived pancreatic cancer xenografts implanted into nude mice.METHODS This study presents a prospective experimental analytical follow-up of the development of tumours in mice upon implantation of human pancreatic adenocarcinoma samples. Specimens were obtained surgically from patients with a pathological diagnosis of pancreatic adenocarcinoma. Tumour samples from pancreatic cancer patients were transplanted into nude mice in three different locations(intraperitoneal, subcutaneous and pancreatic). Histological analysis(haematoxylin-eosin and Masson's trichrome staining) and immunohistochemical assessment of apoptosis(TUNEL), proliferation(Ki-67), angiogenesis(CD31) and fibrogenesis(α-SMA) were performed. When a tumour xenograft reached the target size, it was reimplanted in a new nude mouse. Three sequential tumour xenograft generations were generated(F1, F2 and F3).RESULTS The overall tumour engraftment rate was 61.1%. The subcutaneous model was most effective in terms of tissue growth(69.9%), followed by intraperitoneal(57.6%) and pancreatic(55%) models. Tumour development was faster in the subcutaneous model(17.7 ± 2.6 wk) compared with the pancreatic(23.1 ± 2.3 wk) and intraperitoneal(25.0 ± 2.7 wk) models(P = 0.064). There was a progressive increase in the tumour engraftment rate over successive generations for all three models(F1 28.1% vs F2 71.4% vs F3 80.9%, P < 0.001). There were no significant differences in tumour xenograft differentiation and cell proliferation between human samples and the three experimental models among the sequential generations of tumour xenografts. However, a progressive decrease in fibrosis, fibrogenesis, tumour vascularisation and apoptosis was observed in the three experimental models compared with the human samples. All three pancreatic patient-derived xenograft models presented similar histological and immunohistochemical characteristics.CONCLUSION In our experience, the faster development andgreatest number of viable xenografts could make the subcutaneous model the best option for experimentation in pancreatic cancer. | Mercedes Rubio-Manzanares Dorado Luis Miguel Marín Gómez Daniel Aparicio Sánchez Sheila Pereira Arenas Juan Manuel Praena-Fernández Juan Jose Borrero Martín Francisco Farfán López Miguel ángel Gómez Bravo Jordi Muntané Relat Javier Padillo Ruiz | 2018 | World Journal of Gastroenterology2018，24，7： | 2 |
| 2 | 14-day triple, 5-day concomitant, and 10-day sequential therapies for Helicobacter pylori infection in seven Latin American sites: a randomised trial显示文摘 | E Robert Greenberg Garnet L Anderson Douglas R Morgan Javier Torres William D Chey Luis Eduardo Bravo Ricardo L Dominguez Catterina Ferreccio Rolando Herrero Eduardo C Lazcano-Ponce María Mercedes Meza-Montenegro Rodolfo Pe?a Edgar M Pe?a Eduardo Salazar- | 2011 | The Lancet2011，，9790： | 2 |
| 3 | Extramedullary plasmocytoma associated with a massive deposit of amyloid in the duodenum显示文摘We report a rare case of extramedullary plasmocytoma associated with a massive deposit of amyloid in the duodenum.A 72-year-old Japanese man was admitted to our hospital presenting with a 3-mo history of epigastric pain, vomiting and weight loss.On computed tomography(CT) a wall thickening of the fourth part of the duodenum was observed.Multiple biopsies obtained from the lesion showed inflltration of plasma cells and lymphocytes, but they were not conclusive.The patient underwent resection of the lesion and, on histopathological examination, the lesion consisted of a dense and diffuse inflltrate of plasma cells and a few admixed lymphocytes with reactive follicles extending to the muscular propria.An extensive deposition of amyloid was also observed.Immunohistochemical stains revealed that a few plasmacytoid cells showed λ light chain staining, though most were κ light chain positive.These cells also were positive for CD138 and CD56 but negative for CD20 and CD79.The flndings were consistent with extramedullary plasmocytomaassociated with a massive deposit of amyloid in duodenum.A subsequent workup for multiple myeloma was completely negative.The patient showed no signs of local recurrence or dissemination of the disease after 12 mo follow-up.Because of the association of plasmocytoma and amyloidosis, the patient must be followed up because of the possible systemic involvement of the neoplasm and amyloidosis in future. | Fabiana Pirani Carneiro Maria de Nazareth Machado Sobreira Lívia Bravo Maia Alesso Cervantes Sartorelli Luiz Eduardo de Almeida Prado Franceschi Mauro Brito Brando Bárbara Wosnjuk Calaca Fernando Silva Lustosa Joo Vieira Lopes | 2009 | World Journal of Gastroenterology2009，15，28： | 2 |
| 4 | E6 molecular variants of human papillomavirus (HPV) type 16: An updated and unified criterion for clustering and nomenclature显示文摘 | Antonio Huertas-Salgado Diana C. Martín-Gámez Pablo Moreno Raúl Murillo María Mercedes Bravo Luisa Villa Mónica Molano | 2010 | Virology2010，，： | 2 |
| 5 | Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance显示文摘 | Bravo L | 1998 | Nutrition Reviews1998，56，： | 1 |
| 6 | Laparoscopic adrena lectomy: th e preferred choice? A comparison to open adrenalectomy显示文摘 | HAMILTON B D BRAVO E L | 1998 | J Urol1998，160，： | 1 |
| 7 | Polyphenols:chemistry,dietary source,metabolism,and nutritional significance显示文摘 | Bravo L | 1998 | Nutr Rev1998，56，11： | 1 |
| 8 | Characterization of antifreeze activity in antarctic plants显示文摘 | BRAVO L A GRIFFITH M | 2005 | Journal of Experimental Botany2005，56，414： | 1 |
| 9 | Na/Pb, Na/Cd and Pb/Cd exchange on a low iron Texas bentonite in the presence of competing H + ion 显示文摘 | Breen C Bravo M B Madrid L | 1999 | Colloids and Surfaces A: Physicochemical and Engineering Aspects1999，155，23： | 1 |
| 10 | Surgical site infection: rates, etiology and resistance patterns to antimicrobials among strains isolated at Rio de Janeiro University Hospital显示文摘 | Santos K R Fonseca L S Bravo Neto G P | 1997 | Infection1997，25，4： | 1 |
| 11 | Polyphenols: chemistry, dietary sources,metabolism, and nutritional significance 显示文摘 | Bravo L | 1998 | Nutr Rev1998，56，： | 1 |
| 12 | Detecting Bruises on ‘Golden Delicious’ Apples using Hyperspectral Imaging with Multiple Wavebands显示文摘 | Juan Xing Cédric Bravo Pál T. Jancsók Herman Ramon Josse De Baerdemaeker | 2004 | Biosystems Engineering2004，，1： | 1 |
| 13 | Polyphenols: chemistry, dietary sources, metabolism and nutritional significance 显示文摘 | Bravo L | 1998 | Nutr Rev1998，56，11： | 1 |
| 14 | Validation of an immunologic diagnostic kit for infectious by Trichomonas vaginalis-Candida spp and Gardnerella vaginalis显示文摘 | Bravo A B Miranda L S Lima O F | 2009 | Diaqn Mierobiol Infect Dis2009，63，3： | 1 |
| 15 | Mass transfer in gauze packings显示文摘 | Bravo J L Rocha J A Fair J R | 1985 | Hydrocarbon Processing1985，64，： | 1 |
| 16 | Treatment of dental alveolitis with 5% propolis tincture 显示文摘 | BRAVO VENERO A V DI'AZ GARCI'A L M GONZALEZ L A | 2012 | Revista Cubana de Farmacia2012，46，1： | 1 |
| 17 | Pressure drop instructure packings显示文摘 | Bravo J L Rocha J A Fair J R | 1986 | Hydrocarbon Processing1986，3，： | 1 |
| 18 | Stability limits of milling considering the flexibility of the workpiece and the machine显示文摘 | Bravo U Altuzarra O Lopez deLacalle L N | 2005 | International Journal of Machine Tools and Manufacture2005，45，15： | 1 |
| 19 | Polyphenols: chemistry, dietary sources, metabo- lism and nutritional significance显示文摘 | Bravo L | 1998 | Nutrit Rev1998，56，11： | 1 |
| 20 | Estimating the reliability of continuous measures with cronbach's alpha or the intraclass correlation coefficient:toward the integration of two traditions显示文摘 | Bravo G Potvin L | 1991 | J Clin Epidemiol1991，44，45： | 1 |
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