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| 1 | A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs显示文摘African swine fever(ASF)is a devastating infectious disease in swine that is severely threatening the global pig industry.An efficacious vaccine is urgently required.Here,we used the Chinese ASFV HLJ/18 as a backbone and generated a series of genedeleted viruses.The virulence,immunogenicity,safety,and protective efficacy evaluation in specific-pathogen-free pigs,commercial pigs,and pregnant sows indicated that one virus,namely HLJ/18-7GD,which has seven genes deleted,is fully attenuated in pigs,cannot convert to the virulent strain,and provides complete protection of pigs against lethal ASFV challenge.Our study shows that HLJ/-18-7GD is a safe and effective vaccine against ASFV,and as such is expected to play an important role in controlling the spread of ASFV. | Weiye Chen Dongming Zhao Xijun He Renqiang Liu Zilong Wang Xianfeng Zhang Fang Li Dan Shan Hefeng Chen Jiwen Zhang Lulu Wang Zhiyuan Wen Xijun Wang Yuntao Guan Jinxiong Liu Zhigao Bu | 2020 | Science China(Life Sciences)2020，63，5： | 72 |
| 2 | Syntheses, structures and magnetic properties of three Co(Ⅱ)coordination architectures based on a flexible multidentate carboxylate ligand and different N-donor ligands显示文摘Three new cobalt complexes,{[Co5(tci)2(bimb)3(3-O)2(H2O)2]·3DMF·4H2O}n(1),{[Co3(tci)2(bib)]·2DMF·2H2O}n(2)and{[Co(Htci)(bpea)0.5]·H2O}n(3)(H3tci=tris(2-carboxyethyl)isocyanurate,bimb=4,4′-bis(imidazol-1-yl)biphenyl,bib=1,4-bis(imidazol-1-yl)benzene,bpea=1,2-bis(4-pyridyl)ethane,DMF=N,N′-dimethylformamide),have been successfully synthesized through the assembly of Co(II)ions,H3tci and different N-donor ligands,respectively.All complexes were structurally characterized by single crystal X-ray diffraction,elemental analyses,IR spectra,thermogravimetric(TG)analyses and X-ray powder diffraction(XRPD).Complex 1 exhibits a 3D three-fold parallel interpenetrated 3D→3D structure with(65·8)CdSO4topology.Complex 2 is built from[Co3(2-Ocarboxyl)2(CO2)4]clusters and linear bib ligands,displaying a two-fold parallel interpenetrated(3,8)-connected(43)2(46·618·84)topology,while complex 3 is a 3D pillar-layered structure involving an infinite–Co–(2-Ocarboxyl)(CO2)–Co–chain.The diverse structures of the three complexes indicate that the skeletons of different N-donor ligands play an important role in the assembly of such different frameworks.In addition,magnetic investigation indicates that besides spin-orbit coupling of Co(II)ions,there exist antiferromagnetic exchange interactions in Co5and Co3clusters of 1 and 2,respectively. | TIAN Dan LIU SuiJun ZHANG DaShuai CHANG Ze HU TongLiang BU XianHe | 2013 | Science China Chemistry2013，56，12： | 5 |
| 3 | Noncontrast-enhanced MRI-based Noninvasive Score for Portal Hypertension(CHESS1802):An International Multicenter Study显示文摘Background and Aims:This study aimed to determine the performance of the non-invasive score using noncontrastenhanced MRI(CHESS-DIS score)for detecting portal hy-pertension in cirrhosis.Methods:In this international multicenter,diagnostic study(ClinicalTrials.gov,NCT03766880),patients with cirrhosis who had hepatic venous pressure gradient(HVPG)measurement and noncontrast-enhanced MRI were prospectively recruited from four university hospitals in China(n=4)and Turkey(n=1)between December 2018 and April 2019.A cohort of patients was retrospectively recruited from a university hospital in Italy between March 2015 and November 2017.After segmentation of the liver on fat-suppressed T1-weighted MRI maps,CHESS-DIS score was calculated automatically by an in-house developed code based on the quantification of liver surface nodularity.Results:A total of 149 patients were included,of which 124 were from four Chinese hospitals(training cohort)and 25 were from two international hospitals(validation cohort).A positive correlation between CHESS-DIS score and HVPG was found with the correlation coefficients of 0.36(p<0.0001)and 0.55(p<0.01)for the training and validation cohorts,respectively.The area under the receiver operating characteristic curve of CHESS-DIS score in detection of clinically significant portal hypertension(CSPH)was 0.81 and 0.9 in the training and validation cohorts,respectively.The intra-class correlation coefficients for assessing the inter-and intra-observer agreement were 0.846 and 0.841,respectively.Conclusions:A non-invasive score using noncontrast-enhanced MRI was developed and proved to be significantly correlated with invasive HVPG.Besides,this score could be used to detect CSPH in patients with cirrhosis. | Yanna Liu Tianyu Tang NecatiÖrmeci Yifei Huang Jitao Wang Xiaoguo Li Zhiwei Li Weimin An Dengxiang Liu Chunqing Zhang Changchun Liu Jinqiang Liu Chuan Liu Guangchuan Wang Cristina Mosconi Alberta Cappelli Antonio Bruno Seray Akçalar EmrecanÇelebioğlu EvrenÜstüner Sadık Bilgiç Zeynep Ellik ÖzgünÖmer Asiller Lei Li Haijun Zhang Ning Kang Dan Xu Ruiling He Yan Wang Yang Bu Ye Gu Shenghong Ju Rita Golfieri Xiaolong Qi | 2021 | Journal of Clinical and Translational Hepatology2021，9，6： | 3 |
| 4 | Development and Application of a PCR Approach for Detection of Bovis, Sheep, Pig, and Chicken Derived Materials in Feedstuff显示文摘A PCR method for detection of bovis, sheep, pig, and chicken derived materials in feedstuff was established, and the existing method was improved according to the research on general primer and species-specific primers. First, general primer designed according to 16S rRNA gene sequence of bovis, sheep, pig, chicken, fish, and horse mtDNA was used for primary detection of animal derived materials in feedstuff. Species-specific primers designed according to conserved sequence of mtDNA of bovis, sheep, pig, and chicken were used for amplification of a 271, 274, 149, and 266 bp fragment, respectively. Further confirmation of the detection result was then carried out. PCR method for detection of animal derived materials in unknown feedstuff was developed by using general primer, relevant PCR system, and PCR condition. Also a PCR method for detection of each species (bovines, sheep, pig, and chicken) was designed by using our species- specific primers. High sensitivity and specificity of our method were confirmed with a minimum detection level of 0.1%. Method for detection of animal derived materials in this research is not only cheap and easy for operation but also precise and reliable results can be obtained. It could be one of the effective methods for the detection of animal derived materials in feedstuff. | LUO Jia-qin WANG Jia-qi BU Deng-pan LI Dan WANG Li WEI Hong-yang ZHOU Ling-yun | 2008 | Agricultural Sciences in China2008，7，10： | 2 |
| 5 | Biotin–streptavidin enzyme-linked immunosorbent assay for detecting Tetrabromobisphenol A in electronic waste显示文摘 | Dan Bu Huisheng Zhuang Xinchu Zhou Guangxin Yang | 2014 | Talanta2014，，： | 1 |
| 6 | Small Angle X-ray Scattering Study of PS Conformation in Tetrahydrofuran Solvent with Gas Anti-solvent显示文摘Small angle X-ray scattering (SAXS) was used to study the effect of dissolved CO2 on the conformation of polystyrene (PS) in PS/tetrahydrofuran(THF) solution at 308.15 K and at pressures up to 3 MPa. The cloud pressure and the expansion curve of the solution were also determined. The dependence of the conformation on pressure was discussed. | Dan LI Zhi Min LIU Guan Ying YANG Qing HUO Bu Xing HAN Yi LIU Zhong Hua WU Bao Zhong DONG | 2000 | Chinese Chemical Letters2000，11，12： | 1 |
| 7 | Characterization and activity of visible-light-driven Ti O2photocatalyst codoped with lanthanum and iodine显示文摘 | LI Ling ZHUANG Hui-sheng BU Dan | 2011 | Applied Surface Science2011，257，4： | 1 |
| 8 | Preparation of PMMA Foam by Supercritical CO_2 with Ethanol显示文摘Supercritical CO2 with ethanol as blowing agent foamed poly(methylmethacrylate) (PMMA) fiber at 308.15K and in the pressure range from 12-15MPa. The foam structure was detected using scanning electron microscope. It was found that the concentration of ethanol in the fluid is a major parameter to affect the foam structure. | Zhi Min LIU, GuanYing YANG, Dan LI, Bu Xing HAN* Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080 | 2001 | Chinese Chemical Letters2001，12，2： | 0 |
| 9 | Polymerization of Styrene in Supercritical CO_2-swollenPoly(ethylene terephthlate)显示文摘Polystyrene/Poly(ethylene terephthlate) (PET) blends have been prepared by theheterogeneous free-radical polymerization of styrene within supercritical carbon dioxide-swollenPET substrates. Composition of the blends and the average molecular weight of polystyrene inthe blends can be controlled by equilibration time and reaction condition. | Dan LI Bu Xing HAN Hai Ke YAN(Institute of chemistry. The Chinese Academy of Sciences, Beijing 100080) | 1999 | Chinese Chemical Letters1999，10，10： | 0 |
| 10 | Photocatalytic Degradation of Acid Blue 62 over La/I/TiO2 Nanocomposite Photocatalyst under Simulated Sunlight显示文摘 | LI Ling ZHUANG Hui-sheng BU Dan | 2010 | Journal of Donghua University(English Edition)2010，27，6： | 0 |
| 11 | Immunogenicity of a recombinant VSV-Vectored SARS-CoV vaccine induced robust immunity in rhesus monkeys after single-dose immunization显示文摘Severe acute respiratory syndrome(SARS)is a highly contagious zoonotic disease caused by SARS coronavirus(SARS-Co V).Since its outbreak in Guangdong Province of China in 2002,SARS has caused 8096 infections and774 deaths by December 31st,2003.Although there have been no more SARS cases reported in human populations since 2004,the recent emergence of a novel coronavirus disease(COVID-19)indicates the potential of the recurrence of SARS and other coronavirus disease among humans.Thus,developing a rapid response SARS vaccine to provide protection for human populations is still needed.Spike(S)protein of SARS-Co V can induce neutralizing antibodies,which is a pivotal immunogenic antigen for vaccine development.Here we constructed a recombinant chimeric vesicular stomatitis virus(VSV)VSVΔG-SARS,in which the glycoprotein(G)gene is replaced with the SARS-Co V S gene.VSVΔG-SARS maintains the bullet-like shape of the native VSV,with the heterogeneous S protein incorporated into its surface instead of G protein.The results of safety trials revealed that VSVΔG-SARS is safe and effective in mice at a dose of 1×10^(6)TCID_(50).More importantly,only a single-dose immunization of 2×10^(7)TCID_(50)can provide high-level neutralizing antibodies and robust T cell responses to non-human primate animal models.Thus,our data indicate that VSVΔG-SARS can be used as a rapid response vaccine candidate.Our study on the recombinant VSV-vectored SARS-Co V vaccines can accumulate experience and provide a foundation for the new coronavirus disease in the future. | Dan Shan Xiaoyan Tang Renqiang Liu Dan Pan Xijun Wang Jinying Ge Zhiyuan Wen Zhigao Bu | 2022 | Virologica Sinica2022，37，2： | 0 |
| 12 | Electrochemical Oxidation Dearomatization of Anisol Derivatives toward Spiropyrrolidines and Spirolactones显示文摘Spiro compounds are widely prevalent in biological activities and natural products.However,developing new strategies for their efficient synthesis and derivatization remains a challenge.Outstanding progress has been made in the synthesis of spiro compounds through dearomatization of aromatic compounds,most of them are mediated by the hypervalent iodine reagents.Herein,we report a method of anodic oxidation spiroamination and spirolactonization of anisole derivatives with concomitant cathodic reduction of protons in the absence of hypervalent iodine reagents.A wide variety of spiropyrrolidines and spirolactones with diverse functional groups made useful scaffolds in this transformation,with yields up to 97%.Moreover,hectogram-scale synthesis could supply target product with 83% yield in a flow electrochemical cell using carbon paper as the anode and nickel plate as the cathode,demonstrating the potential application of this method. | Cui Zhang Faxiang Bu Chulin Zeng Dan Wang Lijun Lu Heng Zhang Aiwen Lei | 2022 | CCS Chemistry2022，4，4： | 0 |
| 13 | Research and Implementation of the SDN Resources Transaction Process Based on Trust Mechanism显示文摘SDN network as new network architecture,can address a range of issues of the current internet effectively,this paper in the view of SDN network resources' allocation,for decreasing resources waste and achieving a reasonable distribution of SDN resources,come up with a suitable resource price negotiation algorithm based on trust mechanism in SDN network environment.According to the characteristics of the trust mechanism,simulating on the unit utility and equivalent price of SDN network resources in three aspects(remove individual malicious nodes,remove most malicious nodes and remove all malicious nodes),through the above simulation,combined trust mechanism with MCDAM algorithm,can form a weighing system which pricing and trust comprehensive competitive,so that the whole SDN trading environment become safer and more stable.So trust mechanism as the patch of SDN pricing negotiation algorithm.Finally,propose the planned trading model based on trust mechanism as a management method of SDN trading. | ZHUGE Bin PENG Dan BU Xiaobo WANG Weiming | 2016 | China Communications2016，13，S1： | 0 |
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