|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 2 | Technical outcomes of sentinel-lymph-node resection and conventional axillary-lymph-node dissection in patients with clinically node-negative breast cancer: results from the NSABP B-32 randomised phase III trial显示文摘 | David N Krag Stewart J Anderson Thomas B Julian Ann M Brown Seth P Harlow Takamaru Ashikaga Donald L Weaver Barbara J Miller Lynne M Jalovec Thomas G Frazier R Dirk Noyes André Robidoux Hugh MC Scarth Denise M Mammolito David R McCready Eleftherios P Mamo | 2007 | Lancet Oncology2007，，10： | 2 |
| 3 | Intestinal inflammation and activation of sensory nerve pathways:a functional and morphological study in the nematode infected rat 显示文摘 | Giorgio R Barbara G Blennerhassett P | 2001 | Gut2001，49，： | 2 |
| 4 | Glucagon-like peptide 2:A nutrientresponsive gut growth factor显示文摘 | Douglas G B Yvette P Barbara S | 2001 | The Journal of Nutrition Bethesda2001，131，3： | 1 |
| 5 | Uranium reduction by desulfovibrio desulfuricans strain G20 and a cytochrome c3 mutant 显示文摘 | Rayford B P Darren M G Barbara J R | 2002 | Applied and Environmental Microbiology2002，68，6： | 1 |
| 6 | Elderly patients on dialysis: Epidemiology of an epidemic显示文摘 | GIUSEPPE P MARIO S BARBARA P G | 1996 | Nephrol Dail Transplant1996，11，9： | 1 |
| 7 | Multidrug Resistance Proteins\sMRP3,MRP1,and MRP2 in Lung Cancer:Correlation of Protein Levels\swith Drug Response and Messenger RNA Levels显示文摘 | Leah C Barbara G Susan P | 2001 | Clinical Cance\sResearch2001，7，： | 1 |
| 8 | Intestinal inflammation and activation of sensory nerve pathways: a functional and morphological study in the nematode infected rat显示文摘 | Giorgio Rde Barbara G Blennerhassett P | 2001 | Gut2001，49，： | 1 |
| 9 | A fuzzy echelon approach for inventrory management in supply chains显示文摘 | ILARIA G PIERAOLO P BARBARA S | 2003 | European Journal of Operational Research2003，149，1： | 1 |
| 10 | Adenomyosis and re- productive performance after surgery for rectovaginal and colorectal endometriosis : a systematic review and recta- analy- sis显示文摘 | Vercellini P Consonni D Barbara G | 2014 | Reprod Biomed Online2014，28，6： | 1 |
| 11 | Impaired intestinal barrier integrity in the colon of patients with irritable bowel syndrome:involvement of soluble mediators显示文摘 | Piche T Barbara G Aubert P | 2009 | Gut2009，58，2： | 1 |
| 12 | The psychosocial impact of macular degeneration显示文摘 | Williams Rebecca A Brody Barbara M P H Thomas Ronald G | 1998 | Archives of Ophthalmology1998，116，4： | 1 |
| 13 | Interproeedural Def-Use associations for C Systems with Single LevelPointers显示文摘 | Hemant D P William P L Barbara G R | | IEEE Transactions on Software Engineering0，20，5： | 1 |
| 14 | Detection of substance P immunoreaetivity in human peripheral leukoeytes显示文摘 | De Giorgio R Tazzari P L Barbara G | 1998 | J neuroimmunol1998，82，： | 1 |
| 15 | Multidrug Resistance Proteins MRP3,MRP1,and MRP2 in Lung Cancer:Correlation of Protein Levels with Drug Response and Messenger RNA Levels显示文摘 | Leah C Barbara G Susan P | 2001 | Clinical Cancer Research2001，7，： | 1 |
| 16 | A fuzzy echelon approach for inventory management in supply chains显示文摘 | ILATIA G PIERPAOLO P BARBARA S | 2003 | European Journal of Operational Research2003，149，： | 1 |
| 17 | Pluripotency regulators in human mesenchymal stem cells:expression of NANOG but not of OCT-4 and SOX-2 显示文摘 | Enrico P Barbara G Ivana M | 2011 | Stem? Cells Dev2011，20，5： | 1 |
| 18 | Proton-transfer kinetics of3-hydroxyflavone:Solvent effects显示文摘 | Strandjord A J G Barbara P F | 1985 | J Phys Chem1985，56，8： | 1 |
| 19 | Impaired intestinal barrier integrity in the colon of patients with irritable bowel syndrome: involvement of soluble mediators 显示文摘 | PICHE T BARBARA G AUBERT P | 2009 | Gut2009，58，2： | 1 |
| 20 | The psychosocial impact of macular degeneration显示文摘 | Williams Rebecca A Brody Barbara M P H Thomas Ronald G | 1998 | Archives of Ophthalmology1998，116，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员