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| 1 | Lymphocyte subsets in alcoholic liver disease显示文摘AIM:To compare lymphocyte subsets between healthy controls and alcoholics with liver disease.METHODS:The patient cohort for this study included individuals who were suspected to have alcoholic liver disease(ALD) and who had undergone liver biopsy(for disease grading and staging,doubts about diagnosis,or concurrent liver disease;n = 56).Normal controls included patients who were admitted for elective cholecystectomy due to non-complicated gallstones(n = 27).Formalin-fixed,paraffin-embedded liver biopsy specimens were sectioned and stained with hematoxylin and eosin and Perls' Prussian blue.The non-alcoholic steatohepatitis score was used to assess markers of ALD.Lymphocyte population subsets were determined by flow cytometry.T lymphocytes were identified(CD3+),and then further subdivided into CD4+ or CD8+ populations.B lymphocytes(CD19+) and natural killer(NK) cell numbers were also measured.In addition to assessing lymphocyte subpopulation differences between ALD patients and controls,we also compared subsets of alcoholic patients without cirrhosis or abstinent cirrhotic patients to normal controls.RESULTS:The patient cohort primarily consisted of older men.Active alcoholism was present in 66.1%.Reported average daily alcohol intake was 164.9 g and the average lifetime cumulative intake was 2211.6 kg.Cirrhosis was present in 39.3% of the patients and 66.1% had significant fibrosis(perisinusoidal and portal/periportal fibrosis,bridging fibrosis,or cirrhosis) in their liver samples.The average Mayo end-stage liver disease score was 7.6.No hereditary hemochromatosis genotypes were found.ALD patients(n = 56) presented with significant lymphopenia(1.5 × 109/L ± 0.5 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P < 0.0001),due to a decrease in all lymphocyte subpopulations,except for NK lymphocytes:CD3+(1013.0 ± 406.2/mm3 vs 1523.0 ± 364.6/mm3,P < 0.0001),CD4+(713.5 ± 284.7/mm3 vs 992.4 ± 274.7/mm3,P < 0.0001),CD8+(262.3 ± 140.4/mm3 vs 478.9 ± 164.6/mm3,P < 0.0001),and CD19+(120.6 ± 76.1/mm3 vs 264.6 ± 88.0/mm3,P < 0.0001).CD8+ lymphocytes suffered the greatest reduction,as evidenced by an increase in the CD4+/CD8+ ratio(3.1 ± 1.3 vs 2.3 ± 0.9,P = 0.013).This ratio was associated with the stage of fibrosis on liver biopsy(rs = 0.342,P = 0.01) and with Child-Pugh score(rs = 0.482,P = 0.02).The number of CD8+ lymphocytes also had a positive association with serum ferritin levels(rs = 0.345,P = 0.009).Considering only patients with active alcoholism but not cirrhosis(n = 27),we found similar reductions in total lymphocyte counts(1.8 × 109/L ± 0.3 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P = 0.018),and in populations of CD3+(1164.7 ± 376.6/mm3 vs 1523.0 ± 364.6/mm3,P = 0.001),CD4+(759.8 ± 265.0/mm3 vs 992.4 ± 274.7/mm3,P = 0.003),CD8+(330.9 ± 156.3/mm3 vs 478.9 ± 164.6/mm3,P = 0.002),and CD19+(108.8 ± 64.2/mm3 vs 264.6 ± 88.0/mm3,P < 0.0001).In these patients,the CD4+/CD8+ ratio and the number of NK lymphocytes was not significantly different,compared to controls.Comparing patients with liver cirrhosis but without active alcohol consumption(n = 11),we also found significant lymphopenia(1.3 × 109/L ± 0.6 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P < 0.0001) and decreases in populations of CD3+(945.5 ± 547.4/mm3 vs 1523.0 ± 364.6/mm3,P = 0.003),CD4+(745.2 ± 389.0/mm3 vs 992.4 ± 274.7/mm3,P = 0.032),CD8+(233.9 ± 120.0/mm3 vs 478.9 ± 164.6/mm3,P < 0.0001),and CD19+(150.8 ± 76.1/mm3 vs 264.6 ± 88.0/mm3,P = 0.001).The NK lymphocyte count was not significantly different,but,in this group,there was a significant increase in the CD4+/CD8+ ratio(3.5 ± 1.3 vs 2.3 ± 0.9,P = 0.01).CONCLUSION:All patient subsets presented with decreased lymphocyte counts,but only patients with advanced fibrosis presented with a significant increase in the CD4+/CD8+ ratio. | Luís Costa Matos Paulo Batista Nuno Monteiro Joo Ribeiro Maria A Cipriano Pedro Henriques Fernando Giro Armando Carvalho | 2013 | World Journal of Hepatology2013，5，2： | 4 |
| 2 | Flavonoids and mannan oligosaccharides in broiler diets显示文摘 | Batista L S | 2007 | Brazilian Journal of Poultry Science2007，9，1： | 1 |
| 3 | Microcystin-LR Causes the Collapse of Actin Filaments in Primary Human Hepatocytes显示文摘 | Batista T Sousa G D Suput S | 2003 | Aquatic Toxicology2003，65，： | 1 |
| 4 | Prediction of liquidliquid equilibrium for systems of vegetable oils, fatty acids, and ethanol显示文摘 | Batista E Monnerat S Stragevitch L | 1999 | J Chem Eng Data1999，44，6： | 1 |
| 5 | High efficiency steam reforming of ethanol by cobalt-based catalysts显示文摘 | Batista M S Santos R K S M Assaf E M | 2004 | J Power Sources2004，134，1： | 1 |
| 6 | Apoptosis inmalignant glioma cells triggered by the temozolomide-inducedDNA lesion O6-methylguanine显示文摘 | Roos W P Batista LFZ Naumann S | 2007 | Oncogene2007，26，： | 1 |
| 7 | Characterization of the activity and stability of supported cobalt catalysts for the steam reforming of ethanol显示文摘 | Batista M S Santos R K S M Assaf E M | 2003 | J Power Sources2003，24，1： | 1 |
| 8 | Photochemical degradation of sulfadiazine,sulfamerazine and sulfamethazine:Relevance of concentration and heterocyclic aromatic groups to degradation kinetics显示文摘 | Ana Paula S Batista Flavio Chiquetto C Pires Antonio Carlos S C Teixeira | 2014 | Journal of Photochemistry and Photobiology A:Chemistry2014，28，： | 1 |
| 9 | Densification behavior, microstructure evolution, and wear performance ofselective laser melting processed commercially pure titanium 显示文摘 | GU D D HAGEDOMRN Y C MEINERS W MENG G B BATISTA R J S WISSENBACH K POPRAWE R | 2012 | Acta Materialia2012，60，9： | 1 |
| 10 | Isolation and characterization of biosurfactant/bioemulsifier-producing bacteria from petroleum contaminated sites显示文摘 | Batista S B Mounteer A H Amorim F R | 2006 | Bioresource Technology2006，97，： | 1 |
| 11 | Eucalyptus hydrolysate detoxification with activated charcoal adsorption or ion-exchange resins for xylitol production显示文摘 | LARISSA C BATISTA A S SOLENZAL A I N | 2004 | Process Biochemistry2004，39，： | 1 |
| 12 | Endothelial FAK is required for tumour angiogenesis 显示文摘 | Tavora B Batista S Reynolds LE | 2010 | EMBO Molecular Medicine2010，2，12： | 1 |
| 13 | The Pht1;9 and Pht1;8 transporters mediate inorganic phosphate acquisition by the Arabidopsis thaliana root during phosphorus starvation显示文摘 | E. Remy T. R. Cabrito R. A. Batista M. C. Teixeira I. Sá‐Correia P. Duque | 2012 | New Phytologist2012，，2： | 1 |
| 14 | High HIF-1α expression genotypes in oral lichen planus显示文摘 | Carlos Alberto Carvalho Fraga Lucas Rodrigues Alves Luciano Marques-Silva Adriana Alkmim Sousa Antonio Sérgio Barcala Jorge Sabrina Ferreira Jesus Daniel Nogueira Vilela Ugo Borges Pinheiro Kimberly Marie Jones Alfredo Maurício Batista Paula André Luiz Se | 2013 | Clinical Oral Investigations2013，，9： | 1 |
| 15 | structural characteriza- tion and properties of water-soluble N-( γ-propanoyl-amino acid)-chitosans 显示文摘 | COMES P GOMES C A R BATISTA M K S | 2008 | Carbohydrate Polymers2008，71，1： | 1 |
| 16 | Synthesis of new fluorescent 2-(2',2”-bithienyl)-1,3-benzothiazoles显示文摘 | Batista R M F Costa S P G Raposo M M M | 2004 | Tetrahedron Lett2004，45，13： | 1 |
| 17 | Higher number of Helicobacter pylori CagA EPIYA C phosphorylation sites increases the risk of gastric cancer, but not duodenal ulcer显示文摘 | BATISTA S A ROCHA G A ROCHA A M | 2011 | BMC Microbiol2011，11，： | 1 |
| 18 | Synthe-sis,structural characterization and properties of water-solubleN-(γ-propanoyl-amino acid)-chitosans显示文摘 | GOMES P GOMES C A R BATISTA M K S | 2008 | Carbohydrate Poly-mers2008，71，1： | 1 |
| 19 | Pesticides in Portuguese surface and ground waters显示文摘 | Cerejeira M J Viana P Batista S | 2003 | Water Research2003，37，5： | 1 |
| 20 | Experimental colonization of piglets andgilts with systemic strains of HaemoPhilus Parasuis and StrePtococcus suis to prevent disease显示文摘 | Oliveira S Batista L Torremorell | 2011 | Can J vet Res2011，65，3： | 1 |
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