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| 1 | BRIF and CARIF progress显示文摘China Institute of Atomic Energy (CIAE) is currently constructing Beijing rare ion beam facility (BRIF) and is proposing China advanced rare ion beam facility (CARIF). This paper is aiming at introducing the progress of BRIF project and the con ceptual design CARIF. The ISOL type facility BRIF under construction is composed of a 100 MeV 300 ?A proton cyclotron, an ISOL with mass resolution of 20000, and a super-conducting LINAC of 2 MeV/q, and will be commissioned in 2013. CARIF facility proposed is planned to use both ISOL and PF techniques. It is based on a China advanced research reactor CARR that was critical, with ISOL separation of fission fragment, post acceleration to 150 MeV/u, and fragmentation of neu tron-rich fission fragment beam like 132Sn. Such unique combination will allow CARIF to deliver beam intensity better than the best world facilities by more than one order of magnitude. | LIU WeiPing, LI ZhiHong, BAI XiXiang, WANG YouBao, GUO Bing, PENG ChaoHua, YANG Yi, SU Jun, CUI BaoQun, ZHOU ShuHua, ZHU ShengYun, XIA HaiHong, GUAN XiaLing, ZENG Sheng, ZHANG HuanQiao, CHEN YongShou, TANG HongQing, HUANG Li & FENG BeiYuan China Institute of Atomic Energy, P. O. Box 275(1), Beijing 102413, China | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，S1： | 8 |
| 2 | Insulin-like growth factor 1-induced enolase 2 deacetylation by HDAC3 promotes metastasis of pancreatic cancer显示文摘Enolase 2(ENO2)is a key glycolytic enzyme in the metabolic process of glycolysis,but its potential function in pancreatic ductal adenocarcinoma(PDAC)is unclear.In this study,we observed a significant overexpression of ENO2 in PDAC tissues,and its expression was correlated with metastasis and poor prognosis in PDAC patients.K394 was identified as a major acetylation site in ENO2 that regulates its enzymatic activity,cell metabolism and PDAC progression.Knockdown of ENO2 suppressed tumor growth and liver metastasis in PDAC.Re-expression of wild-type(WT)ENO2,but not the K394 acetylation mimetic mutant,could reverse the decreased tumor malignancy.We further characterized histone deacetylase 3(HDAC3)and P300/CBP-associated factor(PCAF)as the potential deacetylase and acetyltransferase for ENO2,respectively.HDAC3-mediated deacetylation was shown to lead to ENO2 activation and enhancement of glycolysis.Importantly,insulin-like growth factor-1(IGF-1)was found to decrease K394 acetylation and stimulate ENO2 activity in a dose-and time-dependent manner.The PI3K/AKT/mTOR pathway facilitated the phosphorylation of HDAC3 on S424,which promoted K394 deacetylation and activation of ENO2.Linsitinib,an oral small-molecule inhibitor of IGF-1R,could inhibit IGF-1-induced ENO2 deacetylation by HDAC3 and the PI3K/AKT/mTOR pathway.Furthermore,linsitinib showed a different effect on the growth and metastasis of PDAC depending on the overexpression of WT versus K394-mutant ENO2.Our results reveal a novel mechanism by which acetylation negatively regulates ENO2 activity in the metastasis of PDAC by modulating glycolysis.Blockade of IGF-1-induced ENO2 deacetylation represents a promising strategy to prevent the development of PDAC. | Yan Zheng Chao Wu Jimeng Yang Yue Zhao Huliang Jia Min Xue Da Xu Feng Yang Deliang Fu Chaoqun Wang Beiyuan Hu Ze Zhang Tianen Li Shican Yan Xuan Wang Peter J.Nelson Christiane Bruns Lunxiu Qin Qiongzhu Dong | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 3 |
| 3 | A Prognostic Nomogram for Hepatocellular Carcinoma Based on Wound Healing and Immune Checkpoint Genes显示文摘Background and Aims:Wound healing and tumor progression share some common biological features;however,how variations in wound healing patterns affect hepatocellular carcinoma(HCC)prognosis remains unclear.Methods:We analyzed the wound healing patterns of 594 HCC samples from The Cancer Genome Atlas(TCGA)and the International Cancer Genome Consortium(ICGC)and correlated them with immune infiltration and the expression levels of immune checkpoint genes.A risk score,which we named the“heal.immune”score,was established via stepwise Cox estimation.We constructed a nomogram based on age,sex,TNM stage,and heal.immune score and explored its predictive value for HCC prognosis.Seventy-four clinical patients were enrolled in this study,and all were from Huashan Hospital of Fudan University between 2015 and 2017 to serve as an independent validation group.Results:We identified two distinct wound healing patterns in HCC.The biological processes of healing cluster 1(C1)are related to metabolism,while those of healing cluster 2(C2)are related to the inflammatory response and immune cell accumulation.A total of 565 wound healing-related genes(based on Gene Ontology)and 25 immune checkpoint genes were considered.By analyzing differentially expressed genes and implementing a stepwise Cox estimation analysis,six genes with p values less than 0.02 in a multivariate Cox estimation were chosen as the“heal.immune”gene set(FCER1G,PLAT,ITGA5,CCNB1,CD86 and CD40).The“heal.immune”gene set,as an OS risk factor,was further validated in Fudan cohort.We constructed a nomogram to predict the 1-,3-and 5-year overall survival(OS)in the TCGA cohort.The area under curve vales of the receiver characteristic operator curves were 0.82,0.76 and 0.73 in the training group and 0.84,0.76 and 0.72 in the test group.Conclusions:We established a prognostic nomogram based on the heal.immune gene signature,which includes six wound healing-and immunity-related genes.This nomogram accurately predicts the OS of HCC patients. | Beiyuan Hu Xiaotian Shen Wei Qin Lan Zhang Tiantian Zou Qiongzhu Dong Lun-Xiu Qin | 2022 | Journal of Clinical and Translational Hepatology2022，10，5： | 1 |
| 4 | Exosomal S100A4 derived from highly metastatic hepatocellular carcinoma cells promotes metastasis by activating STAT3显示文摘Intercellular cross-talk plays important roles in cancer progression and metastasis.Yet how these cancer cells interact with each other is still largely unknown.Exosomes released by tumor cells have been proved to be effective cell-to-cell signal mediators.We explored the functional roles of exosomes in metastasis and the potential prognostic values for hepatocellular carcinoma(HCC).Exosomes were extracted from HCC cells of different metastatic potentials.The metastatic effects of exosomes derived from highly metastatic HCC cells(HMH)were evaluated both in vitro and in vivo.Exosomal proteins were identified with iTRAQ mass spectrum and verified in cell lines,xenograft tumor samples,and functional analyses.Exosomes released by HMH significantly enhanced the in vitro invasion and in vivo metastasis of low metastatic HCC cells(LMH).S100 calcium-binding protein A4(S100A4)was identified as a functional factor in exosomes derived from HMH.S100A4r,ch exosomes significantly promoted tumor metastasis both in vitro and in vivo compared with S100A4^(rich) exosomes or controls.Moreover,exosomal S100A4 could induce expression of osteopontin(OPN),along with other tumor metastasis/stemness-related genes.Exosomal S100A4 activated OPN transcription via STAT3 phosphorylation.HCC patients with high exosomal S100A4 in plasma also had a poorer prognosis.In conclusion,exosomes from HMH could promote the metastatic potential of LMH,and exosomal S100A4 is a key enhancer for HCC metastasis,activating STAT3 phosphorylation and up-regulating OPN expression.This suggested exosomal S100A4 to be a novel prognostic marker and therapeutic target for HCC metastasis. | Haoting Sun Chaoqun Wang Beiyuan Hu Xiaomei Gao Tiantian Zou Qin Luo Mo Chen Yan Fu Yuanyuan Sheng Kaili Zhang Yan Zheng Xudong Ren Shican Yan Yan Geng Luyu Yang Qiongzhu Dong Lunxiu Qin | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 1 |
| 5 | Massive Genomic Rearrangement Acquired in a Single Catastrophic Event during Cancer Development显示文摘 | Philip J. Stephens Chris D. Greenman Beiyuan Fu Fengtang Yang Graham R. Bignell Laura J. Mudie Erin D. Pleasance King Wai Lau David Beare Lucy A. Stebbings Stuart McLaren Meng-Lay Lin David J. McBride Ignacio Varela Serena Nik-Zainal Catherine Leroy Mingm | 2011 | Cell2011，，1： | 1 |
| 6 | Heavy metal pollution increases soil microbial carbon limitation:Evidence from ecological enzyme stoichiometry显示文摘Heavy metals can exist in soil for a long time and seriously affect soil quality.The coexistence of various heavy metal pollutants leads to biotoxicity and alters the activity of microorganisms.Soil microbial metabolism plays an important role in nutrient cycling and biochemical processes of soil ecosystem.However,the effects of heavy metal contamination on microbial metabolism in soil are still unclear.This study aims to reveal the responses of microbial metabolic limitation to heavy metals using extracellular enzyme stoichiometry,and further to evaluate the potential impacts of heavy metal pollution on soil nutrient cycle.The results showed that soil microbial metabolism reflected by the ecoenzymatic activities had a significant response to soil heavy metals pollution.The metabolism was limited by soil carbon(C)and phosphorus(P)under varied heavy metal levels,and the increase of heavy metal concentration significantly increased the microbial C limitation,while had no effect on microbial P limitation.Microorganisms may increase the energy investment in metabolism to resist heavy metal stress and thus induce C release.The results suggest that energy metabolism selected by microorganisms in response to long-term heavy metal stress could increase soil C release,which is not conducive to the soil C sequestration.Our study emphasizes that ecoenzymatic stoichiometry could be a promising methodology for evaluating the toxicity of heavy metal pollution and its ecological effects on nutrient cycling. | Mingzhe Xu Yongxing Cui Jingzi Beiyuan Xia Wang Chengjiao Duan Linchuan Fang | 2021 | Soil Ecology Letters2021，3，3： | 1 |
| 7 | A novel microfluidic flow-cytometry for counting numbers of single-cell β-actins显示文摘As a house keeping protein with stable expressions, β-actin is used as a loading control in normalization of western blotting. However, the actual numbers of β-actins at the single-cell level remain elusive. Based on a homedeveloped flow cytometry, single-cell numbers of β-actin from 8 cell types(subtypes) and 2 tumour patient samples were quantified as 9.62 ± 4.29 × 105(A549, Ncell= 14,242), 6.46 ± 3.34 × 105(Hep G2, Ncell= 35,932),1.58 ± 0.90 × 106(MCF 10 A, N6 cell= 16,650), 1.08 ± 0.48 × 10(HeLa, Ncell= 26,151), 7.60 ± 4.34 × 105(PC3, Ncell= 11,922), 1.10 ± 0.72 × 106(SACC-83, Ncell= 13,616), 8.58 ± 4.54 × 105(CAL 27, Ncell= 7271),9.00 ± 4.69 × 105(CAL 27-LN2, Ncell= 6222), 8.26 ± 4.48 × 105(Oral Tumour Patient I, Ncell= 359), and8.19 ± 5.12 × 105(Oral Tumour Patient II, Ncell= 175), and were analyzed by statistical approaches including one-way analysis of variance, neural network based pattern recognition and Bayesian estimation, with varied expressions of β-actins among different cell types located. The dataset reported in this study may serve as a reference in future studies of quantitative protein analysis. | Lixing Liu Beiyuan Fan Hongyu Yang Deyong Chen Shuang Zhang Junbo Wang Jian Chen | 2020 | Nanotechnology and Precision Engineering2020，3，3： | 0 |
| 8 | Stability of potentially toxic elements in municipal sludge biochars modified by MgCl2 and phosphate显示文摘Municipal wastewater sludge can be pyrolyzed as biochars to better use nutrients and stabilize carbon compared with other typical technologies,such as landfill and incineration.However,sludge-derived biochars might contain large amounts of potentially toxic elements(PTEs),such as Zn,Cu,Cr,Ni,Pb,and As.The stability of PTEs in biochars might be improved by higher pyrolytic temperatures,which can be further improved by different modifications.Herein,PO4-modification at 300°C and Cl-modification at 700°C were carried out,respectively,to enhance the stability of PTEs.Various leaching tests have been performed to assess the stability of PTEs in biochars,including the synthetic precipitation leaching procedure(SPLP),toxicity characteristic leaching procedure(TCLP),diethylenetriamine pentaacetate(DTPA)extraction,and in vitro simple bioaccessibility extraction test(SBET).The morphological structure,elemental mapping,and mineral formation of the pristine and modified biochars were studied by scanning electron microscopy–energy-dispersive X-ray spectroscopy(SEM–EDS)and X-ray diffraction(XRD).Our results suggested that the leachability,mobility,plant-availability,and bioaccessibility of most PTEs were decreased by pyrolysis,yet the total contents of PTEs were elevated,especially at 700°C.Generally,modification by phosphates and MgCl2 enhanced the stability of PTEs in biochars.Nevertheless,it should be noted that higher bioaccessibility of PTEs was observed in biochars of P-modification than Cl-modification,which is associated with the dissolution of phosphate precipitates under acidic conditions(pH<2).Additionally,Cl-modification leads to higher plant-available Zn and Cu and bioaccessible Zn compared with the unmodified biochar produced at 700°C. | Qiqi Huang Siqi Chen Jinhao Lin Jingzi Beiyuan Jin Wang Juan Liu Yundang Wu Xiaolian Wu Fuhua Li Wenbing Yuan Chengrong Nie | 2023 | Waste Disposal and Sustainable Energy2023，5，1： | 0 |
| 9 | Size Evolution of the Surface Short Fatigue Cracks of 1Cr18Ni9Ti Weld Metal显示文摘Size evolution of the surface short fatigue cracks of 1Cr18Ni9Ti weld metal was investigated. A local viewpoint is applied to be agreement with a so-called 'effectively short fatigue crack criterion'. Attention was paid to the dominant effectively short fatigue crack (DESFC) initiation zone and the zones ahead of the DESFC tips. The results revealed that the evolutionary size shows a significant character of microstructural short crack (MSC) and physical short crack (PSC) stages. In the MSC stage, fatigue damage is due to mainly the initiation and irregular growth of the effectively short fatigue cracks (ESFCs). In the PSC stage, the damage is conversely due to mainly the DESFC growth and partially, the growth of the ESFCs and the coalescence of the ESFCs themselves with the DESFC. The process involves from a non-ordered/chaotic state in the initiation of MSC stage, gradually to an independently random state at the transition point between the MSC and PSC stages and then, to an ordered/history-dependent random state. Interactive effect of the collective cracks is stronger and shows an increase in the MSC stage. It reaches a maximum value at the transition point and then, tends to a decrease in the PSC stage. The DESFC acts as a result of the interactive cracks and thus, is deemed suitable to describe the behaviour of collective cracks. | Yongxiang ZHAOInstitute of Mechanical Engineering, Southwest Jiaotong University, Beiyuan 32-2-06#, Chengdu 610031, China | 2003 | Journal of Materials Science & Technology2003，19，2： | 0 |
| 10 | Structure and Catalytic Behaviour of Saponite and Its Cross-Linking Product显示文摘Synthetic saponites have been intercalated with hydroxyl aluminium oligomers and characterized with several techniques. The basal 001 peak of the pillared saponite (PS) is 1. 8 nm. The amount of the aluminium in the tctrahedral sheet is correlative with cross-linking density. The sheet-to-pillar linkage mode may be Si-O-AlpⅥ. The acid sites in pillared saponite may locate either at surface of clay or at that of pillar. The cumene conversion is relative to accessible acid sites on the surface of PS. The pillar density in the PS has an effect on the pore structure which is correlative with shape selectivity during the reaction between ethanol and ammonia. | Jiang Dazhen, Liu Ziyang, Sun Tie and Ren Beiyuan (Department of Chemistry, Jilin University, Changchun)Min Enze and He Mingyuan (Research Institute of Petroleum Processing, Beijing) | 1991 | Chemical Research in Chinese Universities1991，7，3： | 0 |
| 11 | Comparative Analysis on Physical Quantity Diagnosis and Wind Profile Radar Data of Two Heavy Rainfalls显示文摘[Objective] The research aimed to contrast physical quantity diagnosis and wind profile radar data of two heavy rainfalls. [Method] From circulation background,physical quantity field and wind profile radar data,we analyzed two big rainstorm weather processes ( 8-9 July and August 10) in Lianyungang City in 2012. [Result] Rainstorm generation was related to favorable large-scale circulation situation. The first-stage precipitation during 8-9 July was warm-zone precipitation,and the precipitation at the second stage was triggered by shear line. Precipitation on August 10 was generated by typhoon low-pressure inverted trough and cold air. Sufficient water vapor content and strong water vapor transportation were favorable for generation of the heavy precipitation. Suction effect by divergence at high layer and convergence at middle and low layers was favorable for maintenance of the strong ascending motion. Occurrence of the heavy precipitation must have ascending motion condition. But it was not that the stronger the ascending motion,the stronger the rainfall intensity. K index and θse 500-θse 850 were closely related to rainstorm occurrence. Horizontal wind data of the wind profile radar provided fine structure of the atmospheric horizontal motion at vertical direction,could clearly display vertical structure of the wind field in rainstorm process,and directly reflected change characteristics of the wind field in precipitation process. [Conclusion] The research could provide reference for future forecast work. | Yunfeng Zhu Mengxi Yang Jie Liu Beiyuan Wang Haiwei Duo | 2013 | Meteorological and Environmental Research2013，4，5： | 0 |
| 12 | The m6A methylation landscape stratifies hepatocellular carcinoma into 3 subtypes with distinct metabolic characteristics显示文摘Objective:Epigenetic aberration plays an important role in the development and progression of hepatocellular carcinoma(HCC).However,the alteration of RNA N6-methyladenosine(m6A)modifications and its role in HCC progression remain unclear.We therefore aimed to provide evidence using bioinformatics analysis.Methods:We comprehensively analyzed the m6A regulator modification patterns of 605 HCC samples and correlated them with metabolic alteration characteristics.We elucidated 390 gene-based m6A-related signatures and defined an m6Ascore to quantify m6A modifications.We then assessed their values for predicting prognoses and therapeutic responses in HCC patients.Results:We identified 3 distinct m6A modification patterns in HCC,and each pattern had distinct metabolic characteristics.The evaluation of m6A modification patterns using m6Ascores could predict the prognoses,tumor stages,and responses to sorafenib treatments of HCC patients.A nomogram based on m6Ascores showed high accuracy in predicting the overall survival of patients.The area under the receiver operating characteristic curve of predictions of 1,3,and 5-year overall survivals were 0.71,0.69,and 0.70 in the training cohort,and in the test cohort it was 0.74,0.75,and 0.71,respectively.M6Acluster C1,which corresponded to hypoactive mRNA methylation,lower expression of m6A regulators,and a lower m6Ascore,was characterized by metabolic hyperactivity,lower tumor stage,better prognosis,and lower response to sorafenib treatment.In contrast,m6Acluster C3 was distinct in its hyperactive mRNA methylations,higher expression of m6A regulators,and higher m6Ascores,and was characterized by hypoactive metabolism,advanced tumor stage,poorer prognosis,and a better response to sorafenib.The m6Acluster,C2,was intermediate between C1 and C3.Conclusions:HCCs harbored distinct m6A regulator modification patterns that contributed to the metabolic heterogeneity and diversity of HCC.Development of m6A gene signatures and the m6Ascore provides a more comprehensive understanding of m6A modifications in HCC,and helps predict the prognosis and treatment response. | Xiaotian Shen Beiyuan Hu Jing Xu Wei Qin Yan Fu Shun Wang Qiongzhu Dong Lunxiu Qin | 2020 | Cancer Biology & Medicine2020，17，4： | 0 |
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