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| 1 | 基质辅助激光解吸电离飞行时间质谱技术在临床微生物鉴定中的应用显示文摘近年来,基质辅助激光解吸电离飞行时间质谱技术(MALDI—TOF—MS)已从物理实验室走进临床微生物实验室,用于微生物的鉴定,正逐步取代由Pasteur和Koch建立的基于细菌表型的传统鉴定方法。许多欧洲微生物实验室目前正在使用MALDI-TOF-MS,大大提高了工作效率。鉴于美国食品药品管理局已经批准其用于体外诊断,MALDI-TOF-MS将很快在美国普及应用。这也将进一步推动了质谱技术在微生物药敏和流行学分型中的发展和应用。本文综述了MALDI—TOF—MS在临床微生物实验室的应用现状,并通过多中心临床试验结果,比较不同MALDI—TOF-MS仪器获得的诊断数据,重点阐述其在临床微生物领域的相关研究和应用。 | Marc van Nuenen Kirk Doing 顾兵 Alex van Belkum | 2015 | 中华检验医学杂志2015，38，1： | 11 |
| 2 | Improved diagnosis of Trichomonas vaginalis infection by PCR using vaginal swabs and urine specimens compared to diagnosis by wet mount microscopy,culture,and fluorescent staining显示文摘 | Van Der Schee.C A.van Belkum L.Zwijgers L | | 0，，12： | 1 |
| 3 | A clinical study on the association of Trichomonas vaginalis and Mycoplasma hominis infections in women attending a sexually transmitted disease (STD) outpatient clinic 显示文摘 | van Belkum A van der Schee C van der Meijden WI | 2001 | FEMS Immunol Med Microbiol2001，32，1： | 1 |
| 4 | Colonisation by Streptococcus pneumoniae and Staphylococcus aureus in healthy children显示文摘 | Bogaert D van Belkum A Sluijter M | 2004 | The Lancet2004，363，9424： | 1 |
| 5 | Comparison of microscopy, real-time PCR and a rapid immunoassay for the de- tection of Giardia larnblia in human stool specimens4 显示文摘 | Schuurman T Lankamp P van Belkum A et ol | 2007 | Clin Microbiol Infect2007，13，12： | 1 |
| 6 | Molecular typing methods for epidemiological studies in medical microbiology显示文摘 | | 1996 | Med Microbiol Lett1996，5，： | 1 |
| 7 | Development of a species-specific polymerase Chain reaction assay for Gardnerella vaginalis显示文摘 | Belkum AV Koeken A Vandamme R | 1995 | Molecul Cell Probes1995，9，3： | 1 |
| 8 | Comparison of eight methods to detect vancomycin resistance in enterococci 显示文摘 | Endtz HP Van Den Braak N Van Belkum A | 1998 | J Clin Microbiol1998，36，2： | 1 |
| 9 | Discrimination ofAspergiUus len- tulus from Aspergillus fumigatus by Raman spectroscopy and MALDI-TOF MS 显示文摘 | Verwer P E van Leeuwen W B Girard V Monnin V van Belkum A Staab J F | 2014 | European journal of clinical microbiology & infectious diseases: official publication of the European Society of Clinical Microbiology2014，33，2： | 1 |
| 10 | Short sequence repeats in microbial pathogenesis and evolution显示文摘 | Van Belkum A | 1999 | Cell Mol Life Sci1999，56，910： | 1 |
| 11 | FEMS tracing isolates of bacterial species by multilocus variable number of tandem repeat analysis 显示文摘 | Belkum A | 2007 | Immunol Med Microbiol2007，49，： | 1 |
| 12 | Improved diagnosis of Trichomonas Vaginalis infection by PCR using vaginalswabs and urine specimens compared to diagnosis by wet mount microscopy, culture, and fluorescent staining 显示文摘 | van Der Schee C van Belkum A Zwijgers L | 1999 | J Clin MICrobiol1999，37，12： | 1 |
| 13 | Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks 显示文摘 | Kluytmans J van Belkum A Verbrugh H | 1997 | Clin Microbiol Rev1997，10，3： | 1 |
| 14 | Development of a species-specific polymerase chain reaction assay for Gardnerella vaginalis显示文摘 | Belkum AV Koeken A Vandamme P | 1995 | Molecul cel probes1995，9，： | 1 |
| 15 | Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks 显示文摘 | Kluytmans J van Belkum A Verbrugh H | 1997 | Clin Microbiol Rev1997，10，3： | 1 |
| 16 | Are variable repeats in the spa gene suitable targets for epidemiological studies of methicillin-resistant Staphylococcus aureus strains 显示文摘 | Van Belkum A Riewerts Eriksen N Sijmons M | 1996 | Eur J Clin Microbiol Infect Dis1996，15，9： | 1 |
| 17 | Performance of CHROMagar MRSA medium for detection of methieillin-resistant Staphylococcus aureus 显示文摘 | DIEDEREN B VAN DUIJN I VAN BELKUM A | 2005 | J Clin Microbiol2005，43，3： | 1 |
| 18 | Novel BOX repeat PCR assay for high-resolution typing of Streptococcus pneumoniae stains 显示文摘 | VAN BELKUM A SLUIJUTER M DE GROOT R | 1996 | J C lin Microbiol1996，34，： | 1 |
| 19 | Patient-to-patientspread of a single strain of corynebacterium striatum causing in-fections in a surgical intensive care unit显示文摘 | Brandenburg AH Belkum A Pelt C | 1996 | Clin Microbiol1996，34，9： | 1 |
| 20 | Development of a species-specific polymerase chain reaction for Gardnerella vaginalis显示文摘 | Belkum AV Koeken A Vandamme P | 1995 | Mol Cell Probes1995，9，3： | 1 |
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