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| 1 | Chemical constituents, cytotoxic, antifungal and antimicrobial properties of Centaurea diluta Ait. subsp. algeriensis(Coss. & Dur.) Maire显示文摘Objective:To investigate the chemical composition of a moderately polar extract(CHC1_3 soluble part of the MeOH-H_2O extract) obtained from the aerial parts(leaves and flowers) of Centaurea diluta Ait.subsp.algeriensis(Coss.& Dur.) Maire,a species endemic to Algeria and Morocco on which no reports are available to date.To evaluate in vitro the cytotoxic,antifungal and antimicrobial activities of this extract and the cytotoxic and antimicrobial activities of its isolated secondary metabolites.Methods:The cytotoxic effects of the extract were investigated on 3 human cancer cell lines i.e.the A549 non-small-cell lung carcinoma(NSCLC),the MCF7 breast adenocarcinoma and the U373 glioblastoma using a MTT colorimetric assay.Biological data allowed to guide the fractionation of the extract by separation and purification on silica gel 60(CC and TLC).The isolated compounds which were characterized by spectral analysis,mainly HR-ESIMS,HR-EIMS,UV and NMR experiments(~1H,^(13)C,COSY,ROESY,HSQC and HMBC) and comparison of their spectroscopic data with those reported in the literature,were evaluated for cytotoxic activities on six cancer cell lines(A549,MCF7,U373,Hs683 human glioma,PC3 human prostate and B16-F10 murine melanoma).The direct and indirect antibacterial and antifungal activities were determined using microdilution methods for the raw extract and TLC-bioautography and microdilution methods against standard and clinical strains for the isolated compounds.Results:The raw extract reduced cell viability with IC_(50)s of 27,25 and 21 μg/mL on A549,MCF7 and U373,respectively.Five secondary metabolites:two phenolic compounds(vanillin 1,paridol 3),a lignan[(-)-arctigenin 2]and two flavonoid aglycones(eupatilin 4 and jaceosidin 5),were then isolated from this extract.Moderate cytotoxic effects were observed for(-)-arctigenin 2(IC_(50)s:28 and 33μM on Hs683 and B16-F10,respectively),eupatilin 4(IC_(50)s:33 and 47 μM on B16-F10 and PC3,respectively) and jaceosidin 5(IC_(50)s:32 and 40 μM on PC3 and B16-F10,respectively).Conclusions:All the isolated compounds were described for the first time from this species.Although inactive against 7 tested microorganisms(fungi,bacteria and yeast,human or plant pathogens),the raw extract was able to potentiate the effect of beta-lactam antibiotics on methicillin-resistant Staphylococcus aureus(MRSA),reducing the minimal inhibitory concentrations(MICs) by a factor of 2-32-fold.No synergy was found between the extract and streptomycin.From the five isolated compounds only jaseosidin 5 showed a moderate antimicrobial activity. | Hanene Zater Joelle Huet Veronique Fontaine Samir Benayache Caroline Stevigny Pierre Duez Fadila Benayache | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，6： | 2 |
| 2 | Terpenoids and triynepoxide from the aerial part of Rhantherium adpressum显示文摘 | M. Bouheroum S. Benayache F. Benayache L. Zaiter J. M. Barrera L. Francisco | 2007 | Chemistry of Natural Compounds2007，，1： | 1 |
| 3 | Isolation from Eucalyptus occidentalis and identification of a new kaempferol derivative that induces apoptosis in human myeloid leukemia cells显示文摘 | Benyahia S Benayache S Benayache F | 2004 | J Nat Prod2004，67，4： | 1 |
| 4 | Ontology-Based Organizational Memory for e-Learning显示文摘 | Abel M.-H Benayache A Lenne D | | 0，，04： | 1 |
| 5 | A flavonoid with cytotoxic activity and other constituents from Centaurea africana显示文摘 | Ramdane Seghiri Ouahiba Boumaza Ratiba Mekkiou Samir Benayache Paul Mosset José Quintana Francisco Estévez Francisco León Jaime Bermejo Fadila Benayache | 2009 | Phytochemistry Letters2009，，： | 1 |
| 6 | Flavonoid aglycones from Centaurea napifolia 显示文摘 | AKKAL S BENAYACHE F BENTAMENE A | 2003 | Chem Nat Compd2003，39，2： | 1 |
| 7 | Flavonoids from Chrysanthemum fuscatum显示文摘 | S. Ameddah H. Dendougui A. Menad R. Mekkiou Z. Meraihi S. Benayache F. Benayache | 2007 | Chemistry of Natural Compounds2007，，2： | 1 |
| 8 | Sesquiterpene lactones from Centaurea musimomum . Antiplasmodial and cytotoxic activities显示文摘 | Kamel Medjroubi Fadila Benayache Jaime Bermejo | 2005 | Fitoterapia2005，，： | 1 |
| 9 | Flavonoids of Serratula cichoracea and their antioxidant activity显示文摘 | L. Aliouche H. Zater D. Zama A. Bentamene R. Seghiri R. Mekkiou S. Benayache F. Benayache | 2007 | Chemistry of Natural Compounds2007，，： | 1 |
| 10 | A new guaianolide and other constituents from Achillea ligustica显示文摘 | Azzedine Boudjerda Hanene Zater Samir Benayache Jean-Claude Chalchat Javier González-Platas Francisco León Ignacio Brouard Jaime Bermejo Fadila Benayache | 2007 | Biochemical Systematics and Ecology2007，，： | 1 |
| 11 | Flavonoids from lnula viscosa L 显示文摘 | Benayache S Benayache F Dendougui H | 1991 | Plant Med et Phytotherapie1991，25，4： | 1 |
| 12 | Study of Variation in Phosphocalcic Metabolism during Pregnancy and Postpartum in the Holstein Cow of Constantine Region显示文摘Pregnancy, parturition, and lactation represent physiological changes of organism, which activates adaptation mechanismsdedicated to maintain homeostasis during peripartum period. Despite of the appropriate input of calcium and phosphorus, thehomeostasis of phosphocalcic metabolism is sometimes faulty, in particular among animals with high production potential. The aim ofthis study is to define usual values of calcium and phosphorus during pregnancy and postpartum period. This paraclinic tool will serveto a good interpretation when used by veterinarians. A second goal is to study the influence of physiological stage on phosphocalcicmetabolism in milch cow. The hereby study was conducted on 17 Prim' Holsteins cow breed from 3 to 5 years old, clinically healthy,multiparous and derived from two dairy farms located in the wilaya of Constantine. Blood samples were taken from the jugular veinusing dry tubes at different physiological stages (early gestation, mid gestation, late gestation, pre-partum, one day after parturition, andone month postpartum). All of the samples correctly identified were transported to a specialized laboratory where specific kits wereused. Blood calcium and phosphorus levels have significantly varied depending on physiological stage (p 〈 0.01). They showed a highpre-partum variation, with a rate of 94.71 mg/mL and 61.99 mg/mL, respectively. These results can be used as means of follow-upreproduction for a better management of dairy fanning. | Selma Benhizia Mohamed Amir Boudhene Sara Benayache Nadjoua Arzour Abdeslam Mekroud | 2017 | Journal of Pharmacy and Pharmacology2017，5，8： | 0 |
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