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| 1 | Partial Beclin 1 silencing aggravates doxorubicin-and Fasinduced apoptosis in HepG2 cells显示文摘瞄准:在 HepG2 房间的危险性上调查 Beclin 1 的角色在反船边交货抗体或 doxorubicin 治疗以后经历 apoptosis。方法:Beclin 1 silencing 用 RNA 干扰被完成。DNA 倍性, apoptotic 房间的百分比和 mitochondrial 膜电位被流动血细胞计数估计。多形核白细胞(自动数据处理核糖) 的 Beclin 1, Bcl-X (L) 和细胞色素 c,和劈开聚合酶(PARP ) 的层次是由使用西方的污点的 assayed。结果:Beclin 1 表示在 Beclin 1 siRNA transfection 以后由 75% 72 h 减少了。部分 Beclin 1 silencing 显著地增加了 subG1 房间的百分比有 doxorubicin 或反船边交货抗体的 24 和 40 h 术后疗法,分别地并且这 potentiation 被处理与一个 pan-caspase 禁止者废除。部分 Beclin 1 silencing 也增加了 PARP 劈开, mitochondrial 膜去极和 cytosolic 细胞色素 c。部分 Beclin 1 silencing 的 pro-apoptotic 后果没在 Bcl-X (L) 表示与衰落被联系。结论:部分 Beclin 1 silencing 在与反船边交货抗体或与 doxorubicin 对待的 HepG2 房间加重 mitochondrial permeabilization 和 apoptosis。 | Fanny Daniel Agnès Legrand Dominique Pessayre Nathalie Vadrot Véronique Descatoire Dominique Bernuau | 2006 | World Journal of Gastroenterology2006，12，18： | 11 |
| 2 | c-Fos overexpression increases the proliferation of human hepatocytes by stabilizing nuclear Cyclin D1显示文摘AIM: To investigate the effect of stable c-Fos overexpression on immortalized human hepatocyte (IHH) proliferation. METHODS: IHHs stably transfected with c-Fos (IHH-Fos) or an empty vector (IHH-C) were grown in medium supplemented with 1% serum or stimulated with 10% serum. Cell proliferation was assessed by cell counts, 3H-thymidine uptake and fl ow cytometry analyses. The levels of cell cycle regulatory proteins (Cyclin D1, E, A) cyclin dependent kinases (cdk) cdk2, cdk4, cdk6, and their inhibitors p15, p16, p21, p27, to- tal and phosphorylated GSK-3β and epidermal growth factor receptor (EGF-R) were assayed by Western blot- ting. Analysis of Cyclin D1 mRNA levels was performed by reverse transcription-polymerase chain reaction and real-time polymerase chain reaction (PCR) analysis. Stability of Cyclin D1 was studied by cycloheximide blockade experiments. RESULTS: Stable c-Fos overexpression increased cell proliferation under low serum conditions and resulted in a two-fold increase in [3H]-thymidine incorpora- tion following serum addition. Cell cycle analysis by fl ow cytometry showed that c-Fos accelerated the cell cycle kinetics. Following serum stimulation, Cyclin D1 was more abundantly expressed in c-Fos overexpressing cells. Cyclin D1 accumulation did not result from increased transcriptional activation, but from nuclear stabilization. Overexpression of c-Fos correlated with higher nuclear levels of inactive phosphorylated GSK- 3β, a kinase involved in Cyclin D1 degradation and higher levels of EGF-R mRNA, and EGF-R protein compared to IHH-C both in serum starved, and in serum stimulated cells. Abrogation of EGF-R signalling in IHH- Fos by treatment with AG1478, a specif ic EGF-R tyrosine kinase inhibitor, prevented the phosphorylation of GSK-3β induced by serum stimulation and decreased Cyclin D1 stability in the nucleus. CONCLUSION: Our results clearly indicate a positive role for c-Fos in cell cycle regulation in hepatocytes. Importantly, we delineate a new mechanism by which c-Fos could contribute to hepatocarcinogenesis through stabilization of Cyclin D1 within the nucleus, evoking a new feature to c-Fos implication in hepatocellular carcinoma. | Meryem Güller Kahina Toualbi-Abed Agnès Legrand Laurence Michel Alain Mauviel Dominique Bernuau Fanny Daniel | 2008 | World Journal of Gastroenterology2008，14，41： | 6 |
| 3 | 肝硬化组织中C-H-ras、C-fos、C-jun和P^(53)基因的表达显示文摘用原位杂交及免疫组化的方法检测11例硬化肝脏、7例正常肝脏中 C-H-ras、C-fos、C-jun 和 p^(53)基因的表达。结果,正常肝脏均为阴性;10例(91%)肝硬化中 C-H-ras 基因异常表达;5例检出 C-fos 基因的异常表达;1例检出 C-jun 基因表达;未检出突变型 P^(53)蛋白在硬化肝脏中的表达。提示,C-H—ras 和 C-fos 基因在肝硬化组织中的异常表达构成肝癌发生早期事件中基因异常的一部分。 | 刘平 吕秀珍 Dominique Bernuau | 1996 | 南京医科大学学报(自然科学版)1996，16，2： | 2 |
| 4 | Criteria for emergency liver transplantation in patients with acute viral hepatitis and factor Ⅴ below 50% of: a prospective study 显示文摘 | Bernuau J Goudeau A Durand F | 1991 | Hepatology1991，14，4： | 1 |
| 5 | Acute liver failure:avoidance of deleterious cofactors and early specific medical therapy for the liver are better than late intensive care for the brain显示文摘 | Bernuau J | | 0，，： | 1 |
| 6 | Acute liver failure: avoidance of deleterious cofactors and early specific medical therapy for the liver are better than late intensive care for the brain显示文摘 | Jacques Bernuau | 2004 | Journal of Hepatology2004，，1： | 1 |
| 7 | Fulminant hepatic failure due to transient circulatory failure in patients with chronic heart disease显示文摘 | O. Nouel MD Dr. J. Henrion MD J. Bernuau MD C. Degott MD B. Rueff MD Dr. J. -P. Benhamou MD | 1980 | Digestive Diseases and Sciences1980，，1： | 1 |
| 8 | Fulminant and subfulminant liver failure: definitions and causes 显示文摘 | BERNUAU J RUEFF B BENHAMOU JP | 1986 | Semin Liver Dis1986，6，： | 1 |
| 9 | WHV/myc转基因小鼠肝癌发生过程中c-jun,c-fos和c-H-ras基因的表达显示文摘用核酸分子原位杂交的方法,检测了WHV/myc转基因小鼠肝癌发生过程中c-jun、c-fos和c-H-ras基因的表达情况。正常小鼠(10天龄和4月龄)肝脏中可检测出上述三种基因呈低水平的表达。10天龄转基因小鼠肝脏中这三种基因的表达与正常同龄小鼠相似,而4月龄转基因小鼠肝脏中三种基因的表达强度明显高于正常小鼠,且杂交信号的分布范围广。肿瘤形成阶段,仍可在肝肿瘤结节中检测到这三种癌基因较弱的表达信号。结果表明,在WHV/myc转基因小鼠肝肿瘤发生前c-myc转染基因表达的“沉默”期,存在一些癌基因在肝脏中异常表达的现象。 | 刘平 Dominique Bernuau Gerard Feldmann | 1997 | 南京医科大学学报(自然科学版)1997，17，4： | 1 |
| 10 | Primary biliary cirrhosis and pregnancy 显示文摘 | Ducarme G Bernuau J Luton D | 2014 | J Gynecol Obstet Biol Reprod (Paris)2014，43，： | 1 |
| 11 | Keratinocyte growth factor expression by fibroblasts in pulmonary fibrosis: poor response to interleukin-lbeta显示文摘 | Marchand-Adam S Plantier L Bernuau D | 2005 | Am J Respir Cell Mol Biol2005，32，5： | 1 |
| 12 | Multivariate analysis of prognosit ic factors in fulminant hepatitis B显示文摘 | Bernuau J Goudeau A Doynard T | 1986 | Hepatology1986，6，4： | 1 |
| 13 | Induction of cerebral hyperemia by ammonia plus endotoxin:does hyperammonemia unlock the blood-brain barrier显示文摘 | Jalan R Bernuau J | | 0，，02： | 1 |
| 14 | Decrease in serum ALT and increase in serum HCV RNA during pregnancy in women with chronic hepatitis C显示文摘 | Gervais A Bacq Y Bernuau J | 2000 | J Hepatol2000，32，： | 1 |
| 15 | Induction of cerebral hyperemia by ammonia plus endotoxin:Does hyperammonemia unlock the blood-brain barrier?显示文摘 | Jalan R Bernuau J | 2007 | Journal of Hepatology2007，47，2： | 1 |
| 16 | Induction of cerebral hyperemia by ammonia plus endotoxin:Does hyperammonemia unlock the blood-brain barrier?显示文摘 | Jalan R Bernuau J | 2007 | Journal of Hepatology2007，47，2： | 1 |
| 17 | Wilson's disease with severehepaticin sufficiency:beneficialeffects of early administra-tion of D-penicillamine显示文摘 | Durand F Bernuau J Giostra E | 2001 | Gut2001，48，6： | 1 |
| 18 | Fulminant hepatic failure due to transient circulatory failure in patients with chronic heart disease显示文摘 | Nouel O Henrion J Bernuau J | 1980 | Dig Dis Sci1980，25，： | 1 |
| 19 | Fuminant and subfulmant liver failure: Definition and causes 显示文摘 | Bernuau J Rueff B Benhamou JP | 1986 | Sem Liv Dis1986，6，： | 1 |
| 20 | Induction of cerebral hyperemia by ammoniaplus endotoxin:Does hyperammonemia unlock the blood–brainbarrier显示文摘 | Jalan R Bernuau J | 2007 | Journal of Hepatology2007，47，2： | 1 |
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