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| 1 | Vascular involvement in diabetic subjects with ischemic foot ulcer: a new morphologic categorization of disease severity 显示文摘 | Graziani L Silvestro A Bertone V | 2007 | Eur J Vasc Endovasc Surg2007，33，10： | 1 |
| 2 | Percutaneous transluminal angioplasty is feasible and effective in patients on chronic dialysis with severe peripheral artery disease显示文摘 | Graziani L Silvestro A Bertone V | 2007 | Nephrol Dial Transplant2007，22，4： | 1 |
| 3 | A lost-wax approach to monodisperse colloids and their crystals显示文摘 | JIANG P BERTONE J F COLVIN V L | 2001 | Science2001，291，19： | 1 |
| 4 | Effects of the omega-3 fatty acid, a-linolenic acid, on lipopolysaccharide- challenged synovial explants from horses显示文摘 | Munsterman A S Bertone A L Zachos T A | 2005 | American Journal of Veterinary Research2005，66，9： | 1 |
| 5 | Percutaneous transluminal angioplasty is feasible and effective in patients on chronic dialysis with servere peripheral artery disease 显示文摘 | Graziani L Silvestro A Bertone V | 2007 | Nephrol Dial Tansplant2007，22，4： | 1 |
| 6 | Vascular involvement in diabetic subjects with ischemic foot ulcer:a new morphologic categorization of disease severity显示文摘 | Graziani L Silvestro A Bertone V | | 0，，： | 1 |
| 7 | Executive pay:A special report显示文摘 | Markels A Berton L | 1996 | Wall Street Journal1996，4，1： | 1 |
| 8 | Vascular involvement in diabetic subjects with ischemic foot ulcer:a new morphologic categorization of disease severity 显示文摘 | Graziani L Silvestro A Bertone V | 2007 | Eur J Vasc Endovasc Surg2007，33，4： | 1 |
| 9 | Big accounting firms weed out risky clients显示文摘 | Berton L | 1995 | Wall Street Journal1995，26，7： | 1 |
| 10 | Vascular involvement in diabetic subjects with ischemic foot ulcer:a new morphologic categorization of disease severity显示文摘 | Graziani L Silvestro A Bertone V | | 0，，04： | 1 |
| 11 | Percutaneous transluminal angioplasty is feasible and effective in patients on chronic dialysis with severe peripheral artery disease 显示文摘 | Graziani L Silvestro A Bertone V | 2007 | Nephrol Dial Trans2007，22，4： | 1 |
| 12 | Vascular involvement in diabetic subjects with ischemic foot ulcer:a new morphologic categorization of disease severity显示文摘 | Graziani L Silvestro A Bertone V | 2007 | Eur J Vasc Endovasc Surg2007，33，： | 1 |
| 13 | Vascular involvement in diabetic subjects with ischemic foot ulcer: a new mnq~hologiccategorization of disease severity显示文摘 | Graziani L Silvestro A Bertone V ct al | 2007 | EurJ Vasc Endovasc Surg2007，33，4： | 1 |
| 14 | Vascular involvement in diabetic subjects with ischemic foot ulcer:a new morphologic categorization of disease severity显示文摘 | GRAZIANI L SILVESTRO A BERTONE V | 2007 | Eur J Vasc Endovasc Surg2007，33，4： | 1 |
| 15 | Response of induced bone defects in horses to collagen matrix containing the human parathyroid hormone gene显示文摘 | Backstrom K C Bertone A L Wisner E R | 2004 | Am J Vet Res2004，65，9： | 1 |
| 16 | Decreased contraction of glycated collagen lattices coincides with impaired matrix metalloproteinase production显示文摘 | Rittie L Berton A Monboisse JC | 1999 | Biochem Biophys Res Commun1999，264，2： | 1 |
| 17 | Vascular involvement in diabetic subjects with ischemic foot ulcer:a new morphologic categorization of disease severity显示文摘 | Graziani L Silvestro A Bertone V | 2007 | Eur J Vasc Endovasc Surg2007，33，4： | 1 |
| 18 | Map- ping the stability diagram of a digital ion trap (dit) mass spectrometer varying the duty cycle of the trapping rectangular waveform显示文摘 | BERTON A TRALDI P DING L | 2008 | Journal of the American Society for Mass Spectrometry2008，19，4： | 1 |
| 19 | Fetal vs adult mesenchymal stem cells achieve greater gene expression, but less osteoinduction显示文摘AIM: To investigate adenoviral transduction in mesenchymal stem cells(MSCs) and effects on stemness in vitro and function as a cell therapy in vivo.METHODS: Bone marrow-derived adult and fetal MSC were isolated from an equine source and expanded in monolayer tissue culture. Polyethylenimine(PEI)-mediated transfection of pc DNA3-e GFP or adenoviral transduction of green fluorescent protein(GFP) was evaluated in fetal MSCs. Adenoviral-mediated transduction was chosen for subsequent experiments. All experiments were carried out at least in triplicate unless otherwise noted. Outcome assessment was obtained by flow cytometry or immunohystochemistry and included transduction efficiency, cell viability, stemness(i.e., cell proliferation, osteogenic and chondrogenic cell differentiation), and quantification of GFP expression. Fetal and adult MSCs were then transduced with an adenoviral vector containing the gene for the bone morphogenic protein 2(BMP2). In vitro BMP2 expression was assessed by enzyme linked immunosorbent assay. In addition, MSC-mediated gene delivery of BMP2 was evaluated in vivo in an osteoinduction nude mouse quadriceps model. New bone formation was evaluated by microradiography and histology.RESULTS: PEI provided greater transfection and viability in fetal MSCs than other commercial chemical reagents. Adenoviral transduction efficiency was superior to PEI-mediated transfection of GFP in fetal MSCs(81.3% ± 1.3% vs 35.0% ± 1.6%, P < 0.05) and was similar in adult MSCs(78.1% ± 1.9%). Adenoviral transduction provided significantly greater expression of GFP in fetal than adult MSCs(7.4 ± 0.1 vs 4.4 ± 0.3 millions of mean fluorescence intensity units, P < 0.01) as well as significantly greater in vitro BMP2 expression(0.16 pg/cell-day vs 0.10 pg/cell-day, P < 0.01). Fraction of fetal MSC GFP positive cells decreased significantly faster than adult MSCs(1.15% ± 0.05% vs 11.4% ± 2.1% GFP positive at 2 wk post-transduction, P < 0.05). Cell proliferation and osteogenic differentiation in vitrowere not affected by Ad transduction in both fetal and adult MSCs, but fetal MSCs had reduced chondrogenic differentiation in vitro when compared to adult(P < 0.01). Chondrogenic differentiation was also significantly reduced in Ad-GFP transduced cells(P < 0.05). AdBMP2 transduced adult MSCs induced new bone formation in more thighs than Ad-BMP2 transduced fetal MSCs(83% vs 17% of the six treated thighs per group, P < 0.05) and resulted in increased femur midshaft diameter due to greater extent of periosteal new bone(1.57 ± 0.35 mm vs 1.27 ± 0.08 mm, P < 0.05).CONCLUSION: Fetal MSCs may be genetically manipulated ex vivo with adenoviral vectors. Nonetheless, the abbreviated expression of the exogenous gene may limit their applications in vivo. | Juan E Santiago-Torres Rebecca Lovasz Alicia L Bertone | 2015 | World Journal of Stem Cells2015，7，1： | 1 |
| 20 | Vascular involvement in diabetic subjects with ischemic foot ulcer:a new morphologic categorization of disease severity显示文摘 | Graziani L Silvestro A Bertone V | 2007 | Eur J Vasc Endovasc Surg2007，33，4： | 1 |
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