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| 1 | 传统DNA疫苗载体与Semliki森林病毒复制子对HIV-1 Pr55^(gag)表达与体液免疫原性的比较性研究显示文摘为探索Semliki森林病毒 (SFV)衍生的复制型DNA载体可否用于HIV疫苗的候选载体 ,对该载体与传统DNA疫苗载体对HIV - 1Pr5 5 gag的表达与体液免疫原性进行了系统比较研究。将野生型 (wtgag)及密码子改造(syngag)的HIV - 1ⅢBgag 基因分别克隆于SFVDNA载体及传统DNA疫苗载体 [pCDNA3 1(+) ],对其Pr5 5 gag细胞内表达水平、Pr5 5 gag病毒样颗粒释放、以及在BALB/c鼠的体液免疫原性进行了比较。在 2 93T、H12 99、C2C12和BHK细胞系中 ,SFV -wtgag可以Rev非依赖方式有效表达Pr5 5 gag ,而 pC -wtgag转染的细胞不能有效表达Pr5 5 gag,从而不能诱导小鼠产生免疫反应。虽然SFV质粒的细胞转化效率明显低于pCDNA载体 ,SFV -wtgag和SFV -syngag在细胞内Pr5 5 gag的表达量与 pC -syngag相似 ,而Pr5 5 gag病毒样颗粒的释放明显低于 pC -syngag。在肌内注射免疫的小鼠中 ,低剂量 (0 1和 1 0 μg)的SFV及 pCDNAgag表达质粒均未诱导出GAG特异性免疫反应。在高剂量 (10 ,30 ,10 0 μg)免疫组中 ,与SFVgag表达质粒相比 ,pC -syngag可诱导出较高水平的TH1型GAG特异性抗体。SFV -syngag较SFV -wtgag可诱导出高水平的体液免疫反应。结果提示 ,SFV衍生的复制子单独使用不能在小鼠诱导出优于传统DNA疫苗载体的HIV - 1GAG特异性? | 张健慧 Jens Wild Kurt Bieler Marcus Graf Ludwig Deml Hans Wolf PeterLiljestrm Ralf Wagner 邵一鸣 | 2002 | 病毒学报2002，18，1： | 13 |
| 2 | Overview of constitutive laws, kinematics, homogenization and multiscale methods in crystal plasticity finite-element modeling: Theory, experiments, applications显示文摘 | F. Roters P. Eisenlohr L. Hantcherli D.D. Tjahjanto T.R. Bieler D. Raabe | 2009 | Acta Materialia2009，，4： | 3 |
| 3 | Microreactor technology : A revolution for the fine chemical and pharmaceutical industries 显示文摘 | Roberge D M Ducry L Bieler N | 2005 | Chem Eng Techno12005，28，： | 1 |
| 4 | Effect of cooling rate on microstructure and mechanical properties of eutectic Sn-Ag solder joints with and without intentionally incorporated Cu6Sn5 reinforcements显示文摘 | Jeff Sigelko S. Choi K. N. Subramanian James P. Lucas T. R. Bieler | 1999 | Journal of Electronic Materials1999，，11： | 1 |
| 5 | Microreactor technology:A revolution for the fine chemical and pharmaceutical industries显示文摘 | Roberge D M Ducry L Bieler N | 2005 | Chemical Engineering&Technology2005，28，3： | 1 |
| 6 | Overview of constitutive laws, kinematics, homogenization and multiscale methods in crystal plasticity finite-element modeling: Theory, experiments, applications 显示文摘 | ROTERS F EISENLOHR P HANTCHERLI L TJAHJANTO D D BIELER T R RAABE D | 2010 | Acta Materialia2010，58，4： | 1 |
| 7 | Development of an industrial multi-injection microreactor for fast and exothermic reactions-part Ⅱ 显示文摘 | Roberge D M Bieler N Mathier M | 2008 | Chem Eng Technol2008，31，： | 1 |
| 8 | Effects of working,heat treatment,and aging on microstructural evolution and crystallographic texture of α,α′,α′′ and β phases in Ti-6Al-4V wire显示文摘 | ZENG L BIELER T R | 2005 | Materials Science and Engineering A2005，392，： | 1 |
| 9 | Influence of Sn grain size and orientation on the thermomechanical response and reliability of Pb-free solder joints显示文摘 | THOMAS R BIELER JIANG H R | | 0，，2： | 1 |
| 10 | Comparative eflqcacy and safety of albuterol sulfate Spiros inhaler and albuterol metered-dose inhaler in asthma显示文摘 | Nelson H Kemp JP Bieler S | 1999 | Chest1999，115，2： | 1 |
| 11 | Microreactor technology:A revolution for the fine chemical and pharmaceutical industries显示文摘 | Roberge D M Ducry L Bieler N | | 0，，03： | 1 |
| 12 | Characterization of the growth of intermetallic interfacial layers of Sn-Ag and Sn-Pb eutectics solders and their composite solder on Cu substrate during isothermal long-term aging显示文摘 | CHOI S BIELER T R LUCAS J P SUBRAMANIAN K N | 1999 | J Electron Mater1999，28，11： | 1 |
| 13 | Quantification of creep strain distribution in small crept lead-free in-situ composite and non-composite solder joints 显示文摘 | MCDOUGALL J CHOI S BIELER T R LUCAS J P | 2000 | Materials Science and Engineering2000，285，： | 1 |
| 14 | Covalent binding of the anticancer drug ellipticine to DNA in V79 cells transfected with human cytochrome P450 enzymes显示文摘 | Frei E Bieler C A Arlt V M | 2002 | Biochem Pharmacol2002，64，2： | 1 |
| 15 | über die katalytische reduktion aliphatischer dicarbonsuren und ihrer ester显示文摘 | GUYER A BIELER A SOMMARUGA M | | 0，，04： | 1 |
| 16 | Zoledronate inhibits endothelial cell adhesion,migration and survival through the suppression of multiple,prenylation-dependent signaling pathways显示文摘 | Hasmim M Bieler G Rüegg C | | 0，，1： | 1 |
| 17 | Evidence for the involvement of endothelial cell integrin αvβ3 in the disruption of the tumor vasculature induced by TNF and IFN-γ显示文摘 | Ruegg C Yimaz A Bieler G | 1998 | Nat Med1998，4，： | 1 |
| 18 | Microreactor technology : a revolution for the fine chemical and pharmaceutical industries?显示文摘 | Roberge D M Ducry L Bieler N | 2005 | Chem Eng Technol2005，28，3： | 1 |
| 19 | Formation and growth of interracial intermctallic layers in eutectic Sn-Ag solder and its composite solder joints 显示文摘 | CHOI S LUCAS J P SLrBRANMANIAN K N BIELER T R | 2000 | Journal of Materials Scionce:Materials in Electronics2000，11，： | 1 |
| 20 | Processing and aging characteristics of eutectic Sn-3.5Ag solder reinforced with mechanically incorporated Ni particles显示文摘 | F. Guo J. Lee S. Choi J. P. Lucas T. R. Bieler K. N. Subramanian | 2001 | Journal of Electronic Materials2001，，9： | 1 |
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