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| 1 | RNA Methylome Reveals the m^(6)A-mediated Regulation of Flavor Metabolites in Tea Leaves under Solar-withering显示文摘The epitranscriptomic mark N6-methyladenosine(m^(6)A),which is the predominant internal modification in RNA,is important for plant responses to diverse stresses.Multiple environmental stresses caused by the tea-withering process can greatly influence the accumulation of specialized metabolites and the formation of tea flavor.However,the effects of the m^(6)A-mediated regulatory mechanism on flavor-related metabolic pathways in tea leaves remain relatively uncharacterized.We performed an integrated RNA methylome and transcriptome analysis to explore the m^(6)Amediated regulatory mechanism and its effects on flavonoid and terpenoid metabolism in tea(Camellia sinensis)leaves under solar-withering conditions.Dynamic changes in global m^(6)A level in tea leaves were mainly controlled by two m^(6)A erasers(CsALKBH4A and CsALKBH4B)during solar-withering treatments.Differentially methylated peak-associated genes following solarwithering treatments with different shading rates were assigned to terpenoid biosynthesis and spliceosome pathways.Further analyses indicated that CsALKBH4-driven RNA demethylation can directly affect the accumulation of volatile terpenoids by mediating the stability and abundance of terpenoid biosynthesis-related transcripts and also indirectly influence the flavonoid,catechin,and theaflavin contents by triggering alternative splicing-mediated regulation.Our findings revealed a novel layer of epitranscriptomic gene regulation in tea flavor-related metabolic pathways and established a link between the m^(6)A-mediated regulatory mechanism and the formation of tea flavor under solar-withering conditions. | Chen Zhu Shuting Zhang Chengzhe Zhou Caiyun Tian Biying Shi Kai Xu Linjie Huang Yun Sun Yuling Lin Zhongxiong Lai Yuqiong Guo | 2023 | Genomics, Proteomics & Bioinformatics2023，21，4： | 2 |
| 2 | Gate-tunable large-scale flexible monolayer MoS_(2)devices for photodetectors and optoelectronic synapses显示文摘Photodetectors and optoelectronic synapses are vital for construction of artificial visual perception system.However,the hardware implementations of optoelectronic-neuromorphic devices based on conventional architecture usually suffer from poor scalability,light response range,and limited functionalities.Here,large-scale flexible monolayer MoS_(2)devices integrating photodetectors and optoelectronic synapses over the entire visible spectrum in one device have been realized,which can be used in photodetection,optical communication,artificial visual perception system,and optical artificial neural network.By modulating gate voltages,we enable MoS_(2)-based devices to be photodetectors and also optoelectronic synapses.Importantly,the MoS_(2)-based optoelectronic synapses could implement many synaptic functions and neuromorphic characteristics,such as short-term memory(STM),long-term memory(LTM),paired-pulse facilitation(PPF),long-term potentiation(LTP)/long-term depression(LTD),and“learning-experience”behavior.Furthermore,an associative learning behavior(the classical conditioning Pavlov’s dog experiment)was emulated using paired stimulation of optical and voltage pulses.These results facilitate the development of MoS_(2)-based multifunctional optoelectronic devices with a simple device structure,showing great potential for photodetection,optoelectronic neuromorphic computing,human visual systems mimicking,as well as wearable and implantable electronics. | Na Li Congli He Qinqin Wang Jianshi Tang Qingtian Zhang Cheng Shen Jian Tang Heyi Huang Shuopei Wang Jiawei Li Biying Huang Zheng Wei Yutuo Guo Jiahao Yuan Wei Yang Rong Yang Dongxia Shi Guangyu Zhang | 2022 | Nano Research2022，15，6： | 1 |
| 3 | Determination of Phosphatidylcholine Content in Zophobas morio L. by High Performance Liquid Chromatography显示文摘In this study,a high performance liquid chromatographic method was established to determine the content of phosphatidylcholine in Zophobas morio L.The samples were extracted with methyl alcohol,purified,analyzed by high performance liquid chromatography,and quantified with external standard method. The results showed a good linear correlation within the concentration range of 250- 2 000 μg / ml,and the correlation coefficient was 0. 999 6. The standard recovery rate ranged from 97% to 109%,and the relative standard deviation was lower than 2. 4%. Furthermore,phosphatidylcholine contents of Z. morio individuals at different growth stages were determined. According to the results,the variation pattern of phosphatidylcholine contents of Z. morio with individual weight was consistent with the binomial curve,and the correlation coefficient was R2= 0. 865( P < 0. 01). This study laid methodological foundation for the development of Z. morio functional products. | Zhiying FAN Dongdong SHI Biying CHANG Ping ZHANG | 2015 | Agricultural Biotechnology2015，4，3： | 0 |
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