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| 1 | Allele-defined genome reveals biallelic differentiation during cassava evolution显示文摘Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species. | Wei Hu Changmian Ji Haitao shi Zhe Liang Zehong Ding Jianqiu Ye Wenjun Ou Gang Zhou Weiwei Tie Yan Yan Jinghao Yang Xiaoying Yang Yunxie Wei Zhiqiang Jin Jianghui Xie Ming Peng Wenquan Wang Anping Guo Biyu Xu Jianchun Guo Songbi Chen Liming Ma Mingcheng Wang Yang Zhou Xiaolong Li Ruoxi Li Siyi Guo Xinhui Xiao Zhongqing Wan Feifei An Jie Zhang Qingyun Leng Yin Li Ray Ming Kaimian Li | 2021 | Molecular Plant2021，14，6： | 8 |
| 2 | The Effects of Polyphenol Oxidase and Cycloheximide on the Early Stage of Browning in Phalaenopsis Explants显示文摘Explant browning is one of the major problems in the tissue culture process, and polyphenol oxidase(PPO), is the major proteases involved in plant tissue browning. We investigated the effects of polyphenol oxidase on the early stage of browning in explants of the orchid Phalaenopsis. Our results show that PPO activity was significantly higher in explants cultured for 3 d than in the 0 h control. The levels of PPO transcripts and PPO protein were significantly higher in explants cultured for 6 h compared to the 0 h control; these high expression levels were maintained over increasing cultivation time. Cycloheximide(CHX) treatment reduced PPO transcript levels, PPO protein levels, and PPO enzyme activity. High levels of PPO m RNA and PPO protein were detected in the cytoplasm and vascular bundles of Phalaenopsis explants cultured for 6 h compared to explants cultured for 0 h, 24 h, and 3 d. CHX treatment did not significantly affect the distribution of PPO m RNA and PPO protein in explant tissues, but their levels were significantly lower than those of the untreated control. | XU Chuanjun RU Zhiwei LI Ling ZENG Biyu HUANG Junmei HUANG Wen HU Ou | 2015 | Horticultural Plant Journal2015，1，3： | 2 |
| 3 | Genome-wide identification and expression analysis of the β-amylase genes strongly associated with fruit development,ripening, and abiotic stress response in two banana cultivars显示文摘β-amylase(BAM) is an important enzyme involved in conversion of starch to maltose in multiple biological processes in plants. However, there is currently insufficient information on the BAM gene family in the important fruit crop banana. This study identified 16 BAM genes in the banana genome. Phylogenetic analysis showed that Ma BAMs were classified into four subfamilies. Most Ma BAMs in each subfamily shared similar gene structures. Conserved motif analysis showed that all identified Ma BAM proteins had the typical glyco hydro14 domains. Comprehensive transcriptomic analysis of two banana genotypes revealed the expression patterns of Ma BAMs in different tissues, at various stages of fruit development and ripening, and in responses to abiotic stresses. Most Ma BAMs showed strong transcript accumulation changes during fruit development and late-stage ripening. Some Ma BAMs showed significant changes under cold, salt, and osmotic stresses. This finding indicated that Ma BAMs might be involved in regulating fruit development, ripening, and responses to abiotic stress. Analysis of five hormone-related and seven stressrelevant elements in the promoters of Ma BAMs further revealed that BAMs participated in various biological processes. This systemic analysis provides new insights into the transcriptional characteristics of the BAM genes in banana and may serve as a basis for further functional studies of such genes. | Hongxia MIAO Peiguang SUN Yulu MIAO Juhua LIU Jianbin ZHANG Caihong JIA Jingyi WANG Zhuo WANG Zhiqiang JIN Biyu XU | 2016 | Frontiers of Agricultural Science and Engineering2016，3，4： | 2 |
| 4 | Molecular identification of the key starch branching enzyme-encoding gene SBE2.3 and its interacting transcription factors in banana fruits显示文摘Starch branching enzyme(SBE)has rarely been studied in common starchy banana fruits.For the first time,we report here the molecular characterization of seven SBE(MaSBE)and six SBE(MbSBE)genes in the banana A-and B-genomes,respectively,which could be classified into three distinct subfamilies according to genome-wide identification.Systematic transcriptomic analysis revealed that six MaSBEs and six MbSBEs were expressed in the developing banana fruits of two different genotypes,BaXi Jiao(BX,AAA)and Fen Jiao(FJ,AAB),among which MaSBE2.3 and MbSBE2.3 were highly expressed.Transient silencing of MaSBE2.3 expression in banana fruit discs led to a significant decrease in its transcription,which coincides with significant reductions in total starch and amylopectin contents compared to those of empty vector controls.The suggested functional role of MaSBE2.3 in banana fruit development was corroborated by its transient overexpression in banana fruit discs,which led to significant enhancements in total starch and amylopectin contents.A number of transcription factors,including three auxin response factors(ARF2/12/24)and two MYBs(MYB3/308),that interact with the MaSBE2.3 promoter were identified by yeast one-hybrid library assays.Among these ARFs and MYBs,MaARF2/MaMYB308 and MaARF12/MaARF24/MaMYB3 were demonstrated via a luciferase reporter system to upregulate and downregulate the expression of MaSBE2.3,respectively. | Hongxia Miao Peiguang Sun Qing Liu Juhua Liu Caihong Jia Dongfang Zhao Biyu Xu Zhiqiang Jin | 2020 | Horticulture Research2020，7，1： | 2 |
| 5 | Physio-logical Changes during the Process of Pericarp Browning in the Postharvest Litchi显示文摘 | Wang Jiabao Wang Xiangshe Xu Biyu | | 0，，05： | 1 |
| 6 | Cold stress responsive microRNAs and their targets in Musa balbisiana显示文摘Cold stress is an environmental factor affecting plant development and production. Recently,micro RNAs(mi RNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. Although mi RNAs and their targets have been identified in several banana species, their participation during cold accumulation in banana remains unknown. In this study, two small RNA libraries were generated from micropropagated plantlets of Musa balbisiana grown at normal and low temperature(5°C).A total of 69 known mi RNAs and 32 putative novel mi RNAs were detected in the libraries by Solexa sequencing. Sixty-four cold-inducible mi RNAs were identified through differentially expressed mi RNAs analysis. Among 43 mi RNAs belonging to 26 conserved mi RNA families with altered expression, 18 were upregulated and 25 downregulated under cold stress. Of21 putative novel mi RNAs with altered expression, four were downregulated and 17 upregulated. Furthermore,eight mi RNAs were validated by stem-loop q RT-PCR and their dynamic differential expression was analyzed. In addition, 393 target genes of 58 identified cold-responsive mi RNAs were predicted and categorized by function.These results provide important information for further characterization and functional analysis of cold-responsive mi RNAs in banana. | Jingyi WANG Juhua LIU Caihong JIA Hongxia MIAO Jianbin ZHANG Zhuo WANG Biyu XU Zhiqiang JIN | 2016 | Frontiers of Agricultural Science and Engineering2016，3，4： | 1 |
| 7 | Study of the martensite structure at the weld interface and fracture toughness of dissimilar mental joint 显示文摘 | Wang Zhihui Xu Biyu Ciqi Y E | 1993 | Welding Research Supplement1993，，5： | 1 |
| 8 | Activation of salicylic acid metabolism and signal transduction can enhance resistance to Fusarium wilt in banana ( Musa acuminata L. AAA group, cv. Cavendish)显示文摘 | Zhuo Wang Caihong Jia Jingyang Li Suzhen Huang Biyu Xu Zhiqiang Jin | 2015 | Functional & Integrative Genomics2015，，1： | 1 |
| 9 | MaDREB1F confers cold and drought stress resistance through common regulation of hormone synthesis and protectant metabolite contents in banana显示文摘Adverse environmental factors severely affect crop productivity.Improving crop resistance to multiple stressors is an important breeding goal.Although CBFs/DREB1s extensively participate in plant resistance to abiotic stress,the common mechanism underlying CBFs/DREB1s that mediate resistance to multiple stressors remains unclear.Here,we show the common mechanism for MaDREB1F conferring cold and drought stress resistance in banana.MaDREB1F encodes a dehydration-responsive element binding protein(DREB)transcription factor with nuclear localization and transcriptional activity.MaDREB1F expression is significantly induced after cold,osmotic,and salt treatments.MaDREB1F overexpression increases banana resistance to cold and drought stress by common modulation of the protectant metabolite levels of soluble sugar and proline,activating the antioxidant system,and promoting jasmonate and ethylene syntheses.Transcriptomic analysis shows that MaDREB1F activates or alleviates the repression of jasmonate and ethylene biosynthetic genes under cold and drought conditions.Moreover,MaDREB1F directly activates the promoter activities of MaAOC4 and MaACO_(2)0 for jasmonate and ethylene syntheses,respectively,under cold and drought conditions.MaDREB1F also targets the MaERF11 promoter to activate MaACO_(2)0 expression for ethylene synthesis under drought stress.Together,our findings offer new insight into the common mechanism underlying CBF/DREB1-mediated cold and drought stress resistance,which has substantial implications for engineering cold-and drought-tolerant crops. | Yi Xu Wei Hu Shun Song Xiaoxue Ye Zehong Ding Juhua Liu Zhuo Wang Jingyang Li Xiaowan Hou Biyu Xu Zhiqiang Jin | 2023 | Horticulture Research2023，10，2： | 0 |
| 10 | Flowering-time regulation by the circadian clock:From Arabidopsis to crops显示文摘Precise timing of flowering in plants is critical for their growth and reproductive processes.One factor controlling flowering time is the cycle of light and darkness within a day,known as the photoperiod.Plants are classified into long-day,short-day,and day-neutral plants based on light requirements for floral initiation.Although the molecular mechanisms that govern this differentiation remain incompletely understood,studies have consistently shown that the circadian clock plays a central role in regulating photoperiod response across diverse plant species.However,there is a scarcity of reviews describing the regulatory network linking the circadian clock with photoperiodic flowering.This review summarizes that regulatory network,focusing on the distinct roles of clock genes in long-day and short-day plants.We also discuss the strategies of clock gene mutations contributing to geographic variation in longday and short-day crops. | Mingkang Yang Wenjie Lin Yarou Xu Biyu Xie Baiyin Yu Liang Chen Wei Huang | 2024 | The Crop Journal2024，12，1： | 0 |
| 11 | Efficient regeneration system applicable to five Musa cultivars显示文摘Banana(Musa spp.) is an important staple food, economic crop, and nutritional fruit worldwide.Hybridization is seriously hampered by the long generation time, polyploidy, and sterility of most cultivars.Establishment of an efficient regeneration and transformation system for banana is critical for their genetic improvement. An efficient and reproducible transformation system for banana using direct organogenesis was developed. Media containing benzylaminopurine(BA)combined with one of four other growth regulators was evaluated for the regeneration efficiency of five Musa cultivars and the ability to induce/support development of new banana shoots. The result indicated that the greatest number of shoots per explant for all five Musa cultivars was obtained using MS medium supplemented with8.9 mmol$L–1BA and 9.1 mmol$L–1thidiazuron(TDZ).In 240–270 d, one immature male flower could regenerate between 380 and 456, 310–372, 200–240, 130–156, and100–130 well-developed shoots for Gongjiao, Red banana, Rose banana, Baxi, and Xinglongnaijiao, respectively. Such a system will facilitate molecular breeding and functional genomics of banana. | Juhua LIU Peiguang SUN Jing ZHANG Jiashui WANG Jianbin ZHANG Jingyi WANG Caihong JIA Pengzhao GAO Biyu XU Zhiqiang JIN | 2016 | Frontiers of Agricultural Science and Engineering2016，3，4： | 0 |
| 12 | Intact regulation of G1/S transition renders esophageal squamous cell carcinoma sensitive to PI3Kαinhibitors显示文摘Phosphatidylinositol 3-kinase alpha(PI3Kα)inhibitors are currently evaluated for the therapy of esophageal squamous cell carcinoma(ESCC).It is of great importance to identify potential biomarkers to predict or monitor the efficacy of PI3Kαinhibitors in an aim to improve the clinical responsive rate in ESCC.Here,ESCC PDXs with CCND1 amplification were found to be more sensitive to CYH33,a novel PI3Kα-selective inhibitor currently in clinical trials for the treatment of advanced solid tumors including ESCC.Elevated level of cyclin D1,p21 and Rb was found in CYH33-sensitive ESCC cells compared to those in resistant cells.CYH33 significantly arrested sensitive cells but not resistant cells at G1 phase,which was associated with accumulation of p21 and suppression of Rb phosphorylation by CDK4/6 and CDK2.Hypo-phosphorylation of Rb attenuated the transcriptional activation of SKP2 by E2F1,which in turn hindered SKP2-mediated degradation of p21 and reinforced accumulation of p21.Moreover,CDK4/6 inhibitors sensitized resistant ESCC cells and PDXs to CYH33.These findings provided mechanistic rationale to evaluate PI3Kαinhibitors in ESCC patients harboring amplified CCND1 and the combined regimen with CDK4/6 inhibitors in ESCC with proficient Rb. | Xu Zhang Yuxiang Wang Xi Zhang Yanyan Shen Kang Yang Qingyang Ma Yuemei Qiao Jiajie Shi Yi Wang Lan Xu Biyu Yang Gaoxiang Ge Landian Hu Xiangyin Kong Chunhao Yang Yi Chen Jian Ding Linghua Meng | 2023 | Signal Transduction and Targeted Therapy2023，8，5： | 0 |
| 13 | Evaluating and visualizing QoS of service providers in knowledgeintensive crowdsourcing:a combined MCDM approach显示文摘Purpose-As the number of joined service providers(SPs)in knowledge-intensive crowdsourcing(KI-C)continues to rise,there is an information overload problem for KI-C platforms and consumers to identify qualified SPs to complete tasks.To this end,this paper aims to propose a quality of service(QoS)evaluation framework for SPs in KI-C to effectively and comprehensively characterize the QoS of SPs,which can aid the efficient selection of qualified SPs.Design/methodology/approach-By literature summary and discussion with the expert team,a QoS evaluation indicator system for SPs in KI-C based on the SERVQUAL model is constructed.In addition,the Decision Making Trial and Evaluation Laboratory(DEMATEL)method is used to obtain evaluation indicators’weights.The SPs are evaluated and graded by the Technique for Order Preference by Similarity to Ideal Solution(TOPSIS)and rank-sum ratio(RSR),respectively.Findings-A QoS evaluation indicator system for SPs in KI-C incorporating 13 indicators based on SERVQUAL has been constructed,and a hybrid methodology combining DEMATEL,TOPSIS and RSR is applied to quantify and visualize the QoS of SPs.Originality/value-The QoS evaluation framework for SPs in KI-C proposed in this paper can quantify and visualize the QoS of SPs,which can help the crowdsourcing platform to realize differentiated management for SPs and assist SPs to improve their shortcomings in a targeted manner.And this is the first paper to evaluate SPs in KI-C from the prospect of QoS. | Shixin Xie Xu Wang Biyu Yang Longxiao Li Jinfeng Yu | 2022 | International Journal of Intelligent Computing and Cybernetics2022，15，2： | 0 |
| 14 | Application of acidic protease in the pickling to simplify the pelt bating process显示文摘Traditionally,universally used pelt bating technologies rely on the application of trypsin,neutral and alkaline microbial proteases but suffer from complicated operation,limited bating efficiency and unsatisfac-tory leather performance.Therefore,devising a new pelt bating approach to achieve high bating efficiency and excellent leather performance has always been wished for by the leather industry.To pursue this goal,years of persistent research work enabled us to develop a novel approach for pelt bating by means of acidic proteases in pickling process.Initially,basic enzymatic characteristics and bating effectiveness of several typical acidic pro-teases in pelt pickling medium were investigated;then,the bating effectiveness through the quantitative charac-terization of protease activity of the optimal acidic protease was compared with that of the conventional bating enzyme.The results indicated that all of the selected acidic proteases had good salt-tolerance and exhibited optimum activity at pH 3.0–4.0.The novel pickling-bating method based on microbial origin acidic protease L80A led to an outstanding performance on pelt bating at the dosage of 150 U/mL of collagenolytic activity.The bating effectiveness of acidic protease L80A was comparable to and even better than that of trypsin BEM due to its mod-erate proteolytic ability.Moreover,the deep and even penetration of acidic protease in the pelt permitted it to produce soft,organoleptically stable and overall better quality crust leather than that of the conventional trypsin bating method.Additionally,pelt bating was performed along with the pickling process without extra inactiva-tion and washing operation,making the bating operation more efficient,economical,and environment friendly.Results had made us to conclude that this cutting-edge acidic proteases based pickling-bating method could be the first step/way forward to replace the decades-old traditional pelt bating technology. | Xu Zhang Mengchu Gao Sadaqat Ali Chattha Yiwen Zhu Biyu Peng Yongbin Ye | 2021 | Journal of Leather Science and Engineering2021，3，1： | 0 |
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