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| 1 | Melittin induces human gastric cancer cell apoptosis via activation of mitochondrial pathway显示文摘AIM: To investigate the apoptotic effects of melittin on SGC-7901 cells via activation of the mitochondrial signaling pathway in vitro.METHODS: SGC-7901 cells were stimulated by melittin, and its effect on proliferation and apoptosis of was investigated by methyl thiazolyl tetrazolium assay, morphologic structure with transmission electron microscopy, annexin-V/propidium iodide double-staining assay, measuring mitochondrial membrane potential(MMP) levels, and analyzing reactive oxygen species(ROS) concentrations were analyzed by flow cytometry. Cytochrome C(Cyt C), apoptosis-inducing factor(AIF), endonuclease G(Endo G), second mitochondria-derived activator of caspases(Smac)/direct IAP binding protein with low isoelectric point(Diablo), and FAS were analyzed by western blot. The expression of caspase-3 and caspase-8 was measured using activity assay kits.RESULTS: Melittin was incubated at 1.0, 2.0, 4.0, or 6.0 μg/m L for 1, 2, 4, 6, or 8 h and showed a timeand concentration-dependent inhibition of SGC-7901 cell growth. Melittin induced SGC-7901 cell apoptosis, which was confirmed by typical morphological changes. Treatment with 4 μg/m L melittin induced early apoptosis of SGC-7901 cells, and the early apoptosis rates were 39.97% ± 3.19%, 59.27% ± 3.94%, and 71.50% ± 2.87% vs 32.63% ± 2.75% for 1, 2, and 4 h vs 0 h(n = 3, P < 0.05); the ROS levels were 616.53% ± 79.78%, 974.81% ± 102.40%, and 1330.94% ± 93.09% vs 603.74% ± 71.99%(n = 3, P < 0.05); the MMP values were 2.07 ± 0.05, 1.78 ± 0.29, and 1.16 ± 0.25 vs 2.55 ± 0.42(n = 3, P < 0.05); caspase-3 activity was significantly higher compared to the control(5492.3 ± 321.1, 6562.0 ± 381.3, and 8695.7 ± 449.1 vs 2330.0 ± 121.9), but the caspase activity of the non-tumor cell line L-O2 was not different from that of the control. With the addition of the caspase-3 inhibitor(Ac-DEVD-CHO), caspase-3 activity was significantly decreased compared to the control group(1067.0 ± 132.5 U/g vs 8695.7 ± 449.1 U/g). The expression of the Cyt C, Endo G, and AIF proteins in SGC-7901 cells was significantly higher than those in the control(P < 0.05), while the expression of the Smac/Diablo protein was significantly lower than the control group after melittin exposure(P < 0.01). Ac-DEVD-CHO did not, however, have any effect on the expression of caspase-8 and FAS in the SGC-7901 cells.CONCLUSION: Melittin can induce apoptosis of human gastric cancer(GC) cells through the mitochondria pathways, and it may be a potent agent in the treatment of human GC. | Gui-Mei Kong Wen-Hua Tao Ya-Li Diao Peng-Hua Fang Ji-Jun Wang Ping Bo Feng Qian | 2016 | World Journal of Gastroenterology2016，22，11： | 15 |
| 2 | Effects of mulberry leaf flavonoid and resveratrol on methane emission and nutrient digestion in sheep显示文摘As a new type of methane control agent, natural plant extract has been widely studied in recent years,but in vivo studies are few. This study was to investigate the effects of the dietary supplementation of 2 different polyphenols on the methane(CH4) emission and digestion metabolism in sheep. Ten healthy crossbred sheep(Dorper(?)× small-tailed Han ♀; BW 60.0 ± 1.73 kg) were used in a change-over design.The sheep were fed the following 3 diets in the present study: the basal diet(CON) with no supplementation; the basal diet supplemented with 2 g mulberry leaf flavonoid(MLF) per day per sheep; the basal diet supplemented with 0.25 g resveratrol(RES) per day per sheep. Both MLF and RES reduced CH4 emission scaled to metabolic weight per kilogram of DMI and CO2 output scaled to metabolic weight, but the effect of RES was significant(P < 0.05). Both MLF and RES significantly improved apparent digestibility of DM, OM, NDF, ADF, and nitrogen, but the effect of RES was significant(P < 0.05). Both MLF and RES significantly improved ME(P < 0.05) and reduced energy losses in CH4 emission(P > 0.05). In conclusion, MLF and RES can improve the digestibility of nutrients, the utilization of nutrients and energy, and reduce CH4 emission, but they are not conducive to nitrogen retention. | Dandan Chen Xiaolin Chen Yan Tu Bo Wang Can Lou Tao Ma Qiyu Diao | 2015 | Animal Nutrition2015，，4： | 14 |
| 3 | Bearing capacity analysis of reinforced concrete L-shaped cross-section under biaxial eccentric compression显示文摘The ultimate capacity of short L-shaped reinforced concrete(RC) mernbers subjected to biaxial eccentric compression is investigated to provide a new tool for structure engineers.On the basis of nonlinear analysis of RC arbitrary cross-section,a new simplified cross-section numerical integration method is suggested.The cross-section is divided into several triangles,and each one is integrated with Gauss integration method.And L-shaped cross-sections subjected to biaxial eccentric compression are analyzed.The interaction curves of (N-M) and (My-Mz) for L-shaped sections are derived.The analytical results are well consistent with the experimental data and other analytical results. | JIAO Junting , YE Yinghua and DIAO Bo(Department of Civil Engineering, Beijing University of Aeronautics and Astronautics, Beijing 100083, China) | 2003 | Progress in Natural Science:Materials International2003，13，6： | 12 |
| 4 | Pre-clinical study of human umbilical cord mesenchymal stem cell transplantation for the treatment of traumatic brain injury: safety evaluation from immunogenic and oncogenic perspectives显示文摘Stem cell therapy is a promising strategy for the treatment of traumatic brain injury(TBI). However, animal experiments are needed to evaluate safety;in particular, to examine the immunogenicity and tumorigenicity of human umbilical cord mesenchymal stem cells(hu MSCs) before clinical application. In this study, hu MSCs were harvested from human amniotic membrane and umbilical cord vascular tissue. A rat model of TBI was established using the controlled cortical impact method. Starting from the third day after injury, the rats were injected with 10 μL of 5 × 10^(6)/m L hu MSCs by cerebral stereotaxis or with 500 μL of 1 × 10^(6)/m L hu MSCs via the tail vein for 3 successive days. hu MSC transplantation decreased the serum levels of proinflammatory cytokines in rats with TBI and increased the serum levels of anti-inflammatory cytokines, thereby exhibiting good immunoregulatory function. The transplanted hu MSCs were distributed in the liver, lung and brain injury sites. No abnormal proliferation or tumorigenesis was found in these organs up to 12 months after transplantation. The transplanted hu MSCs negligibly proliferated in vivo, and apoptosis was gradually observed at later stages. These findings suggest that hu MSC transplantation for the treatment of traumatic brain injury displays good safety. In addition, hu MSCs exhibit good immunoregulatory function, which can help prevent and reduce secondary brain injury caused by the rapid release of inflammatory factors after TBI. This study was approved by the Ethics Committee of Wuhan General Hospital of PLA(approval No. 20160054) on November 1, 2016. | Gang Wang Hua-Ling Wu Yue-Ping Liu De-Qi Yan Zi-Lin Yuan Li Chen Qian Yang Yu-Song Gao Bo Diao | 2022 | Neural Regeneration Research2022，17，2： | 6 |
| 5 | Construction of the recombinant adenovirus vector carrying antisense multidrug resistance gene显示文摘BACKGROUND: Multidrug resistance proteins serve as transporters for chemical drugs in human malignancies. The objective of this study was to construct a homologous recombinant adenovirus carrying a reversal fragment of multidrug resistance gene 1 (mdr1) gene cDNA sequence. METHODS: The fragment of the mdr1 gene from the plasmid pHaMDRI-1 carrying the whole human mdr1 cDNA sequence was inserted reversely into the shuttle plasmid pAdTrack-CMV of adenoviral vector system AdEasy. The homologous recombination process was taken place in E. coli BJ5183 with the backbone plasmid pAdEasy-1. After packaging in 293 cells, recombinant adenoviral plasmid was generated. The recombinant adenoviral plasmid was identified by polymerase chain reaction (PCR), restriction endonucleases digest, DNA sequence analysis and fluorescence microscopic photograph, respectively. RESULTS: The recombinant adenovirus pAdEasy-GFPASmdr1 was successfully constructed and identified by PCR, restriction digest, and sequencing with strong green fluorescence expression in fluorescence microscopic photograph. CONCLUSIONS: The recombinant adenoviral mdr1 vector would introduce the antisense mdr1 gene into the human multidrug resistance hepatocellular cell fine effectively, which would provide an experimental basis to study the multidrug resistance in human hepatocellular carcinoma. | Bo Li, Xing-Hua Gou, Lin Chen, De-Hua Li, Yong-Heng Zhao, Lei Han, Lan-Ying Zhao and Jian-ping Gong Department of Hepatobiliary Surgery, Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China Genetic Engineering Laboratory, Chengdu Diao Group Co. Ltd., Chengdu 610041, China and Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，1： | 6 |
| 6 | Rigid-Plastic Dynamic Response of Reinforced Concrete Bridge Pier Impacted by Automobile显示文摘Bridge piers are impacted by autos sometimes. The pier usually has not been destroyed after once impact by auto. But there are few research on damage which will affect pier's capability, and most relative studies have focused the problems on piers impacted by vessels. The methods involve mainly sutra experience theory, numerical analysis, and experimental method. Owing to the complicacy of the bridge pier impacted by a vessel, there are few research derived with the sutra mechanics model and the piers impacted by autos. The dynamic response is studied here under the assumption of the rigid-plastic small-deformation for the pier impacted by auto. According to the Parkes beam model, the rigid-plastic theoretical solution is deduced. The final deformation is calculated by a practical example for the pier impacted by auto. | ZHENG Xiaoning DIAO Bo YE Yinghua | 2006 | Transactions of Tianjin University2006，12，B09： | 4 |
| 7 | Artificial intelligence-assisted automatic and index-based microbial single-cell sorting system for One-Cell-One-Tube显示文摘Identification,sorting,and sequencing of individual cells directly from in situ samples have great potential for in-depth analysis of the structure and function of microbiomes.In this work,based on an artificial intelligence(AI)-assisted object detection model for cell phenotype screening and a cross-interface contact method for single-cell exporting,we developed an automatic and index-based system called EasySort AUTO,where individual microbial cells are sorted and then packaged in a microdroplet and automatically exported in a precisely indexed,“One-Cell-One-Tube”manner.The target cell is automatically identified based on an AI-assisted object detection model and then mobilized via an optical tweezer for sorting.Then,a crossinterface contact microfluidic printing method that we developed enables the automated transfer of cells from the chip to the tube,which leads to coupling with subsequent single-cell culture or sequencing.The efficiency of the system for single-cell printing is>93%.The throughput of the system for single-cell printing is~120 cells/h.Moreover,>80%of single cells of both yeast and Escherichia coli are culturable,suggesting the superior preservation of cell viability during sorting.Finally,AI-assisted object detection supports automated sorting of target cells with high accuracy from mixed yeast samples,which was validated by downstream single-cell proliferation assays.The automation,index maintenance,and vitality preservation of EasySort AUTO suggest its excellent application potential for single-cell sorting. | Zhidian Diao Lingyan Kan Yilong Zhao Jingyun Song Chen Wang Yang Liu Fengli Zhang Teng Xu Rongze Chen Xixian Wang Xiaoyan Jing Jian Xu Yuandong Li Bo Ma | 2022 | mLife2022，1，4： | 2 |
| 8 | Adenoviral 15-lipoxygenase-1 gene transfer inhibits hypoxia-induced proliferation of retinal microvascular endothelial cells in vitro显示文摘AIM: To investigate whether 15-Lipoxygenase-1 (15-LOX-1) plays an important role in the regulation of angiogenesis, inhibiting hypoxia-induced proliferation of retinal microvascular endothelial cells (RMVECs) and the underlying mechanism.METHODS: Primary RMVECs were isolated from the retinas of C57/BL6J mice and identified by an evaluation for FITC-marked CD31. The hypoxia models were established with the Bio-bag and evaluated with a blood-gas analyzer. Experiments were performed using RMVECs treated with and without transfer Ad-15-LOX-1 or Ad-vector both under hypoxia and normoxia condition at 12, 24, 48, 72 hours. The efficacy of the gene transfer was assessed by immunofluorescence staining. Cells proliferation was evaluated by the CCK-8 method. RNA and protein expressions of 15-LOX-1, VEGF-A, VEGFR-2, eNOs and PPAR-r were analyzed by real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blot.RESULTS: Routine evaluation for FITC-marked CD31 showed that cells were pure. The results of blood-gas analysis showed that when the cultures were exposed to hypoxia for more than 2 hours, the Po2 was 4.5 to 5.4 Kpa. We verified RMVECs could be infected with Ad-15-LOX-1 or Ad-vector via Fluorescence microscopy. CCK-8 analysis revealed that the proliferative capacities of RMVECs in hypoxic group were significantly higher at each time point than they were in normoxic group (P<0.05). In a hypoxic condition, the proliferative capacities of RMVECs in 15-LOX-1 group were significantly inhibited (P<0.05). Real-time RT-PCR analysis revealed that the expressions of VEGF-A, VEGF-R2 and eNOs mRNA increased in hypoxia group compared with normoxia group (P<0.01). However, the expressions of 15-LOX-1, PPAR-r mRNA decreased in hypoxia group compared with normoxia group (P<0.01). It also showed that in a hypoxic condition, the expressions of VEGF-A, VEGF-R2 and eNOs mRNA decreased significantly in 15-LOX-1 group compared with hypoxia group (P<0.01). However, 15-LOX-1 and PPAR-r mRNA increased significantly in 15-LOX-1 group compared with hypoxia group (P<0.01). There was no significant difference of the mRNA expressions between vector group and hypoxia group (P>0.05). Western blot analysis revealed that the expressions of relative proteins were also ranked in that order.CONCLUSION: Our results suggested that 15-LOX-1 and PPAR-r might act as a negative regulator of retinal angiogenesis. And the effect of 15-LOX-1 overexpression is an anti-angiogenic factor in hypoxia-induced retinal neovascularization (RNV). Overexpression 15-LOX-1 on RMVECs of hypoxia-induced RNV blocked signaling cascades by inhibiting hypoxia-induced increases in VEGF family. PPAR-r effect on VEGFR2 could be an additional mechanism whereby 15-LOX-1 inhibited the hypoxia-induced RNV. | Ying Yan, Yi-Qiao Xing | 2012 | International Journal of Ophthalmology(English edition)2012，5，5： | 2 |
| 9 | Association of assisted reproductive technology, germline de novo mutations and congenital heart defects in a prospective birth cohort study显示文摘Emerging evidence suggests that children conceived through assisted reproductive technology(ART)have a higher risk of congenital heart defects(CHDs)even when there is no family history.De novo mutation(DNM)is a well-known cause of sporadic congenital diseases;however,whether ART procedures increase the number of germline DNM(gDNM)has not yet been well studied.Here,we performed whole-genome sequencing of 1137 individuals from 160 families conceived through ART and 205 families conceived spontaneously.Children conceived via ART carried 4.59 more gDNMs than children conceived spontaneously,including 332 paternal and 1.26 maternal DNMs,after correcting for parental age at conception,cigarette smoking,alcohol drinking,and exercise behaviors.Paternal DNMs in offspring conceived via ART are characterized by C>T substitutions at CpG sites,which potentially affect protein-coding genes and are significantly associated with the increased risk of CHD.In addition,the accumulation of non-coding functional mutations was independently associated with CHD and 87.9% of the mutations were originated from the father.Among ART offspring,infertility of the father was associated with elevated paternal DNMs;usage of both recombinant and urinary follicle-stimulating hormone and high-dosage human chorionic gonadotropin trigger was associated with an increase of maternal DNMs.In sum,the increased gDNMs in offspring conceived by ART were primarily originated from fathers,indicating that ART itself may not be a major reason for the accumulation of gDNMs.Our findings emphasize the importance of evaluating the germline status of the fathers in families with the use of ART. | Cheng Wang Hong Lv Xiufeng Ling Hong Li Feiyang Diao Juncheng Dai Jiangbo Du Ting Chen Qi Xi Yang Zhao Kun Zhou Bo Xu Xiumei Han Xiaoyu Liu Meijuan Peng Congcong Chen Shiyao Tao Lei Huang Cong Liu Mingyang Wen Yangqian Jiang Tao Jiang Chuncheng Lu Wei Wu Di Wu Minjian Chen Yuan Lin Xuejiang Guo Ran Huo Jiayin Liu Hongxia Ma Guangfu Jin Yankai Xia Jiahao Sha Hongbing Shen Zhibin Hu | 2021 | Cell Research2021，31，8： | 2 |
| 10 | Standard liver weight model in adult deceased donors with fatty liver: A prospective cohort study显示文摘BACKGROUND Standard liver weight(SLW)is frequently used in deceased donor liver transplantation to avoid size mismatches with the recipient.However,some deceased donors(DDs)have fatty liver(FL).A few studies have reported that FL could impact liver size.To the best of our knowledge,there are no relevant SLW models for predicting liver size.AIM To demonstrate the relationship between FL and total liver weight(TLW)in detail and present a related SLW formula.METHODS We prospectively enrolled 212 adult DDs from West China Hospital of Sichuan University from June 2019 to February 2021,recorded their basic information,such as sex,age,body height(BH)and body weight(BW),and performed abdominal ultrasound(US)and pathological biopsy(PB).The chi-square test and kappa consistency score were used to assess the consistency in terms of FL diagnosed by US relative to PB.Simple linear regression analysis was used to explore the variables related to TLW.Multiple linear regression analysis was used to formulate SLW models,and the root mean standard error and interclass correlation coefficient were used to test the fitting efficiency and accuracy of the model,respectively.Furthermore,the optimal formula was compared with previous formulas.RESULTS Approximately 28.8%of DDs had FL.US had a high diagnostic ability(sensitivity and specificity were 86.2%and 92.9%,respectively;kappa value was 0.70,P<0.001)for livers with more than a 5%fatty change.Simple linear regression analysis showed that sex(R2,0.226;P<0.001),BH(R2,0.241;P<0.001),BW(R2,0.441;P<0.001),BMI(R2,0.224;P<0.001),BSA(R2,0.454;P<0.001)and FL(R2,0.130;P<0.001)significantly impacted TLW.In addition,multiple linear regression analysis showed that there was no significant difference in liver weight between the DDs with no steatosis and those with steatosis within 5%.Furthermore,in the context of hepatic steatosis,TLW increased positively(nonlinear);compared with the TLW of the non-FL group,the TLW of the groups with hepatic steatosis within 5%,between 5%and 20%and more than 20%increased by 0 g,90 g,and 340 g,respectively.A novel formula,namely,-348.6+(110.7 x Sex[0=Female,1=Male])+958.0 x BSA+(179.8 x FLUS[0=No,1=Yes]),where FL was diagnosed by US,was more convenient and accurate than any other formula for predicting SLW.CONCLUSION FL is positively correlated with TLW.The novel formula deduced using sex,BSA and FLUS is the optimal formula for predicting SLW in adult DDs. | Bo Li Pan-Yu Chen Yi-Fei Tan He Huang Min Jiang Zhen-Ru Wu Chen-Hao Jiang Dao-Feng Zheng Diao He Yu-Jun Shi Yan Luo Jia-Yin Yang | 2021 | World Journal of Gastroenterology2021，27，39： | 1 |
| 11 | The application of improved ant colony algorithm for emergency logistics vehicle routing problem显示文摘 | MA Chang-xi QI Bo DIAO Ai-xia | 2011 | International Journal of Advancements in Computing Technology2011，3，10： | 1 |
| 12 | Antitumor activity of bee pollen polysaccharides from Rosa rugosa显示文摘 | Bo Wang Qiyu Diao Zhongyu Zhang Yang Liu Qipin Gao Yifa Zhou Shanshan Li | 2013 | Molecular Medicine Reports2013，，5： | 1 |
| 13 | Effects of freeze-thaw cycles and seawater corrosion on the behavior of reinforced air-entrained concrete beams with persistent loads 显示文摘 | DIAO Bo ZHANG Jian YE Yinghua | 2013 | Journal of Cold Regions Engineering ASCE2013，27，1： | 1 |
| 14 | Effects of mixed corrosion, freeze-thaw cycles, and persistent loads on behavior of reinforced concrete beams 显示文摘 | DIAO Bo SUN Yang YE Yinghua | 2011 | Journal of Cold Regions Engineering ASCE2011，25，1： | 1 |
| 15 | Acanthopanax senticosus aqueous extract protects PC12 cells against hypoxic injury显示文摘Acanthopanax senticosus aqueous extract,at 10,5 and 2.5 μg/mL,lessened hypoxia-induced PC12 cell injury.Pretreatment with the extract upregulated Bcl-2 expression,downregulated Bax expression,elevated superoxide dismutase activity,diminished malondialdehyde content,relieved Ca2+ overloading,and suppressed apoptosis of PC12 cells.These effects were most pronounced at 10 μg/mL.These results suggest that Acanthopanax senticosus aqueous extract protects PC12 cells from hypoxia-induced injury in a dose-dependent manner. | Bo Diao Ying Liu Ying Tang Guozheng Xu | 2011 | Neural Regeneration Research2011，6，30： | 1 |
| 16 | Influence of the Anatomy of the Collecting System Upon Percutaneous Nephrolithotomy for Removal of Renal Staghorn Stones显示文摘 | Bo Xiao Xiao-Lin Diao Wei-Guo Hu Xin Zhang Song Chen Yu-Bao Liu Yu-Zhe Tang Wen-Jie Bai Jian-Xing Li | 2018 | Chinese Medical Journal2018，，14： | 1 |
| 17 | Effects of freeze-thaw cycles and seawater corrosion on the behavior of reinforced air-entrained concrete beams with persistent loads 显示文摘 | Diao Bo Zhang Jian e Yinghua Cheng Shaohong | 2013 | Journal of Cold Regions Engineering ASCE2013，27，1： | 1 |
| 18 | Effects of mixed corrosion, freeze-thaw eycles, and persistent loads on behavior of reinforced concrete beams 显示文摘 | Diao Bo Sun Yang Ye Yinghua Cheng Shaohong | 2011 | Journal of Cold Regions Engineering ASCE2011，25，1： | 1 |
| 19 | Using the Reax FF reactive force field for molecular dynamics simulations of the spontaneous combustion of lignite with the Hatcher lignite model显示文摘 | Chen Bo Diao Zhijun Lu Haiyun | 2014 | Fuel2014，116，： | 1 |
| 20 | Expression of a cytotoxic cationic antibacterial peptide in Escherichia coli using two fusion partners显示文摘 | Yu Qing Chen Shuang Quan Zhang Bao Cun Li Wen Qiu Bo Jiao Jie Zhang Zhen Yu Diao | 2007 | Protein Expression and Purification2007，，2： | 1 |
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