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| 1 | Prevalence of vacA, cagA and babA2 genes in Cuban Helicobacter pylori isolates显示文摘AIM: To investigate the prevalence of vacuolating cytotoxin (vacA), cytotoxin associated gene A (cagA) and blood adhesion binding antigen (babA2) genotypes of Helicobacter pylori (H pylori) isolates from Cuban dyspeptic patients. METHODS: DNA was extracted from H pylori-positive cultures taken from 130 dyspeptic patients. Genotyping was performed by PCR, using specifi c primers for vacA (s1, s2, m1, m2), cagA and babA2 genes. Endoscopic observations and histological examinations were used to determine patient pathologies. RESULTS: vacA alleles s1 , s2 , m1 and m2 were detected in 96 (73.8%), 34 (26.2%), 75 (57.7%) and 52 isolates (40%), respectively, while the cagA gene was detected in 95 isolates (73.2%). One hundred and seven isolates (82.3%) were babA2-positive. A signif icant correlation was observed between vacAs1m1 and cagA and between vacAs1m1 and babA2 genotypes (P < 0.001 and P < 0.05, respectively) and between babA2 genotype and cagA status (P < 0.05); but, no correlation was observed between vacAs1 and babA2 genotypes. Eighty fi ve (65.4%) and 73 (56.2%) strains were type 1 (vacAs1-cagA-positive) and 'triple- positive' (vacAs1-cagA-babA2-positive), respectively, and their presence was significantly associated with duodenal ulcer (P < 0.01 and P < 0.001, respectively). CONCLUSION: The distribution of the main virulence factors in the Cuban strains in this study resembled that of the Western-type strains, and the more virulent H pylori isolates were signifi cantly associated with duodenal ulcer, ulcer disease being the worst pathology observed in the group studied. | Lino E Torres Karelia Melián Arlenis Moreno Jordis Alonso Carlos A Sabatier Mayrín Hernández Ludisleydis Bermúdez Boris L Rodríguez | 2009 | World Journal of Gastroenterology2009，15，2： | 4 |
| 2 | Postoperative change of anti-Thomsen-Friedenreich and Tn IgG level: The follow-up study of gastrointestinal cancer patients显示文摘AIM: To study the influence of tumor removal on the serum level of IgG antibodies to tumor-associated Thomsen-Friedenreich (TF), Tn carbohydrate epitopes and xenogeneic αGal, and to elucidate on the change of the level during the follow-up as well as its association with the stage and morphology of the tumor and the values of blood parameters in gastrointestinal cancer. METHODS: Sixty patients with gastric cancer and 34 patients with colorectal cancer in stages Ⅰ-Ⅳ without distant metastases were subjected to follow- up. The level of antibodies in serum was determined by the enzyme-linked immunosorbent assay (ELISA) using synthetic polyacrylamide (PAA) glycoconjugates. Biochemical and haematological analyses were performed using automated equipment. RESULTS: In gastrointestinal cancer, the TF antibody level was found to have elevated significantly after the removal of G3 tumors as compared with the preoperative level (u = 278.5, P < 0.05). After surgery, the TF and Tn antibody level was elevated in the majority of gastric cancer patients (sign test, 20 vs 8, P < 0.05, and 21 vs 8, P < 0.05, respectively). In gastrointestinal cancer, the elevated postoperative level of TF, Tn and αGal antibodies was noted in most patients with G3 tumors (sign test, 22 vs 5, P < 0.01; 19 vs 6, P < 0.05; 24 vs 8, P < 0.01, respectively), but the elevation was not significant in patients with G1 + G2 resected tumors. The postoperative follow-up showed that the percentage of patients with G3 resected tumors of the digestive tract, who had a mean level of anti-TF IgG above the cut- off value (1.53), was significantly higher than that of patients with G1 + G2 resected tumors (χ2 = 3.89, all patients; χ2 = 5.34, patients without regional lymph node metastases; P < 0.05). The percentage of patients with a tumor in stage I, whose mean anti-TF IgG level remained above the cut-off value (1.26), was significantly higher than that of patients with the cancer in stages Ⅲ-Ⅳ (χ2 = 4.71, gastric cancer; χ2 = 4.11, gastrointestinal cancer; P < 0.05). The correlation was observed to exist between the level of anti-TF IgG and the count of lymphocytes (r = 0.517, P < 0.01), as well as between the level of anti- Tn IgG and that of serum CA 19-9 (r = 0.481, P < 0.05). No positive delayed-type hypersensitivity reaction in skin test challenges with TF-PAA in any of the fifteen patients, including those with a high level of anti-TF IgG, was observed. CONCLUSION: The surgical operation raises the level of anti-carbohydrate IgG in most patients, especially in those with the G3 tumor of the gastrointestinal tract. The follow-up demonstrates that after surgery the low preoperative level of TF antibodies may be considerably increased in patients with the carcinoma in its early stage but remains low in its terminal stages. The stage- and morphology-dependent immunosuppression affects the TF-antibody response and may be one of the reasons for unresponsiveness to the immunization with TF-antigens. | Eugeniy P Smorodin Oleg A Kurtenkov Boris L Sergeyev Kristel E Kodar Valentin I Chuzmarov Vladimir P Afanasyev | 2008 | World Journal of Gastroenterology2008，14，27： | 2 |
| 3 | Impact of Firm Heterogeneity on Direct and Spillover Effects of FDI: Micro Evidence from Ten Transition Countries显示文摘 | Jo?e P. Damijan Matija Rojec Boris Majcen Mark Knell | 2012 | Journal of Comparative Economics2012，，： | 2 |
| 4 | Immunochromatographic methods in food analysis显示文摘 | Boris B D Nadezhda A B Alexandr E U | 2013 | Trends in Analytical Chemistry2013，55，： | 1 |
| 5 | A 250GHz,50W,CW Second Harmonic Cyrotron显示文摘 | Kartikeyan M V Borie E Thumm M | 2007 | Int J Infrared Millimeter Waves2007，28，： | 1 |
| 6 | Design of a 42 GHz 200kW gyrotron operating at the second harmonic显示文摘 | Kartikeyan M V Borie E | 2004 | IEEE Trans Microwaves theory and techniques2004，52，2： | 1 |
| 7 | Biosynthesis of Vitamin B2 显示文摘 | Boris I Wolfgang E Nicholas S | 2005 | J Biol Chem2005，31，28： | 1 |
| 8 | Relationship between intraoperative cultures during hip arthroplasty,obesity,and the risk of early prosthetic joint infection:a prospective study of 428 patients显示文摘 | Font-Vizcarra L Tornero E Bori G | | 0，，09： | 1 |
| 9 | Effect of reflection on gyrotron operation显示文摘 | Borie E | 2001 | IEEETrans on Microwave Theory and Techniques2001，49，7： | 1 |
| 10 | Frequency of protein Z deficiency in patients with ischaemia stoke 显示文摘 | Vasse M Guegan Massardier E Bory TY | 2001 | Lancet2001，357，9260： | 1 |
| 11 | Case study of a Chinese dust plume reaching the French Alps 显示文摘 | Grousset F E Ginoux P Bory A | 2003 | Geophysical Ra- search2003，30，6： | 1 |
| 12 | Calculation of eigenmodes of tapered gyrotron resonators显示文摘 | BORIE E DUMBRAJS O | 1986 | Int J Electronics1986，60，2： | 1 |
| 13 | Increasederror-related negativity (ERN) in childhood anxiety disorderes:ERP and source localization 显示文摘 | Ladouceur Cecile D Dahl Ronald E Boris Birmaher | 2006 | Journal of Child Psychology and Psychiatry2006，47，10： | 1 |
| 14 | New insights into large eddy simulation 显示文摘 | Boris J P Grinstein F F Oran E S | 1992 | Fluid Dynamic Research1992，10，46： | 1 |
| 15 | Parameter studies for 150 GHz gyrotron operating in the TE031 mode显示文摘 | BoRIE E JODICKE B DUMBRAJS O | 1986 | Int J Electronics1986，61，6： | 1 |
| 16 | The future of the fullerenes显示文摘 | RICHARD E A BORIS I Y | 1998 | Solid State Comunication1998，107，11： | 1 |
| 17 | Design of a 42 GHz 200 kW gyrotron operating at the second harmonic显示文摘 | Kartikeyan M V Borie E | 2004 | IEEETrans Microwaves Theory and Techniques2004，52，2： | 1 |
| 18 | Seasonal variability in the origin of recent atmospheric mineral dust at North GRIP,Greenland显示文摘 | Bory A M Biscaye P E Svensson A | 2002 | Earth and Planetary Science Letters2002，196，： | 1 |
| 19 | Seasonal variability in the origin of recent atmospheric mineral dust at North GRIP Greenland 显示文摘 | Bory A J M Biscaye P E Svensson A | 2002 | Earth and Planetary Science Letters2002，196，： | 1 |
| 20 | Calculation of eigenmodes of tapered gyrotron resonators显示文摘 | Borie E Dumbrajs O | 1986 | Int J Electron1986，60，2： | 1 |
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