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| 1 | Bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury An in vivo magnetic resonance imaging tracking study显示文摘Non-invasive tracing in vivo can be used to observe the migration and distribution of grafted stem cells,and can provide experimental evidence for treatment.This study utilized adenovirus-carrying enhanced green fluorescent protein(AD5/F35-eGFP) and superparamagnetic iron oxide(SPIO)-labeled bone marrow mesenchymal stem cells(BMSCs).BMSCs,double-labeled by AD5/F35-eGFP and SPIO,were transplanted into rats with spinal cord injury via the subarachnoid space.MRI tracing results demonstrated that BMSCs migrated to the injured spinal cord over time(T2 hypointensity signals).This result was verified by immunofluorescence.These results indicate that MRI can be utilized to trace in vivo the SPIO-labeled BMSCs after grafting. | Yu Liu Boai Zhang Yi Song Yubin Deng Yanjie Jia Qiyong Gong | 2011 | Neural Regeneration Research2011，6，13： | 14 |
| 2 | Resveratrol improves cognition and reduces oxidative stress in rats with vascular dementia显示文摘Resveratrol possesses beneficial biological effects,which include anti-oxidant,anti-inflammatory and anti-carcinogenic properties.Recently,resveratrol has been shown to exhibit neuroprotective effects in models of Parkinson's disease,cerebral ischemia and Alzheimer's disease.However,its effects on vascular dementia remain unclear.The present study established a rat model of vascular dementia using permanent bilateral common carotid artery occlusion.At 8-12 weeks after model induction,rats were intragastrically administered 25 mg/kg resveratrol daily.Our results found that resveratrol shortened the escape latency and escape distances in the Morris water maze,and prolonged the time spent percentage and swimming distance percentage in the target quadrant during the probe test,indicating that resveratrol improved learning and memory ability in vascular dementia rats.Further experiments found that resveratrol decreased malonyldialdehyde levels,and increased superoxide dismutase activity and glutathione levels in the hippocampus and cerebral cortex of vascular dementia rats.These results confirmed that the neuroprotective effects of resveratrol on vascular dementia were associated with its anti-oxidant properties. | Xingrong Ma Zhikun Sun Yanru Liu Yanjie Jia Boai Zhang Jiewen Zhang | 2013 | Neural Regeneration Research2013，8，22： | 7 |
| 3 | Retrovirus-mediated delivery of an IL-4 receptor antagonist inhibits allergic responses in a murine model of asthma显示文摘This work reports the investigation of the effect of airway IL-4RA gene transfer by a recombinant retroviral vector on airway inflammation and airway responsiveness in asthmatic mice. The retrovirus-mediated delivery of IL-4RA to the airways of mice inhibited elevations of airway responsiveness and the development of allergic inflammation in asthmatic mice, and regulated the Th1/Th2 balance in OVA-sensitized and -challenged mouse models. This suggests that gene therapy is a therapeutic option for treating and controlling chronic airway inflammation and asthma symptoms. | WANG GuiLan1 & LU JiRong2* 1Department of Pediatrics, Zhongshan Boai Hospital, Zhongshan 528403, China 2Department of Pediatrics, First Hospital, Jilin University, Changchun 130021, China | 2010 | Science China(Life Sciences)2010，53，10： | 6 |
| 4 | Fasudil hydrochloride differentiates bone marrow mesenchymal stem cells into neurons via notch signaling显示文摘BACKGROUND: Notch signaling regulates bone marrow mesenchymal stem cell (MSC) proliferation, differentiation, and apoptosis, Notch signaling and Rho kinase signaling exhibit a crosstalk phenomenon with JAK/STAT, and both participate in the neuronal dendritic spine development. Inhibition of RhoA/Rho kinase signaling may regulate MSC differentiation into neuronal-like cells. OBJECTIVE: To investigate the effect of Notch1 signaling on the differentiation of rat MSCs into neurons induced by fasudil hydrochloride (C14H17N3O2S·HCl), a Rho kinase inhibitor, through a siRNA approach. DESIGN, TIME AND SETTING: An in vitro cytological experiment was performed in the Cell Laboratory of Henan Academy of Medical and Pharmaceutical Sciences between December 2007 and May 2009. MATERIALS: MSCs were obtained from Wistar rat femoral bone, fasudil hydrochloride was provided by Tianjin Chase Sun Pharmaceutical Co., Ltd. Rn-notch1-siRNa, negative control siRNA (Cy3 label) and Rn-MAPK1 control siRNA were provided by QIAGEN, Coloqne, German. METHODS: The cultured MSCs were divided into non-transfected, transfected group (transfected with Rn-Notch1-siRNA), positive control (transfected with Rn-MAPK-1 control siRNA), and negative control (transfected with negative control siRNA) groups. Fasudil hydrochloride was applied to induce MSCs to differentiate into neurons. MAIN OUTCOME MEASURES: The fluorescence expression by the transfected MSCs was observed under an inverted fluorescence microscope; the expression of Notch1 mRNA, Hes1 mRNA, and MAPK1 mRNA in MSCs was detected by reverse transcription polymerase chain reaction; the expression of Notch1 protein, nestin, neurofilament M, and glial fibrillary acidic protein was detected by immunocytochemistry. The viability of MSCs was detected by tetrazolium bromide assay. RESULTS: MSC fluorescence increased following a 72-hour siRNA transfection, with transfection efficiencies of up to (0.91 ± 0.04); the Notch1 mRNA and Hes1 mRNA expressed by transfected MSCs was significantly decreased (P < 0.05) compared with non-transfected cells. Fasudil hydrochloride induced MSCs to differentiate into neurons with greater efficiency in the transfected group (P < 0.05). CONCLUSION: Fasudil hydrochloride induces rat MSCs to differentiate into neurons; inhibition of Notch1 signaling and Hes1 expression may jointly promote the differentiation of MSCs into neurons. | Eryi Zhao Liudong Wang Quanqing Wen Wenjuan Guan Jingjing Lu Tao Peng Boai Zhang Yanjie Jia | 2010 | Neural Regeneration Research2010，5，11： | 5 |
| 5 | Gastrodin blocks neural stem cell differentiation into glial cells mediated by kainic acid显示文摘Kainic acid can simulate excitatory amino acids in vitro.Neural stem cells,isolated from newborn Wistar rats,were cultured in vitro and exposed to 100-4 000 μM kainic acid for 7 days to induce neuronal cell differentiation,causing the number of astrocytes to be significantly increased.Treatment with a combination of 0.5 mg/L gastrodin and kainic acid also caused the number of differentiated neurons to be significantly increased compared with treatment with kainic acid alone.Experimental findings suggest that gastrodin reduces the excitability of kainic acid and induces neural stem cell differentiation into neurons. | Guifang Sun Zhihao Yuan Boai Zhang Yanjie Jia Yangfei Ji Xingrong Ma Yu Liu Yanru Liu Quanqing Wen Yanling Zhao | 2012 | Neural Regeneration Research2012，7，12： | 4 |
| 6 | Human insulin-like growth factor 1-transfected umbilical cord blood neural stem cell transplantation improves hypoxic-ischemic brain injury显示文摘Human insulin-like growth factor 1-transfected umbilical cord blood neural stem cells were transplanted into a hypoxic-ischemic neonatal rat model via the tail vein. BrdU-positive cells at day 7 post-transplantation, as well as nestin- and neuron specific enolase-positive cells at day 14 were increased compared with those of the single neural stem cell transplantation group. In addition, the proportion of neuronal differentiation was enhanced. The genetically modified cell-transplanted rats exhibited enhanced performance in correctly crossing a Y-maze and climbing an angled slope compared with those of the single neural stem cell transplantation group. These results showed that human insulin-like growth factor 1-transfected neural stem cell transplantation promotes the recovery of the learning, memory and motor functions in hypoxic-ischemic rats. | Dengna Zhu Yanjie Jia Jun Wang Boai Zhang Guohui Niu Yazhen Fan | 2011 | Neural Regeneration Research2011，6，19： | 3 |
| 7 | Postbuckling Formulation for Symmetric Laminated Panels with Various Edge Support Conditions 显示文摘 | Chai Gin Boay Khong Poh Wah | 2001 | Mechanics of Composite Materials and Struc-tures2001，8，： | 1 |
| 8 | Mechanical propertis of Nomex material and Nomex honeycomb structure显示文摘 | Choon Chiang Foo Gin Boay Chai Leong Keey Seah | 2007 | Composite Structures2007，86，： | 1 |
| 9 | Low-velocity impact response of fibre–metal laminates – Experimental and finite element analysis显示文摘 | Shengqing Zhu Gin Boay Chai | 2012 | Composites Science and Technology2012，，15： | 1 |
| 10 | Low-velocity impact response of fibre–metal laminates – Experimental and finite element analysis显示文摘 | Shengqing Zhu Gin Boay Chai | 2012 | Composites Science and Technology2012，，15： | 1 |
| 11 | Free vibration of rectangular isotropic plates with and without a concentrated mass 显示文摘 | Boay C G | 1993 | Computers & Structures1993，48，3： | 1 |
| 12 | Mechanical properties of Nomex material and Nomex honeycomb structure显示文摘 | Choon Chiang Foo Gin Boay Chai Leung Keey Seah | 2006 | Composite Structure2006，19，3： | 1 |
| 13 | 诺梅克斯材料和诺梅克斯蜂窝结构的力学特性显示文摘介绍了对诺梅克斯纸和诺梅克斯蜂窝的线弹性力学特性的大量测试结果。诺梅克斯纸的基本力学特性被应用于有限元模型和诺梅克斯蜂窝结构分析。有限元计算结果与试验结果以及人们熟知的Gibson和Ashby公式计算结果进行对比,观测了诺梅克斯蜂窝模数的尺寸效应。 | Choon Chiang Foo Gin Boay Chai Leong Keey Seah | 2008 | 钢结构2008，23，2： | 1 |
| 14 | Effects of complete Freund's adjuvant on immunohistochemical distribution of IL-1β and IL-1R I in neurons and glia cells of dorsal root ganglion显示文摘Aim: To investigate the effects of complete Freund's adjuvant (CFA) on inflammatory hyperalgesia and morphological change of the coexsistence of interleukin-1 beta (IL-1β) and type I IL-1 receptor (IL-1RI) in neurons and glia cells of rat dorsal root ganglion (DRG). Methods: The pain-related parameters and the expression of IL-1RI and IL-1β positive neurons and glia cells of DRG in normal saline (NS) and adjuvant-induced arthritic (AA) group were examined with pain behavior assessment methods and immunohistochemical assay, respectively. Results: Five hours, 1 d, and 2 d after intra-articular injection of 50 uL CFA, tactile hyperalgesia induced by CFA was observed in the foot flexion and extension scores of the ipsilateral hindpaw of AA group. Three days after injection, the distribution of IL-1RI/IL-1β double-stained coexisted neurons and glia cells were observed in ipsilateral DRG of both groups. The number of IL-1β positive neurons, IL-1RI positive neurons, IL-1β/IL-1RI double-stained neurons, and IL-1RI positive glia cells in ipsilateral DRG of the AA group were higher than that of NS group (P<0.05 or P<0.01). Conclusion: The coexsistence of IL- 1β and IL- 1RI in neurons and nonneuronal cells suggests an as yet unknown autocrine and/or paracrine function of IL-1β in the DRG. The function was enhanced in articular arthritis induced by CFA and could play an important role in hyperalgesia under inflammatory conditions. | ManLI JingSHI Jun-ruiTANG DiCHEN BoAI JunCHEN Li-naWANG Fu-yuanCAO Ling-liLI Chuan-youLIN Xin-minGUAN | 2005 | Acta Pharmacologica Sinica2005，26，2： | 1 |
| 15 | Mechanical properities of Nomex material and Nomex honeycomb structure显示文摘 | Choon Chiang FOO Gin Boay Chai Leong Keey Seah | | 0，，80： | 1 |
| 16 | Activation of human monocytic cells by borrelia burgdorferi and treponem a pallidum is facilitated by CD14 and correlates with surface exposure of spirochetal lipoproteins 显示文摘 | Scllati TJ Boais DA Caimano M J | 1999 | J Immunol1999，163，4： | 1 |
| 17 | Controlling embedded generators using SMS显示文摘 | BOAI T PETER S | 2003 | Power Engineer2003，17，4： | 1 |
| 18 | Free vibration of laminated composite plates with a central circular hole 显示文摘 | Chai Gin Boay | 1996 | Composite Structures1996，35，4： | 1 |
| 19 | Dynamic phase-shifting photoelasticity显示文摘 | ASUNDI A TONG L BOAY C F | 2001 | Appl Opt2001，40，22： | 1 |
| 20 | Phase-shifting moire method with an atomic force microscope显示文摘 | Xie H M Boay C G Liu T Lu Y G Yu G Asundi A | 2001 | Applied Optics2001，40，34： | 1 |
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