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| 1 | Review of current diagnostic methods and advances in Helicobacter pylori diagnostics in the era of next generation sequencing显示文摘Helicobacter pylori(H.pylori)infection is highly prevalent in the human population and may lead to severe gastrointestinal pathology including gastric and duodenal ulcers,mucosa associated tissue lymphoma and gastric adenocarcinoma.In recent years,an alarming increase in antimicrobial resistance and subsequently failing empiric H.pylori eradication therapies have been noted worldwide,also in many European countries.Therefore,rapid and accurate determination of H.pylori’s antibiotic susceptibility prior to the administration of eradication regimens becomes ever more important.Traditionally,detection of H.pylori and its antimicrobial resistance is done by culture and phenotypic drug susceptibility testing that are cumbersome with a long turn-around-time.Recent advances in diagnostics provide new tools,like real-time polymerase chain reaction(PCR)and line probe assays,to diagnose H.pylori infection and antimicrobial resistance to certain antibiotics,directly from clinical specimens.Moreover,high-throughput whole genome sequencing technologies allow the rapid analysis of the pathogen’s genome,thereby allowing identification of resistance mutations and associated antibiotic resistance.In the first part of this review,we will give an overview on currently available diagnostic methods for detection of H.pylori and its drug resistance and their implementation in H.pylori management.The second part of the review focusses on the use of next generation sequencing technology in H.pylori research.To this end,we conducted a literature search for original research articles in English using the terms“Helicobacter”,“transcriptomic”,“transcriptome”,“next generation sequencing”and“whole genome sequencing”.This review is aimed to bridge the gap between current diagnostic practice(histology,rapid urease test,H.pylori culture,PCR and line probe assays)and new sequencing technologies and their potential implementation in diagnostic laboratory settings in order to complement the currently recommended H.pylori management guidelines and subsequently improve public health. | Daniel Pohl Peter M Keller Valentine Bordier Karoline Wagner | 2019 | World Journal of Gastroenterology2019，25，32： | 15 |
| 2 | Hypoglycaemia and dementia in diabetic patients显示文摘 | Bauduceau B Doucet J Bordier L | 2010 | Diabetes Metab2010，36，13： | 1 |
| 3 | Benefit of atrial pacing in sleep apnea syndrome显示文摘 | Garrigue S Bordier P Jais P | 2002 | N Engl J Med2002，346，6： | 1 |
| 4 | Processing, secretion, and immunoreactivity of C- terminally truncated dengue- 2 virus envelope protein expressed in insect cells by recombinant baculoviruses 显示文摘 | Deubel V Bordier M Megret F | 1991 | Virology1991，180，： | 1 |
| 5 | Benefit of atrial pacing in sleep apnea syndrome显示文摘 | Carrigue S Bordier P Jaǐs P | 2002 | N Engl J Med2002，346，6： | 1 |
| 6 | Phase separation of integral membrane proteins in Triton 114 solution显示文摘 | Bordier C J | 1981 | Biol Chem1981，256，4： | 1 |
| 7 | Formation of the Ce/Y2 03 interface: An in situ XPS study 显示文摘 | THROMAT N GAUTIER-SOYER M BORDIER G | 1996 | Surf Sci1996，345，3： | 1 |
| 8 | Radiative transfer model with polarization effects applied to organic matter显示文摘 | BORDIER C ANDRAUD C CHARRON E | 2007 | Physica B2007，394，： | 1 |
| 9 | Phase separation of integral membrane proteins in Triton X-114 solution显示文摘 | Bordier C | 1981 | Journal of Biological Chemistry1981，256，4： | 1 |
| 10 | Sleep apnoea in patients with heart failure,part I:diagnosis,definitions,prevalence,pathophysiology and haemodynamic consequences显示文摘 | Bordier P | | 0，，8： | 1 |
| 11 | Fast track rehabili- tation in colonic surgery 显示文摘 | Mrat S Rouquie D Bordier E | 2007 | Ann Fr Anesth Reanim2007，26，7： | 1 |
| 12 | Phase separation of integral membrane proteins in Triton X-114 solution 显示文摘 | Bordier C | 1981 | J Biol Chem1981，256，4： | 1 |
| 13 | Influence of bloodpressure level on urinary albumin excretion rate and erythropoietin production in diabetic patients 显示文摘 | Bordier L Le Berre JP Garcia C | 2006 | Arch Mal CoeurVaiss2006，99，78： | 1 |
| 14 | Epidemiology of the metabolic syndrome in 2045 French military personnel (EPIMIL study)显示文摘 | Bauduceau B Baights F Bordier L | 2005 | Diabet Metab2005，31，4： | 1 |
| 15 | Benefit of atrial pacing in sleep apnea syndrome显示文摘 | Garrigue S Bordier P Jais P | 2002 | N Engl J Med2002，346，： | 1 |
| 16 | Hypoglycemia and dementia in diabetic patients 显示文摘 | Bauduceau B Doucet J Bordier L | 2010 | Diabetes Metab2010，36，3： | 1 |
| 17 | Performance features of the new Bio-Rad HCV antigen and antibody assay: MonolisaTM HCV Ag-Ab ULTRA 显示文摘 | Lambert N Vermit L Bordier M et ol | 2004 | Vox Sanguinis2004，87，3： | 1 |
| 18 | Short-and long-term effects of nocturnal oxygen therapy on sleep apnea in chronic heart failure显示文摘 | Bordier P Orazio S Hofmann P | 2015 | Sleep Breath2015，19，1： | 1 |
| 19 | Benefit of atrial pacing in sleep apnea syndrome 显示文摘 | Garrigue S Bordier P Jais P | 2002 | N Engl J Med2002，346，6： | 1 |
| 20 | Riluzole and experimental parkinsonism:antagonism of MPTP-induced decrease in central dopamine levels in mice显示文摘 | Boireau A Dubedat P Bordier F | 1994 | Neuroreport1994，5，18： | 1 |
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