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| 1 | Specific and cross-reactive immune response against Mycobacterium tuberculosis antigens in mice immunized with proteoliposomes from Mycobacterium bovis BCG显示文摘Objective: To characterize the immunogenicity and the induction of cross-reactive responses against Mycobacterium tuberculosis(M. tuberculosis) of a proteoliposome(PL)from Mycobacterium bovis Bacillus Calmette–Guerin(BCG) with and without alum hydroxide(AL) as adjuvant(PLBCG-AL and PLBCG, respectively) in BALB/c mice.Methods: BALB/c mice were inoculated with phosphate buffer solution, BCG, PLBCG and PLBCG-AL. The humoral immunogenicity was determined by ELISA [immunoglobulin G(Ig G), Ig G1 and Ig G2a] and the cellular immunogenicity was evaluated in vivo by delayed type hypersensitivity. The humoral cross-reactive response against M. tuberculosis was determined by Western blot.Results: Sera from animals immunized with PLBCG-AL and PLBCG showed significant increase in specific total Ig G and Ig G1 antibodies and the presence of cross-reactive antibodies against M. tuberculosis antigens, which were more intense with the use of alum as adjuvant. Mice immunized with PLBCG and PLBCG-AL also showed a specific cellular response in vivo.Conclusions: The cellular and humoral immunogenicity of PLBCG and the capacity to induce cross-reactive responses against M. tuberculosis is in agreement with the protective capacity previously demonstrated by this vaccine candidate and supports the continuation of its evaluation in further stages. | Nadine Alvarez DaymíSerpa Ramlah Kadir Yanely Tirado Reinier Borrero Sonsire Fernández Rubén Cabrera Yolanda Valdes Caridad Zayas Reinaldo Acevedo Luis Izquierdo María Elena Sarmiento Mohd-Nor Norazmi JoséLuis Pérez Armando Acosta | 2017 | Asian Pacific Journal of Tropical Biomedicine2017，7，3： | 3 |
| 2 | Tomato Fusarium wilt suppressiveness. The relationship between the organic plant growth media and their microbial communities as characterised by Biolog ?显示文摘 | C. Borrero J. Ordovás M.I. Trillas M. Avilés | 2006 | Soil Biology and Biochemistry2006，，7： | 2 |
| 3 | Translational pancreatic cancer research:a comparative study on patient-derived xenograft models显示文摘AIM To assess the viability of orthotopic and heterotopic patient-derived pancreatic cancer xenografts implanted into nude mice.METHODS This study presents a prospective experimental analytical follow-up of the development of tumours in mice upon implantation of human pancreatic adenocarcinoma samples. Specimens were obtained surgically from patients with a pathological diagnosis of pancreatic adenocarcinoma. Tumour samples from pancreatic cancer patients were transplanted into nude mice in three different locations(intraperitoneal, subcutaneous and pancreatic). Histological analysis(haematoxylin-eosin and Masson's trichrome staining) and immunohistochemical assessment of apoptosis(TUNEL), proliferation(Ki-67), angiogenesis(CD31) and fibrogenesis(α-SMA) were performed. When a tumour xenograft reached the target size, it was reimplanted in a new nude mouse. Three sequential tumour xenograft generations were generated(F1, F2 and F3).RESULTS The overall tumour engraftment rate was 61.1%. The subcutaneous model was most effective in terms of tissue growth(69.9%), followed by intraperitoneal(57.6%) and pancreatic(55%) models. Tumour development was faster in the subcutaneous model(17.7 ± 2.6 wk) compared with the pancreatic(23.1 ± 2.3 wk) and intraperitoneal(25.0 ± 2.7 wk) models(P = 0.064). There was a progressive increase in the tumour engraftment rate over successive generations for all three models(F1 28.1% vs F2 71.4% vs F3 80.9%, P < 0.001). There were no significant differences in tumour xenograft differentiation and cell proliferation between human samples and the three experimental models among the sequential generations of tumour xenografts. However, a progressive decrease in fibrosis, fibrogenesis, tumour vascularisation and apoptosis was observed in the three experimental models compared with the human samples. All three pancreatic patient-derived xenograft models presented similar histological and immunohistochemical characteristics.CONCLUSION In our experience, the faster development andgreatest number of viable xenografts could make the subcutaneous model the best option for experimentation in pancreatic cancer. | Mercedes Rubio-Manzanares Dorado Luis Miguel Marín Gómez Daniel Aparicio Sánchez Sheila Pereira Arenas Juan Manuel Praena-Fernández Juan Jose Borrero Martín Francisco Farfán López Miguel ángel Gómez Bravo Jordi Muntané Relat Javier Padillo Ruiz | 2018 | World Journal of Gastroenterology2018，24，7： | 2 |
| 4 | Diagnostics in assisted human reproduction显示文摘 | Juan Manuel Montoya Alejandra Bernal Claudia Borrero | 2002 | Reproductive BioMedicine Online2002，，2： | 2 |
| 5 | Models and observations of sunspot penumbrae显示文摘The mysteries of sunspot penumbrae have been under an intense scrutiny for the past 10 years. During this time, some models have been proposed and refuted, while the surviving ones had to be modified, adapted and evolved to explain the ever-increasing array of observational constraints. In this contribution I will review two of the present models, emphasizing their contributions to this field, but also pinpointing some of their inadequacies to explain a number of recent observations at very high spatial resolution (0.32 ). To help explaining these new observations I propose some modifications to each of those models. These modifications bring those two seemingly opposite models closer together into a general picture that agrees well with recent 3D magneto-hydrodynamic simulations. | BORRERO Juan Manuel | 2009 | Science China(Physics,Mechanics & Astronomy)2009，52，11： | 2 |
| 6 | Quantitative trait loci for yield and yield components in an Oryza sativa×Oryza rufipogon BC2F2 population evaluated in an upland environment显示文摘 | P. Moncada C. P. Martínez J. Borrero M. Chatel H. Gauch Jr E. Guimaraes J. Tohme S. R. McCouch | 2001 | TAG Theoretical and Applied Genetics2001，，1： | 1 |
| 7 | Quantitative trait loci for yield and yield components in an Oryza sativa×Oryza rufipogon BC2F2 population evaluated in an upland environment显示文摘 | Moncada P Martine CP Borrero J | 2001 | Theor Appl Genet2001，102，1： | 1 |
| 8 | In vitro expansion of CD+13, CD+33 dendritic cell precursors from multipotent progeni tors is regulated by a discrete fas-mediated apoptotic schedule显示文摘 | Borrero M Tucci J | 1997 | J Leukoc Biol1997，62，4： | 1 |
| 9 | MRI findings of prepatellar Morel-Lavall6e effusions显示文摘 | Borrero CG Maxwell N Kavanagh E | 2008 | Skeletal Radiol2008，37，5： | 1 |
| 10 | Quantitative trait loci for yield and yield components in an Oryza sativa 2 Oryza rufipogon BC2F2 population evaluated in an upland environment显示文摘 | Moncada P Martinez C P Borrero J | 2001 | Theor Appl Genet2001，，102： | 1 |
| 11 | Selection of biological control agents against tomato Fusarium wilt and evaluation in greenhouse conditions of two selected agents in three growing media显示文摘 | Castafio R Borrero C M I Trillas M I | 2013 | BioControl2013，58，1： | 1 |
| 12 | Time and inventory dependent optimal maintenance policies for single machine workstations: An MDP approach 显示文摘 | BORRERO J S AKHAVAN-TABATABAEI R | 2013 | European Journal of Operational Research2013，228，3： | 1 |
| 13 | Diagnostics in assisted human reproduction显示文摘 | Montoya JM Bemal A Borrero C | 2002 | Reprod Biomed Online2002，5，2： | 1 |
| 14 | Target Recognition, Resistance, Immunity and Genome Mining of Class II Bacteriocins from Gram-positive Bacteria 显示文摘 | KJOS M BORRERO J OPSATA M | 2011 | Microbiology2011，157，： | 1 |
| 15 | Diagnostics in assistedhu- man reproduction显示文摘 | Montoya JM Bernal A Borrero C | 2002 | Reprod Biomed2002，5，2： | 1 |
| 16 | Quantitative trait loci for yield and yield components in an Oozn sativa xOryza rufipogon BC2 F2 population evaluated in an upland environment显示文摘 | Moncada P Maninez C P Borrero J | 2001 | Theor Appl Genet2001，102，： | 1 |
| 17 | Cloning and heterologous production of Hiracin JM79, a Sec-dependent bacteriocin produced by Enterococcus hirae DCH5, in lactic acid bacteria and Pichia pastoris显示文摘 | Sanchez J Borrero J Gomez-Sala B | 2008 | Appl Environ Microbiol2008，74，8： | 1 |
| 18 | Diagnosticsin assisted human reproduction显示文摘 | Montoya JM Bcmal A Borrero C | 2002 | Reprod Biomed Online2002，5，2： | 1 |
| 19 | Pregnancy with sperm aspiration from the proximal head of the epididymis:a new treatment for congenital absence of the vas deferens显示文摘 | Silber SJ Balroaceda J Borrero C | 1988 | Fertil Stenl1988，50，3： | 1 |
| 20 | Hereditary internal anal sphincter myopathy:the first Caribbean family显示文摘 | Zbar A P de la Portilla F Borrero J J | | 0，，01： | 1 |
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