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| 1 | 牛体内重组牛生长激素(rbST)的快速高灵敏度检测显示文摘重组牛生长激素(rbST)在一些国家和地区常被作为猪或牛的生长促进剂(用于乳牛可以增加牛奶产量),但在欧盟国家,有法规明确禁止生长激素的使用。重组牛生长激素本身是蛋白质,在生物基质中的浓度较低,且样品基质十分复杂,对其进行有效分析还存在一定难度,因而,长久以来其分析方法一直局限为酶联免疫检测法。 | Gaud Pinel Sandrine Roulet-Rochereau ylvain Chéreau Fabrice Monteau Bruno Le Bizec Paul Silcock Marie-Hélène Le Breton | 2010 | 食品安全导刊2010，，8： | 2 |
| 2 | Early dynamic transcriptomic changes during preoperative radiotherapy in patients with rectal cancer: A feasibility study显示文摘AIM: To develop novel biomarkers of rectal radiotherapy, we measured gene expression profiles on biopsies taken before and during preoperative radiotherapy. METHODS: Six patients presenting with a locally advanced rectal cancer (T>T2, N0/Nx, M0) eligible for preoperative radiotherapy (45 Gy in 25 fractions) were selected in a pilot study. Six tumor and 3 normal tissues biopsies were taken before and during radiotherapy,after a dose of 7.2 Gy at a median time of 1 h following irradiation (0:27-2:12). Tumor or normal tissue purity was assessed by a pathologist prior to RNA extraction. Mean RNA content was 23 μg/biopsy (14-37) before radiotherapy and 22.7 μg/biopsy (12-35) during radiotherapy. After RNA amplification, biopsies were analysed with 54K HG-U133A Plus 2.0 Affymetrix expression micro-arrays. Data were normalized according to MAS5 algorithm. A gene expression ratio was calculated as: (gene expression during radiotherapy-gene expression before radiotherapy)/gene expression before radiotherapy. Were selected genes that showed a ratio higher than ± 0.5 in all 6 patients. RESULTS: Microarray analysis showed that preoperative radiotherapy significantly up-regulated 31 genes and down-regulated 6 genes. According to the Gene Ontology project classification, these genes are involved in protein metabolism (ADAMDEC1 ; AKAP7 ; CAPN5 ; CLIC5 ; CPE ; CREB3L1 ; NEDD4L ; RAB27A), ion transport (AKAP7 ; ATP2A3 ; CCL28 ; CLIC5 ; F2RL2 ; NEDD4L ; SLC6A8), transcription (AKAP7 ; CREB3L1 ; ISX ; PAB-PC1L ; TXNIP), signal transduction (CAPN5 ; F2RL2 ; RA- B27A ; TNFRSF11A), cell adhesion (ADAMDEC1 ; PXDN ; SPON1 ; S100A2), immune response (CCL28 ; PXDN ; TNFRSF11A) and apoptosis (ITM2C ; PDCD4 ; PVT1). Up-regulation of 3 genes (CCL28 ; CLIC5 ; PDCD4) was detected by 2 different probes and up-regulation of 2 genes (RAB27A ; TXNIP) by 3 probes. CONCLUSION: Micro-arrays can efficiently assess early transcriptomic changes during preoperative radiotherapy for rectal cancer, and may help better understand tumor radioresistance. | Stephane Supiot Wilfried Gouraud Loc Campion Pascal Jezéquel Bruno Buecher Josiane Charrier Marie-Francoise Heymann Marc-Andre Mahé Emmanuel Rio Michel Chérel | 2013 | World Journal of Gastroenterology2013，19，21： | 2 |
| 3 | Phenotypic analysis of prostate-infiltrating lymphocytes reveals TH17 and Treg skewing显示文摘 | Sfanos KS Bruno TC Maris CH | 2008 | Clin Cancer Res2008，14，11： | 1 |
| 4 | Phenotypic analysis of prostate-infiltrating lymphocytes reveals Th17 and Treg skewing显示文摘 | Sfanos KS Bruno TC Maris CH | | 0，，11： | 1 |
| 5 | Magnetic resonance imaging in 300 cases of placenta aecreta: surgical correlation of new findings显示文摘 | Palacios Jaraquemada JM Bruno CH | 2005 | Acta Obstet Gyneco[ Scand2005，84，8： | 1 |
| 6 | Exoenzyme S from Pseudomonas aeruginosa induces apoptosis in T lymphocytes 显示文摘 | Bruno TF Woods DE Mody CH | 2000 | J Leukoc Biol2000，67，6： | 1 |
| 7 | Phenotypic analysis of prostate-infiltrating lymphocytes reveals TH17 and Treg skewing显示文摘 | Sfanos KS Bruno TC Maris CH | 2008 | Clin Cancer Res2008，14，11： | 1 |
| 8 | Phenotypic analysis of prostate-infiltrating lymphocytes reveals TH17 and Treg skewing 显示文摘 | Sfanos K S Bruno TC Marls CH | 2008 | Clinical Cancer Research2008，14，11： | 1 |
| 9 | Phenotypic analysis of prostate-infiltrating lymphocytes reveals TH17 and Treg skewing显示文摘 | Sfanos KS Bruno TC Maris CH | | 0，，11： | 1 |
| 10 | Lower Risk of Cancer in Patients on Metformin in Comparison With Those on Sulfonylurea Derivatives: Results from a large population-based follow-up study显示文摘 | Ruiter Rikje Visser Loes E van Herk-Sukel Myrthe P P Coebergh Jan-Willem W Haak Harm R Geelhoed-Duijvestijn Petronella H Straus Sabine M J M Herings Ron M C Stricker Bruno H CH | 2012 | Diabetes Care2012，，1： | 1 |
| 11 | Molecularmobility of amorphous pharmaceutical solids below their glass transition temperatures 显示文摘 | Bruno CH sheri IS | 1995 | Pharm Res1995，12，6： | 1 |
| 12 | Phenotypic analysis of prostatein filtrating lymphocytes reveals TH17 and Treg skewing显示文摘 | Sfanos KS Bruno TC Maris CH | 2008 | Clin Cancer Res2008，14，： | 1 |
| 13 | Phenotypic analysis of prostate-infiltrating lymphocytes reveals Th17 and Treg skewing 显示文摘 | Sfanos KS Bruno TC Marls CH | 2008 | Clin CancerRes2008，14，11： | 1 |
| 14 | Magnetic resonance ima ging in 300 cases of placenta accreta:Surgical correlation of new findings显示文摘 | Palacios Jaraquemada JM Bruno CH | | 0，，08： | 1 |
| 15 | Phenotypic analysis of prostate-infiltrating lymphocytes reveals TH17 and Tregskewing显示文摘 | Sfanos KS Bruno TC Maris CH | 2008 | Clin Cancer Res2008，14，11： | 1 |
| 16 | Phenotypic analysis of prostate-infiltrating lymphocytes reveals TH17 and Treg skewing 显示文摘 | Sfanos KS Bruno TC Maris CH | 2008 | Clin Cancer Res2008，14，11： | 1 |
| 17 | Phenotypic analysis of prostate-infiltrating lymphocytes reveals TH17 and Treg skewing 显示文摘 | Sfanos KS Bruno TC Maris CH | 2008 | Clin Cancer Res2008，14，11： | 1 |
| 18 | Phenotypic analy-sis of prostate-infiltrating lymphocytes reveals TH17 and Treg skewing显示文摘 | SFANOS KS BRUNO TC MARIS CH | 2008 | Clin Cancer Re2008，14，： | 1 |
| 19 | Phenotypic analysis of prostate-infiltrating lymphocytes reveals TH17 and Treg skewing显示文摘 | Sfanos KS Bruno TC Maris CH | 2008 | Clin Cancer Res2008，14，11： | 1 |
| 20 | Phenotypic analysis of prostate-infiltrating lymphocytes reveals Th17 and Treg skewing 显示文摘 | Sfanos KS Bruno TC Maris CH | 2008 | Clin Cancer Res2008，14，11： | 1 |
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