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| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | 在经历 transcatheter 的病人的 atrial 纤维性颤动的临床、预示的含意大动脉的阀门培植显示文摘 AIM: To study a cohort of consecutive patients under-going transcatheter aortic valve implantation (TAVI) and compare the outcomes of atrial fibrillation (AF) patients vs patients in sinus rhythm (SR). METHODS: All consecutive patients undergoing TAVI in our hospital were included. The AF group comprised patients in AF at the time of TAVI or with history of AF, and were compared with the SR group. Procedural, echocardiographic and follow-up variables were compared. Likewise, the CHA 2 DS 2-VASC stroke risk score and HAS-BLED bleeding risk score and antithrombotic treatment at discharge in AF patients were compared with that in SR patients. RESULTS: From a total of 34 patients undergoing TAVI, 17 (50%) were allocated to the AF group, of whom 15 (88%) were under chronic oral anticoagulation. Patients in the AF group were similar to those in the SR group except for a trend (P = 0.07) for a higher logistic EuroSCORE (28% vs 19%), and a higher prevalence of hypertension (82% vs 53%) and chronic renal failure (17% vs 0%). Risk of both stroke and bleeding was high in the AF group (mean CHA 2 DS 2-VASC 4.3, mean HAS-BLED 2.9). In the AF group, treatment at discharge included chronic oral anticoagulation in all except one case, and in association with an antiplatelet drug in 57% of patients. During a mean follow-up of 11 mo (maximum 32), there were only two strokes, none of them during the peri-procedural period: one in the AF group at 30 mo and one in the SR group at 3 mo. There were no statistical differences in procedural success, and clinical outcome (survival at 1 year 81% vs 74% in AF and SR groups, respectively, P = NS). CONCLUSION: Patients in AF undergoing TAVI show a trend to a higher surgical risk. However, in our cohort, patients in AF did not have a higher stroke rate compared to the SR group, and the prognosis was similar in both groups. | Pablo Salinas Raúl Moreno Luis Calvo Santiago Jiménez-Valero Guillermo Galeote Angel Sánchez-Recalde Teresa López-Fernández Sergio Garcia-Blas Diego Iglesias Luis Riera Isidro Moreno-Gómez Jose María Mesa Ignacio Plaza Rocio Ayala Rosa Gonzalez José-Luis López-Sendón | 2012 | World Journal of Cardiology2012，4，1： | 2 |
| 3 | Analysis of volatiles from Spanish honeys by solid-phase microextraction and gas chromatography-mass spectrometry 显示文摘 | P6rez RA S/mchez-Brunete C Calvo RM | 2002 | J Agr Food Chem2002，50，9： | 1 |
| 4 | Genotype-phenotype correlations in Charcot-Marie- Tooth disease type 2 caused by mitofusin 2 mutations 显示文摘 | Calvo J Funalot B Ouvrier RA | 2009 | Arch Neurol2009，66，12： | 1 |
| 5 | Characterization of the Aspergillus parasiticus deltal2-desaturase gene: a role for lipid metabolism in the Aspergillus-seed interaction显示文摘 | Wilson RA Calvo AM Chang PK | 2004 | Microbiology2004，150，： | 1 |
| 6 | Scalable Document Classification 显示文摘 | Lee JM Calvo RA | 2005 | Intelligent Data Analysis2005，9，4： | 1 |
| 7 | Relationship between secondary metabolism and fungal development显示文摘 | Calvo AM Wilson RA Bok JW | | 0，，03： | 1 |
| 8 | Analysis of volatiles from Spanish honeys by solid-phase microextraction and gas chromatography-mass spectrometry显示文摘 | PEREZ RA SANCHEZ-BRUNETE C CALVO R M | 2002 | Journal of Agricultural and Food Chemistry2002，50，9： | 1 |
| 9 | Antioxidant Activity of Indigo and its Preventive Effect against Ethanol-Induced DNA Damage in Rat Gastric Mucosa显示文摘 | Elisangela Farias-Silva Maíra Cola Tamara Calvo Victor Barbastefano Anderson Ferreira Debora De Paula Michelatto Ana Alves de Almeida Clélia Hiruma-Lima Wagner Vilegas Alba Brito | 2007 | Planta Med2007，，12： | 1 |
| 10 | SlrA/SinR/SlrR in- hibits motility gene expression upstream of a hypersensitive and hysteretic switch at the level of (rD in Bacillus subtilis显示文摘 | Cozy LM Phillips AM Calvo RA | 2012 | Mol Microbiol2012，83，6： | 1 |
| 11 | 18 F-FDG positron emission tomography staging and restaging in rectal cancer treated with preoperative chemoradiation显示文摘 | Felipe A Calvo Marta Domper Raúl Matute Raúl Martínez-Lázaro José A Arranz Manuel Desco Emilio álvarez José Luis Carreras | 2004 | International Journal of Radiation Oncology Biology Physics2004，，2： | 1 |
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