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| 1 | Construction of a recombinant attenuated Salmonella typhimurium DNA vaccine carrying Helicobacter pylorihpaA显示文摘AIM: To construct a recombinant attenuated Salmonella typhimurium DNA vaccine carrying Helicobacter pylori hpaA gene and to detect its immunogenicity.METHODS: Genomic DNA of the standard H pylori strain 17 874 was isolated as the template, hpaA gene fragment was amplified by polymerase chain reaction (PCR) and cloned into pUCmT vector. DNA sequence of the amplified hpaA gene was assayed, then cloned into the eukaryotic expression vector pIRES through enzyme digestion and ligation reactions. The recombinant plasmid was used to transform competent Escherichia coliDH5α, and the positive clones were screened by PCR and restriction enzyme digestion. Then, the recombinant pIRES-hpaA was used to transform LB5000 and the recombinant plasmid isolated from LB5000 was finally used to transform SL7207. After that, the recombinant strain was grown in vitrorepeatedly. In order to iclentify the immunogenicity of the vaccinein vitro, the recombinant pIRES-hpaA was transfected to COS-7 cells using LipofectamineTM2000, the immunogenicity of expressed HpaA protein was detected with SDS-PAGE and Western blot.RESULTS: The 750-base pair hpaA gene fragment was amplified from the genomic DNA and was consistent with the sequence of H pylori hpaA by sequence analysis. It was confirmed by PCR and restriction enzyme digestion that H pylori hpaA gene was inserted into the eukaryotic expression vector pIRES and a stable recombinant live attenuated Salmonella typhimurium DNA vaccine carrying H pylori hpaA gene was successfully constructed and the specific strip of HpaA expressed by pIRES-hpaA was detected through Western blot.CONCLUSION: The recombinant attenuated Salmonella typhimurium DNA vaccine strain expressing HpaA protein with immunogenicity can be constructed and it may be helpful for further investigating the immune action of DNA vaccine in vivo. | CanXu Zhao-ShenLi Yi-QiDu Zhen-XingTu Yan-FangGong JingJin Hong-YuWu Guo-MingXu | 2005 | World Journal of Gastroenterology2005，11，1： | 6 |
| 2 | Fabrication,properties,and applications of open-cell aluminum foams:A review显示文摘Open-cell metallic foams or porous metals have a distinctive combination of excellent structural performance and superior functional characteristics,such as their light weight,energy absorption,sound absorption,heat dissipation,and electromagnetic shielding.As a primary representative of metallic foams,aluminum foam has developed into a new engineering material with many unique applications in the fields of aerospace,automotive industry,petrochemical industry,building materials,and etc.This paper summarizes the fabrication methods,properties,and applications of open-cell aluminum foams.The current status and development trends are also introduced. | Tan Wan Yuan Liu Canxu Zhou Xiang Chen Yanxiang Li | 2021 | Journal of Materials Science & Technology2021，，3： | 5 |
| 3 | High temperature deformation behavior of near γ-phase high Nb-containing TiAl alloy显示文摘 | Jianbo Li Yong Liu Bin Liu Yan Wang Peng Cao Canxu Zhou Chunjie Xiang Yuehui He | 2014 | Intermetallics2014，，： | 1 |
| 4 | Pore structure of porous Mg-1Mn-xZn alloy fabricated by metal–gas eutectic unidirectional solidification显示文摘Lotus-type porous Mg-1 wt.% Mn-xZn(x = 0 wt.%, 1 wt.% and 2 wt.%) alloys were fabricated by metal–gas eutectic unidirectional solidification(the Gasar method). Effects of Zn addition and the fabrication process on the porosity, pore diameter and microstructure of the porous Mg alloys were investigated. Zn addition from 0 wt.% to 1 wt.% and 2 wt.% to the Mg-1 wt.% Mn alloy decreased the porosity from41.2% to 36.9% and 35.8%, respectively, with the same preparation processing. In the lotus-type porous Mg-1 wt.%Mn-1 wt.%Zn alloy, the porosities and average pore diameters changed with hydrogen pressures from 0.1 to 0.6 MPa. Conical areas that were rich in elemental Zn existed below the directional pores, and precipitates were also found in conical areas. Homogeneous directional pores existed in the lower portion of the ingot, and coarser directional pores and finer non-directional pores formed in the upper part. A theoretical model of the change in porosity with hydrogen pressure agreed well with the calculated porosities in the steady bubble growing area. The compressive strength of Mg-1 wt.Mn-Zn alloys can be increased by around 20 MPa through rising Zinc content from 1 wt.% to 2 wt.%, which basically linearly decline with the increasing of porosity. This work provides the basis for Gasar Mg-Zn-Mn alloy synthesis in biological applications and shows that the Gasar process is a promising method to fabricate Mg-Zn-Mn alloys with directional pores and a controllable pore structure. | Canxu Zhou Gangqiang Liang Yuan Liu Huawei Zhang Xiang Chen Yanxiang Li | 2022 | Journal of Magnesium and Alloys2022，10，8： | 0 |
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