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| 1 | Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells显示文摘AIM: To investigate and compare the hepatogenic transdifferentiation of adipose tissue-derived stem cells (ADSC) and bone marrow-derived mesenchymal stem cells (BMSC) in vitro. Transdifferentiation of BMSC into hepatic cells in vivo has been described. Adipose tissue represents an accessible source of ADSC, with similar characteristics to BMSC.METHODS: BMSCs were obtained from patients under-going total hip arthroplasty and ADSC from human adi-pose tissue obtained from lipectomy. Cells were grown in medium containing 15% human serum. Cultures were serum deprived for 2 d before cultivating under similar pro-hepatogenic conditions to those of liver develop-ment using a 2-step protocol with sequential addition of growth factors, cytokines and hormones. Hepatic differ-entiation was RT-PCR-assessed and liver-marker genes were immunohistochemically analysed. RESULTS: BMSC and ADSC exhibited a fibroblastic mor-phology that changed to a polygonal shape when cells differentiated. Expression of stem cell marker Thy1 de-creased in differentiated ADSC and BMSC. However, the expression of the hepatic markers, albumin and CYPs increased to a similar extent in differentiated BMSC and ADSC. Hepatic gene activation could be attributed to in-creased liver-enriched transcription factors (C/EBPβ and HNF4α), as demonstrated by adenoviral expression vec-tors.CONCLUSION: Mesenchymal stem cells can be induced to hepatogenic transdifferentiation in vitro. ADSCs have a similar hepatogenic differentiation potential to BMSC, but a longer culture period and higher proliferation ca-pacity. Therefore, adipose tissue may be an ideal source of large amounts of autologous stem cells, and may become an alternative for hepatocyte regeneration, liver cell transplantation or preclinical drug testing. | Raquel Taléns-Visconti Ana Bonora Ramiro Jover Vicente Mirabet Francisco Carbonell José Vicente Castell María José Gómez-Lechón | 2006 | World Journal of Gastroenterology2006，12，36： | 35 |
| 2 | Evolution and advances in laparoscopic ventral and incisional hernia repair显示文摘Primary ventral hernias and ventral incisional hernias have been a challenge for surgeons throughout the ages. In the current era, incisional hernias have increased in prevalence due to the very high number of laparotomies performed in the 20 th century. Even though minimally invasive surgery and hernia repair have evolved rapidly, general surgeons have yet to develop the ideal, standardized method that adequately decreases common postoperative complications, such as wound failure, hernia recurrence and pain. The evolution of laparoscopy and ventral hernia repair will be reviewed, from the rectoscopy of the 4th century to the advent of laparoscopy, from suture repair to the evolution of mesh reinforcement. The nuances of minimally invasive ventral and incisional hernia repair will be summarized, from preoperative considerations to variations in intraoperative practice. New techniques have become increasingly popular, such as primary defect closure, retrorectus mesh placement, and concomitant component separation. The advent of robotics has made some of these repairs more feasible, but only time and well-designed clinical studies will tell if this will be a durable modality for ventral and incisional hernia repair. | Alan L Vorst Christodoulos Kaoutzanis Alfredo M Carbonell Michael G Franz | 2015 | World Journal of Gastrointestinal Surgery2015，7，11： | 10 |
| 3 | Successful correction of the human beta-thalassemia major phenotype using a lentiviral vector显示文摘 | Puthenveetll G ScholesJ Carbonell D | 2004 | Blood2004，104，12： | 1 |
| 4 | Determination of polystyrene-carbon dioxide- decahydronaphthalene solution properties by high pressure dynamic light scattering显示文摘 | Dong L B Carbonell R G Roberts G W | 2009 | Polymer2009，50，24： | 1 |
| 5 | Characterization of a peptide affinity support that binds selectively to staphylococcal enterotoxin B显示文摘 | Wang G Carbonell R G | 2005 | J Chromatogr A2005，1078，12： | 1 |
| 6 | Identification of peptide ligands generated by combinatorial chemistry that bind α-lactalbumin显示文摘 | GURGEL P V CARBONELL R G SWAISGOOD H E | 2001 | Separation Science & Technology2001，36，11： | 1 |
| 7 | Comparative QTL analysis of salinity tolerance in terms of fruit yield using two Solanum populations of F7 lines显示文摘 | Villalta I Bernet G P Carbonell E A Asins M J | | 0，，： | 1 |
| 8 | Fractionation of whey proteins with a hexapeptide ligand affinity resin显示文摘 | GURGEL P V CARBONELL R G SWAISGOOD H E | 2000 | Bioseparation2000，9，6： | 1 |
| 9 | Airlift bioreactors : Analysis of local two-phase hydrodynamics 显示文摘 | Young M A Carbonell R G Ollis D F | 1991 | AIChE Journal1991，37，3： | 1 |
| 10 | Hexamer peptide affinity resins that bind the Fc region of human immunoglobulin G显示文摘 | YANG H GURGEL P V CARBONELL R G | 2005 | Journal of Peptide Research2005，66，1： | 1 |
| 11 | Lack of Genotoxicity of the Herbicide Atrazine in Cultured Human Mutation Research-genetic显示文摘 | Ribas G Surralles J Carbonell | 1998 | Toxicology and Environment Mutagenesis1998，416，12： | 1 |
| 12 | Genotoxic e- valuation of the herbicide paraquat in cultured human lym- phocytes显示文摘 | RIBAS G SURRALLES J CARBONELL E | 1998 | Teratog Careinog Mutagen1998，17，6： | 1 |
| 13 | Removal of PCDD/Fs and DL-PCBs from fish oils by volatilisation pro- cedures 显示文摘 | Carbonelle S Eppe G Hellebosch L | 2006 | Organohalogen Compounds2006，68，： | 1 |
| 14 | Recovery strategies for parsing extragrammatical language显示文摘 | CARBONELL J G HAYES P J | 1983 | American Journal of Computational Linguistics1983，9，34： | 1 |
| 15 | Learning approaches for detecting and tracking news events显示文摘 | YANG Y CARBONELL J G BROWN R D | 1999 | 14(4) :32 - 431999，14，4： | 1 |
| 16 | Source identification of soil mercury in the Spanish islands显示文摘 | Martin J A R Carbonell G Nanos N | 2013 | Archives of Environmental Contamination and Toxicology2013，64，2： | 1 |
| 17 | 显示文摘 | Chen L A Carbonell R G Serad G A | 1999 | J Chem Technol Biotechnol1999，74，8： | 1 |
| 18 | Protective effect of N-acetyl-L-cysteine on renal failure induced by inferior vena cava occlusion显示文摘 | SALOM M G RAMIREZ P CARBONELL L F | 1998 | Transplantation1998，65，10： | 1 |
| 19 | Age-and gender-related differences in the cortical anatomical network 显示文摘 | Gong G Rosa-Neto P Carbonell F | 2009 | J Neurosci2009，29，15： | 1 |
| 20 | Genotoxity of humic acid in cultured human lymphocytes and its inter action with the herbicides alachlor and maleic hydrazide显示文摘 | Ribas G Carbonell E Creus A | 1997 | Environ Molec Mut1997，29，： | 1 |
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