| 1 | Structure and genetic diversity of natural populations of Guadua weberbaueri in the southwestern Amazon,Brazil显示文摘The Brazilian state of Acre has an extensive natural reserve of bamboo,making it one of the largest in loco gene banks.The aim of this study was to characterize the structure and genetic diversity of Guadua weberbaueri Pilg.in two populations,one native(FAPB)and the other anthropized(FAPBA),using ISSR markers.The results show that the FAPB population exhibited higher values for all estimates of population diversity.However,the FAPBA population also showed high heterozygosity,corroborated by estimated gene flow(Nm=3.9)between the populations.The study of the association between Nei’s genetic distances and the geographic distances between the populations were significantly correlated(r=0.45,p=0.01),corroborated by the dendrogram revealing two distinct groups corresponding to the collection sites,without mixing classes between populations in the same group.As for the coancestry coefficient,pairs of individuals in the first distance class were positive and significant,indicating that plants that are geographically closer share common alleles with a frequency greater than by chance,which means that there is a tendency that geographically closer individuals are related.Individuals presented similar genetic structure when the geographical distance between them was up to 56 m for FAPB and up to 156 m for FAPBA.It was concluded that anthropized environments exhibit less genetic diversity than native environments,inferring risks for species conservation if appropriate and planned management techniques are not adopted. | Glória da Silva Almeida Leal Fabrício Assis Leal Hugo Teixeira Gomes Anderson Marcos de Souza Sabina Cerruto Ribeiro Jonny Everson Scherwinski-Pereira | 2021 | Journal of Forestry Research2021，32，2： | 0 |
| 2 | Comparison of the systemic action of electroacupuncture and laserpuncture in experimental injuries on the back skin of Wistar rats(Rattus novergicus)显示文摘OBJECTIVE:To evaluate the systemic action of electroacupuncture and laserpuncture in the repair of excisional cutaneous injuries on the back of adult female Wistar rats.METHODS:Ninety animals were divided into three experimental groups:C-control;E-treated with electroacupuncture[acupoints:Feishu(BL13),Geshu(BL17),Zusanli(ST36)];L-treated with laserpuncture(same acupoints)and euthanized on the 7 th,14 th and 21 st days for tissue removal and preparation for histomorphometric and biochemical(dosages,zymography and Western blotting)analysis.RESULTS:The number of fibroblasts in the E and L groups presented higher values than the C on the14 th and 21 st days.The number of granulocytes was lower than C on the 21 st day in groups E and L.The total number of newly formed vessels increased on the 21 st day and was higher in both treatments when compared to C.The birefringent collagen fibers detected on the 21 st day was lower in groups E and L than group C.The glycosaminoglycans and hydroxyproline amount was similar among groups throughout the experimental period.The active isoform of matrixmetallo proteinase(MMP)-2 and the latent isoform of MMP-9 did not show any differences among all groups and experimental periods.The amount of collagenⅠpresented higher values in the L group on the 14 th day and in the E and L groups on the 21 st day.For typeⅢcollagen,groups E and L presented values lower than C in all experimental periods.The amount of transforming growth factor-β1 in the E and L groups showed higher amounts than C on the 7 th day and lower on the 14 th and 21 st days.For vascular endothelial growth factor,E and L groups presented similar and lower values than C on the 7 th and 14 th days,respectively,and similar on the 21 st day.CONCLUSION:The therapies evaluated in this article stimulated fibroblast proliferation,neoangiogenesis and altered the reorganization of collagen fibers in the repair of excisional injuries in female adult rats. | Ivens Takechi Nakagawa Hamilton Ricardo Alonso Fabrício Campos Kuroda JoséRoberto Passarini Junior Juan Guzman Quispe Cabanillas Fernanda Aparecida Sampaio Mendonça Gláucia Maria Tech dos Santos Andrea Aparecida de Aro Edson Rosa Pimentel Maria Esméria Corezola do Amaral Marcelo Augusto Marretto Esquisatto | 2021 | Journal of Traditional Chinese Medicine2021，41，5： | 0 |
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