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| 1 | Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells显示文摘AIM: To study the therapeutic effect of three tubeguides with electrical conductivity associated to mesenchymal stem cells(MSCs) on neuro-muscular regeneration after neurotmesis.METHODS: Rats with 10-mm gap nerve injury were tested using polyvinyl alcohol(PVA), PVA-carbon nanotubes(CNTs) and MSCs, and PVA-polypyrrole(PPy). The regenerated nerves and tibialis anterior muscles were processed for stereological studies after 20 wk. The functional recovery was assessed serially for gait biomechanical analysis, by extensor postural thrust, sciatic functional index and static sciatic functionalindex(SSI), and by withdrawal reflex latency(WRL). In vitro studies included cytocompatibility, flow cytometry, reverse transcriptase polymerase chain reaction and karyotype analysis of the MSCs. Histopathology of lung, liver, kidneys, and regional lymph nodes ensured the biomaterials biocompatibility. RESULTS: SSI remained negative throughout and independently from treatment. Differences between treted groups in the severity of changes in WRL existed, showing a faster regeneration for PVA-CNTs-MSCs(P < 0.05). At toe-off, less acute ankle joint angles were seen for PVA-CNTs-MSCs group(P = 0.051) suggesting improved ankle muscles function during the push off phase of the gait cycle. In PVA-PPy and PVA-CNTs groups, there was a 25% and 42% increase of average fiber area and a 13% and 21% increase of the 'minimal Feret's diameter' respectively. Stereological analysis disclosed a significantly(P < 0.05) increased myelin thickness(M), ratio myelin thickness/axon diameter(M/d) and ratio axon diameter/fiber diameter(d/D; g-ratio) in PVA-CNT-MSCs group(P < 0.05). CONCLUSION: Results revealed that treatment with MSCs and PVA-CNTs tube-guides induced better nerve fiber regeneration. Functional and kinematics analysis revealed positive synergistic effects brought by MSCs and PVA-CNTs. The PVA-CNTs and PVA-PPy are promising scaffolds with electric conductive properties, bio- and cytocompatible that might prevent the secondary neurogenic muscular atrophy by improving the reestablishment of the neuro-muscular junction. | Jorge Ribeiro Tiago Pereira Ana Rita Caseiro Paulo Armada-da-Silva Isabel Pires Justina Prada Irina Amorim Sandra Amado Miguel Franca Carolina Goncalves Maria Ascensao Lopes Jose Domingos Santos Dina Morais Silva Stefano Geuna Ana Lúcia Luís Ana Colette Maurício | 2015 | World Journal of Stem Cells2015，7，6： | 2 |
| 2 | Comparison of three priming techniques for onion seed lots differing in initial seed quality显示文摘 | Caseiro R Bennet M A Marcos-Filho | 2004 | Seed Science and Technology2004，32，： | 1 |
| 3 | Determination of sac- charides in atmospheric aerosol using anion-exchange high-performance liquid chromatography and pulsed-am- perometric detection显示文摘 | Caseiro A Mart I L Claeys M | 2007 | Journal of Chromatography A2007，1171，12： | 1 |
| 4 | High-spud track- ing with kernelized correlation filters 显示文摘 | HENRIQUES F CASEIRO R MARTINS P | 2015 | IEEE Transactions on Pat- tern Analysis and Machine Intelligence2015，37，3： | 1 |
| 5 | Implication of sperm chromosomal abnor- malities in recurrent abortion and multiple implantation failure 显示文摘 | Caseiro Regalo Pereira | 2015 | Re- productive BioMedicine Online2015，31，4： | 1 |
| 6 | OnJacobi quasiNijenhuis algebroids and courant-Jacobi algebroid mor?phisms显示文摘 | Caseiro R De Nicola A Nunes da CostaJ M | 2010 | Journal of Geometry and Physics2010，60，68： | 1 |
| 7 | High-speed tracking with kernelized correlation filters显示文摘 | Henriques J F Caseiro R Martins P et aL | 2015 | IEEE Transactions on Pattern Analysis and Machine Intelligence2015，37，3： | 1 |
| 8 | Comparison of three priming techniques for onion seed lots differing in initial seed quality显示文摘 | Caseiro R Bennett M A Marcos-Filho J | 2004 | Seed Science and Technology2004，32，2： | 1 |
| 9 | A nonparametric Riemannian framework on tensor field with application to foreground segmentation 显示文摘 | Caseiro R Martins P Henriques J F | 2012 | Pattern Recognition2012，45，11： | 1 |
| 10 | Levoglucosan levels at background sites in Europe for assessing the impact of biomass combustion on the European aerosol background显示文摘 | PUXBAUM H CASEIRO A SANCHEZOCHOA A | 2007 | Journal of Geophysical Research2007，112，： | 1 |
| 11 | High-speed trackingwith kernelized correlation filters显示文摘 | Henriques J F Caseiro R Martins P | 2015 | IEEE Transactions on PatternAnalysis and Machine Intelligence2015，37，3： | 1 |
| 12 | Road traffic impact on urban atmospheric aerosol loading at Oporto, Portugal 显示文摘 | Oliveira C Pio C Caseiro A | 2010 | Atmospheric Environment2010，44，： | 1 |
| 13 | Salivary proteome and peptidome profiling in type I diabetes mellitus using a quantitative approach 显示文摘 | Caseiro A Ferreira R Padraao A | 2013 | J Proteome Res2013，12，4： | 1 |
| 14 | Evaluation of methods for drying primed onion seeds显示文摘 | Caseiro R F Marcos Filho J | 2005 | Horticultura Brasileira2005，23，4： | 1 |
| 15 | Road Traffic Impact on Urban Atmospheric Aerosol Loading at Oporto, Portugal显示文摘 | Cesar Oliveira Casimiro Pio Alexandre Caseiro | 2010 | Atmospheric Environment2010，44，26： | 1 |
| 16 | High-Speed Tracking with Kernelized Correlation Filters显示文摘 | Henriques J F Caseiro R Martins P | 2015 | IEEE Transactions on Pattern Analysis&Machine Intelligence2015，37，3： | 1 |
| 17 | Prevalence of lysosomal storage diseases in Portugal 显示文摘 | PINTO R CASEIRO C LEMOS M | 2004 | Eur J Hum Genet2004，12，2： | 1 |
| 18 | High-speed tracking with kernelized correlation filters显示文摘 | Henriques J F Caseiro R Martins P | 2014 | IEEE Transactions on Pat- tern Analysis and Machine Intelligence2014，37，3： | 1 |
| 19 | Determination of saccharides in atmospheric aerosol using anion-exchange high- performance liquid chromatography and pu]sed-amperometric detection显示文摘 | CASEIRO A MARR I L CLAEYS M | 2007 | Journal of Chromatography A2007，1171，12： | 1 |
| 20 | Experi- mental evidence for a significant contribution of cellulose to indoor aerosol mass concentration显示文摘 | Cerqueira M Marques D Caseiro A Pio C | 2010 | Atmospheric Environment2010，44，： | 1 |
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