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		题名	作者	年代	出处	被引量
	1	A Stable Upstream Stem-loop Structure Enhances Selection of the First 5'-ORF-AUG as a Main Start Codon for Translation Initiation of Human ACAT1 mRNA显示文摘Human ACAT1 cDNA K1 was first cloned and functionally expressed in 1993. There are two adjacent in-frame AUG codons, AUG1397–1399 and AUG1415–1417, at 5’-terminus of the open reading frame (ORF, nt 1397–3049) of human ACAT1 mRNA corresponding to cDNA K1. In current work, these two adjacent in- frame AUGs at 5’-terminus of the predicted ORF (5’-ORF-AUGs) as start codons for translation initiation of human ACAT1 mRNA were characterized in detail. Codon mutations indicated that both of these two adja- cent 5’-ORF-AUGs can be selected as start codons but the first 5’-ORF-AUG1397–1399 is a main start codon consistent with that of the predicted ORF of human ACAT1 mRNA. Further deletion and mutation analyses demonstrated that a stable upstream stem-loop structure enhanced the selection of the first 5’-ORF-AUG1397– as a main start codon, in addition to upstream nucleotide A in the –3 position, which is a key site of Kozak 1399 sequence. In addition, result of ACAT1 enzymatic activity assay showed no obvious difference between these two ACAT1 proteins respectively initiated from the two adjacent 5’-ORF-AUGs. This work showed that a stable upstream stem-loop structure could modulate the start codon selection during translation initiation of mRNAs that contain adjacent multi-5’-ORF-AUGs.	LiYANG JiangCHEN CatherineC.Y.CHANG Xin-YingYANG Zhen-ZhenWANG Ta-YuanCHANG Bo-LiangLI	2004	Acta Biochimica et Biophysica Sinica2004，36，4：	7

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