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| 1 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 2 | Involvement of vacuolar H^+-adenosine triphoshatase activity in multidrug resistance HL60 cells显示文摘 | Marquardt D Center M S | 1991 | J Natl Cancer Inst1991，83，15： | 1 |
| 3 | Sexually transmitted diseases treatment guidelines,2006显示文摘 | Centers for Disease Control and Prevention Workowski K A Berman S M | 2006 | MMWR Recomm Rep2006，55，11： | 1 |
| 4 | The MRP gene associated with a non-p-glycoprotein multidrug resistance encodes a 190 kD membrane bound glycoprotein 显示文摘 | Krishnamachary N Center M S | 1993 | Cancer Res1993，53，16： | 1 |
| 5 | Prognostic factors in retroperitoneal sarcoma: a multivariate analysis of a series of 165 patients of the french cancer center federation sarcoma group 显示文摘 | Stoeckle E Coindre J M Bonvalot S French federation of cancer centers sarcoma group | 2001 | Cancer2001，92，： | 1 |
| 6 | Japanese encephalitis vaccines : recommendations of the Advisory Committee on Immunization Practices (ACIP) 显示文摘 | Fischer M Lindsey N Staples JE Hills S Centers for Disease C Prevention | 2010 | MM- WR Recomm Rep2010，59，1： | 1 |
| 7 | Sexually transmitted diseases treatment guidelines, 2006显示文摘 | Centers for Disease Control and Prevention WORKOWSKI K A BERMAN S M | 2006 | MMWR Reeomm Rep2006，55，11： | 1 |
| 8 | The MRP gene associated with a non-p-glycoprotein muhidrug resistance encodes a 190 kD membrane bound glycoprotein 显示文摘 | KRISHNAMACHARY N CENTER M S | 1993 | Cancer Res1993，53，16： | 1 |
| 9 | Sexually transmitted diseases treatment guidelines,2006显示文摘 | Centers for Disease Control and Prevention WORKOWSKI K A BERMAN S M | 2006 | MMWR Recomm Rep2006，55，11： | 1 |
| 10 | Sexually transmitted disease treatment guidelines 2006显示文摘 | Centers for Disease Control and Prevention Workowski K A Berman S M | 2006 | MM-WR Recomm Rep2006，4，55： | 1 |
| 11 | Tin whisker electrical short circuit characteristics: Part I显示文摘 | COUREY K ASFOUR S BAYLISS J LUDWIG L ZAPATA M CENTER L | 2008 | IEEE Transactions on Electronics Packaging Manufacturing2008，31，1： | 1 |
| 12 | Fishery, population dynamics and stock structure of frigate tuna Auxis thazard (Lacepede, 1800)exploited from Indian waters显示文摘 | Intentional Center for Living Kuwait Institute for Scientific GHOSH S SIVADAS M ABDUSSAMAD E M | 2012 | Indian Journal of Fisheries2012，59，2： | 1 |
| 13 | 产黄青霉几丁质合成酶基因的克隆及系统学分析(英文) | Namgung J Park B C Park Y D 1 Lee M S 1 Lee D H 2 Bae K S 1 Park H M (Department of Microbiology, Chungnam National University, Taejon 305 764 1 Genetic Resources Center, Korea Research Institute for Bioscience & Biotechnology, Taej | 1999 | 安徽农业大学学报1999，26，3： | 0 |
| 14 | Study of BMPR IA downstream genes related to ventricular septum defect显示文摘Cardiac-specific deletion of the receptor IA of bone morphogenetic protein, ALK3, by Cre recombinase driven under the a-MHC promoter is lethal in mid-gestation with defects in the interventricular septum. Analysis of expression of the ALK3 downstream genes is important to identify the signaling pathway for interventricular septum development. The model mice with ALK3 gene knockout via a-MHC-Cre/lox P system were bred. The mRNA expression level of control group was compared with that of test group. ALK3 downstream genes were screened using PCR-select cDNA subtraction and microarray. It was found that the mice with ALK3 gene knockout produced heart defects involving the interventricular septum. The expression of some genes such as platelet-activating factor acetylhydrolase and the transcription factor Pax-8 etc was downregulated in the test group. The box protein Pax-8 gene expression was downregulated by 7.1 times in the test group and expressed specifically in the embryonic heart (11.5 days). Furthermore, the expression of the Protein Tyrosine Kinase of Focal Adhesion Kinase subfamily (PTK) and beta subtype protein 14-3-3 was upregulated in the a-MHC-Cre+/-ALK3 F/-mice. PTK gene expression was up-regulated by 3.7 times in the test group. These data provided support that ALK3 gene played an important role during heart development. The platelet-activating factor acetylhydrolase and Pax-8 genes could be important ALK3 downstream genes in the BMP signaling pathway during interventncular septum development. PTK and beta subtype protein 14-3-3 might be regulatory factors in this pathway. | YANG DY, ZHANG JY, CHEN CX, XIE M, SPERLING S, FANG F, CHEN BX, LI XW, ZHANG HQ. Department of Cardiology, The First Affiliated Hospital, Institute for Cardiovascular Biology & Gene, Wenzhou Medical College, Wenzhou 325000, P. R.China Center for Cardiovascular Development, Baylor College of Medicine, Houston, TX 77030 Cardiovascular Genetics, Department Prof. H. Lehrach, Max-Planck-Institute for Molecular Genetics, Ihnestr. 73, 14195 Berlin, Germany | 2007 | 上海医学2007，30，S1： | 0 |
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