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| 1 | Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars显示文摘D-Psicose 3-epimerase(DPEase)is demonstrated to be useful in the bioproduction of D-psicose,a rare hexose sugar,from D-fructose,found plenty in nature.Clostridium cellulolyticum H10 has recently been identified as a DPEase that can epimerize D-fructose to yield D-psicose with a much higher conversion rate when compared with the conventionally used DTEase.In this study,the crystal structure of the C.cellulolyticum DPEase was determined.The enzyme assembles into a tetramer and each subunit shows a(β/α)8 TIM barrel fold with a Mn2+metal ion in the active site.Additional crystal structures of the enzyme in complex with substrates/products(D-psicose,D-fructose,D-tagatose and D-sorbose)were also determined.From the complex structures of C.cellulolyticum DPEase with D-psicose and D-fructose,the enzyme has much more interactions with D-psicose than D-fructose by forming more hydrogen bonds between the substrate and the active site residues.Accordingly,based on these ketohexosebound complex structures,a C3-O3 proton-exchange mechanism for the conversion between D-psicose and D-fructose is proposed here.These results provide a clear idea for the deprotonation/protonation roles of E150 and E244 in catalysis. | Hsiu-Chien Chan Yueming Zhu Yumei Hu Tzu-Ping Ko Chun-Hsiang Huang Feifei Ren Chun-Chi Chen Yanhe Ma Rey-Ting Guo Yuanxia Sun | 2012 | Protein & Cell2012，3，2： | 6 |
| 2 | Meteorin-β/Meteorin like/IL-41 attenuates airway inflammation in house dust mite-induced allergic asthma显示文摘We sought to examine the regulatory effect of Meteorin-β(Metrnβ)/Meteorin like(Metrnl)/IL-41 on lung inflammation in allergic asthma.We found that Metrnβwas elevated significantly in asthmatic patients and in mice with allergic asthma induced by house dust mite(HDM)extract.Upon exposure to HDM,Metrnβwas secreted predominantly by airway epithelial cells and inflammatory cells,including macrophages and eosinophils.The increased Metrnβeffectively blocked the development of airway hyperreactivity(AHR)and decreased inflammatory cell airway infiltration and type 2 cytokine production,which was associated with downregulated DC-mediated adaptive immune responses.Moreover,Metrnβimpaired the maturation and function of bone marrow-derived dendritic cells in vitro.Asthmatic mice adoptively transferred with dendritic cells isolated from Metrnβ-treated allergic mice displayed decreased AHR,airway inflammation,and lung injury.Metrnβalso displayed anti-inflammatory properties in immunodeficient SCID mice with allergic asthma and in in vitro 3D ALI airway models.Moreover,blockade of Metrnβby anti-Metrnβantibody treatment promoted the development of allergic asthma.These results revealed the unappreciated protective roles of Metrnβin alleviating DC-mediated Th2 inflammation in allergic asthma,providing the novel treatment strategy of therapeutic targeting of Metrnβin allergic asthma. | Xun Gao Ting-Fan Leung Gary Wing-Kin Wong Wing-Hung Ko Mengyun Cai Ellie Jiayi He Ida Miu-Ting Chu Miranda Sin-Man Tsang Ben Chung-Lap Chan Jiawei Ling Xiao Fan Liwei Lu Christopher Wai-Kei Lam Chun-Kwok Wong | 2022 | Cellular & Molecular Immunology2022，19，2： | 5 |
| 3 | Survival outcome of lobar or segmental transcatheter arterial embolization with ethanol-lipiodol mixture in treating hepatocellular carcinoma显示文摘AIM: To evaluate the clinical outcome and cost-effectiveness of transcatheter arterial ethanol-lipiodol embolotherapy on hepatocellular carcinoma (HCC).METHODS: One hundred patients with HCC who were treated only by lobar or segmental transarterial embolization (TAE) with ethanol-lipiodol mixture wereenrolled in this study. The 1st- and 2nd-year survival rates were analyzed to evaluate the feasibility of its method. These outcomes of our patients were individually correlated to the Child-Pugh classification and the computed tomographic features of HCC.RESULTS: The overall 1st- and 2nd-year survival rates were 72% and 46%, respectively. The patients were classified into three groups according to their liver function status: 68 patients as Child-Pugh class A, 26 as Child B, and 6 as Child C. Child A had better survival rate than the Child B and/or C. The 1st-year survival rates of patients with Child A-C were 84%, 50%, and 33.3% respectively and the 2nd-year survival rates were 55.5%, 28.5%, and 33.3%, respectively. According to the computed tomographic features, solitary HCC with maximum diameter less than 5 cm had the best outcome with the 1st-year survival rate of 100% and the 2nd-year survival rate of 71.4%, while solitary HCC with maximum diameter over 5 cm and multiple HCC had the 1st-year survival rates of 75% and 63.7%, respectively, and the 2nd-year survival rates of 33.3% and 44.4%, respectively. Only one patient was complicated with abscess formation and was cured with antibiotic therapy. No mortality resulted from the procedures performed.CONCLUSION: TAE with ethanol-lipiodol mixture is an economic, safe and feasible method for treating HCC, especially for the patients with smaller solitary HCC or with liver function status of Child-Pugh class A. | Yun-Chung Cheung Sheung-Fat Ko Shu-Hang Ng Siu-Cheung Chan | 2005 | World Journal of Gastroenterology2005，11，18： | 4 |
| 4 | A novel device for endoscopic submucosal dissection,the Fork knife显示文摘瞄准:用一台新奇设备介绍并且评估功效和内视镜的粘膜下层解剖(ESD ) 的技术方面,叉刀。方法:从 2004 年 3 月到 2008 年 4 月, ESD 在一个单个第三级的工作分派中心用 Flexknife (组 B ) 在使用一把叉刀(Endo FSregister )( 组 A ) 的 265 胃的损害上并且在 72 胃的损害上被执行。我们回顾地比较了 resected 标本的肿瘤,病理检查所见,和尺寸的内视镜的特征。我们也比较了在二个组之间的整块切除术率,完全的切除术率,复杂并发症,和过程时间。结果:resected 标本的吝啬的尺寸是 4.27 +/- 在组 A 和 4.29 +/- 的 1.26 厘米在组 B 的 1.48 厘米。整块切除术率是 95.8%(254/265 损害) 在组 A 并且 93.1%(67/72 ) 在组 B。没有 resected 边缘的肿瘤房间侵略的完全的 ESD 在 81.1% 被获得(215/265 ) 组 A 并且在 73.6%(53/72 ) 组 B。穿孔率是 0.8%(2/265 ) 在组 A 并且 1.4%(1/72 ) 在组 B。吝啬的过程时间是 59.63 +/- 在组 A 和 76.65 +/- 的 56.12 min 在组 B 的 70.75 min (P <
0.05 ) 。结论:叉刀(Endo FSregister ) 为临床的实践是有用的并且有的优点减少过程时间。 | Hyun Gun Kim Joo Young Cho Gene Hyun Bok Won Young Cho Wan Jung Kim Bong Min Ko Jin Oh Kim Joon Seong Lee Moon Sung Lee Chan Sup Shim | 2008 | World Journal of Gastroenterology2008，14，43： | 3 |
| 5 | Diabetic factors associated with gastrointestinal symptoms in patients with type 2 diabetes显示文摘AIM: To determine whether gastrointestinal (GI) symptoms are more frequent in type 2 diabetic patients and to examine which diabetic factors are associated with the symptoms. METHODS: Consecutive subjects with diabetes and age-/gender-matched normal controls were recruited for this study. GI symptoms were assessed using a structured questionnaire divided into two GI symptomcategories (upper and lower GI symptoms), and consisting of 11 individual symptoms. In the diabetic patient group, diabetic complications including peripheral neuropathy, nephropathy and retinopathy, glycosylated hemoglobin (HbA1c) level and diabetes duration were evaluated. RESULTS: Among the total 190 diabetic patients and 190 controls enrolled, 137 (72%) of the diabetic patients and 116 (62%) of the controls had GI symptoms. In the diabetic patient group, 83 (43%) had upper GI symptoms and 110 (58%) lower GI symptoms; in the control group, 59 (31%) had upper GI symptoms and 104 (55%) lower GI symptoms. This difference between the two groups was significant for only the upper GI symptoms (P = 0.02). Among the diabetic factors, the HbA1c level was the only independent risk factor for upper GI symptoms in the multiple logistic regression analysis (odds ratio = 2.01, 95% confidence interval: 1.02-3.95). CONCLUSION: Type 2 diabetes was associated with an increased prevalence of upper GI symptoms and these symptoms appeared to be independently linked to poor glycemic control, as measured by the HbA1c levels. | Jeong Hwan Kim Hyung Seok Park Soon Young Ko Sung Noh Hong In-Kyung Sung Chan Sub Shim Kee-Ho Song Dong-Lim Kim Sook Kyung Kim Jeeyoung Oh | 2010 | World Journal of Gastroenterology2010，16，14： | 3 |
| 6 | A MEMS ultrasound stimulation system for modulation of neural circuits with high spatial resolution in vitro显示文摘Neuromodulation by ultrasound has recently received attention due to its noninvasive stimulation capability for treating brain diseases.Although there have been several studies related to ultrasonic neuromodulation,these studies have suffered from poor spatial resolution of the ultrasound and low repeatability with a fixed condition caused by conventional and commercialized ultrasound transducers.In addition,the underlying physics and mechanisms of ultrasonic neuromodulation are still unknown.To determine these mechanisms and accurately modulate neural circuits,researchers must have a precisely controllable ultrasound transducer to conduct experiments at the cellular level.Herein,we introduce a new MEMS ultrasound stimulation system for modulating neurons or brain slices with high spatial resolution.The piezoelectric micromachined ultrasonic transducers(pMUTs)with small membranes(submm membranes)generate enough power to stimulate neurons and enable precise modulation of neural circuits.We designed the ultrasound transducer as an array structure to enable localized modulation in the target region.In addition,we integrated a cell culture chamber with the system to make it compatible with conventional cell-based experiments,such as in vitro cell cultures and brain slices.In this work,we successfully demonstrated the functionality of the system by showing that the number of responding cells is proportional to the acoustic intensity of the applied ultrasound.We also demonstrated localized stimulation capability with high spatial resolution by conducting experiments in which cocultured cells responded only around a working transducer. | Jungpyo Lee Kyungmin Ko Hyogeun Shin Soo-Jin Oh CJustin Lee Namsun Chou Nakwon Choi Min Tack Oh Byung Chul Lee Seong Chan Jun Il-Joo Cho | 2019 | Microsystems & Nanoengineering2019，5，1： | 3 |
| 7 | A critical role of IL-17 in modulating the B-cell response during H5N1 influenza virus infection显示文摘Interleukin-17(IL-17),a member of the IL-17 cytokine family,plays a crucial role in mediating the immune response against extracellular bacteria and fungi in the lung.Although there is increasing evidence that IL-17 is involved in protective immunity against H1 and H3 influenza virus infections,little is known about the role of IL-17 in the highly pathogenic H5N1 influenza virus infection.In this study,we show that H5N1-infected IL-17 knockout(KO)mice exhibit markedly increased weight loss,more pronounced lung immunopathology and significantly reduced survival rates as compared with infected wild-type controls.Moreover,the frequency of B cells in the lung were substantially decreased in IL-17 KO mice after virus infection,which correlated with reduced CXCR5 expression in B cells and decreased CXCL13 production in the lung tissue of IL-17 KO mice.Consistent with this observation,B cells from IL-17 KO mice exhibited a significant reduction in chemokine-mediated migration in culture.Taken together,these findings demonstrate a critical role for IL-17 in mediating the recruitment of B cells to the site of pulmonary influenza virus infection in mice. | Xiaohui Wang Chris CS Chan Min Yang Jun Deng Vincent KM Poon Virtual HC Leung King-Hung Ko Jie Zhou Kwok Yung Yuen Bo-Jian Zheng Liwei Lu | 2011 | Cellular & Molecular Immunology2011，8，6： | 2 |
| 8 | Intraductal balloon-guided direct peroral cholangioscopy with an ultraslim upper endoscope (with videos)显示文摘 | Jong Ho Moon Bong Min Ko Hyun Jong Choi Su Jin Hong Young Koog Cheon Young Deok Cho Joon Seong Lee Moon Sung Lee Chan Sup Shim | 2009 | Gastrointestinal Endoscopy2009，，2： | 2 |
| 9 | Overtube-balloon–assisted direct peroral cholangioscopy by using an ultra-slim upper endoscope (with videos)显示文摘 | Hyun Jong Choi Jong Ho Moon Bong Min Ko Su Jin Hong Hyun Cheol Koo Young Koog Cheon Young Deok Cho Joon Seong Lee Moon Sung Lee Chan Sup Shim | 2009 | Gastrointestinal Endoscopy2009，，4： | 2 |
| 10 | 慢性阻塞性肺疾病急性加重患者感染性致病因素分析显示文摘背景和目的:反复急性发作是慢性阻塞性肺疾病(COPD)的临床特点,同时也是患者住院的主要原因,呼吸道感染常常是急性发作的诱因。适当的经验性应用抗生素对于降低此类患者的住院率和病死率非常有意义。不同居住地的COPD患者引起急性发作的病原谱是不同的,因而地区性资料对于指导临床治疗很重要。本研究的目的是阐明我国香港地区COPD急性发作的病原学及其治疗。方法:研究人员分析了我国香港2004年5月至2005年4月因COPD急性发作而住院的患者, | Ko FW Ip M Chan PK 李翠联 曹彬 | 2007 | 中华结核和呼吸杂志2007，30，5： | 2 |
| 11 | Impact of patient education with cartoon visual aids on the quality of bowel preparation for colonoscopy显示文摘 | Jae Woong Tae Jong Chan Lee Su Jin Hong Jae Pil Han Yun Hee Lee Jong Ho Chung Hyung Geun Yoon Bong Min Ko Joo Young Cho Joon Seong Lee Moon Sung Lee | 2012 | Gastrointestinal Endoscopy2012，，4： | 2 |
| 12 | Oleanolic acid attenuates MK-801-induced schizophrenia-like behaviors in mice显示文摘 | Se Jin Park Younghwa Lee Hee Kyong Oh Hyung Eun Lee Younghwan Lee Sang Yoon Ko Boseong Kim Jae Hoon Cheong Chan Young Shin Jong Hoon Ryu | 2014 | Neuropharmacology2014，，： | 2 |
| 13 | Phenylketonuria in Hong Kong Chinese: a call for hyperphenylalaninemia newborn screening in the Special Administrative Region, China显示文摘Hyperphenylalaninemia 是在 15 000 实时出生影响约 1 的新陈代谢的最普通的先天的错误之一。在汉语之中, BH4 缺乏导致 hyperphenylalaninemia 比在白种人普通得多。准确诊断为治疗并且基因建议是重要的。在 2000,为苯酮尿的新生的屏蔽由在在整个整个国家的中国的法律是强制的。然而,它还没在香港特别行政区的新生的屏蔽节目被包括,中国。在香港汉语之中的 hyperphenylalaninemia 上的出版数据大部分正在缺乏。我们与严重 6-pyruvoyl-tetrahydropterin synthase (PTPS ) 报导一个 1 岁的香港中国女孩缺乏。病人与婴儿的 hypotonia 介绍了并且作为服的麻痹被误诊。她有的很温和的 hyperphenylalaninemia (95 mol/L ) ,显著地高的 phenylalnine-to-tyrosine 比率(3.1 ) ,和 1109 mlU/L 的提高的催乳激素。基因分析证实了同型结合的已知的引起疾病的变化磅 NM_000317.1:c.259CT;NP_000308.1:p。在 proband 的 P87S。根据我们的本地经验,当 PTPS 缺乏的 hyperphenylalaninemia 线索的估计的流行被报导在 29 542 实时出生,不是本氨基丙酸 hydroxylase 的一个单个案例是 1 时,缺乏被报导了。而且,有在社区以及在医药专业人员之中的继承新陈代谢的疾病的了解的一般缺乏。很经常,临床的怀疑的一个低索引将在诊断,多重不必要、昂贵的调查,延长病态和焦虑导致延期到影响的家庭。我们强烈建议为 hyperphenylalaninemia 屏蔽的那个扩展新生儿应该为在香港特别行政区出生的每个婴儿被实现,中国。 | Chloe Miu Mak Chun-hung Ko Ching-wan Lam Wai-ling Lau Wai-kwan Siu Sammy Pak-lam Chen Chun-yiu Law Chi-kong Lai Chak-man Yu Albert Yan-wo Chan | 2011 | Chinese Medical Journal2011，，16： | 2 |
| 14 | High rate DC-reactive sputter deposition of Y2 O3 film on the textured metal substrate for the superconducting coated conductor 显示文摘 | Kim Ho-Sup Park Chan Ko Rock-Kil Shi Dongqi Chung Jun-Ki Ha Hong-Soo Park Yu-Mi Song Kyu-Jeong Youm Do-Jun | 2005 | Physica C2005，426，431： | 1 |
| 15 | Effect of spinel content on hot strength of alumina-spinel castables in the temperature range 1 000~1 500 ℃显示文摘 | Ko Y C Chan C F | 1999 | Journal of the European Ceramic Society1999，19，15： | 1 |
| 16 | Prevalence of gestational diabetes mellitus in Hong Kong based on the 1998 WHO criteria显示文摘 | Ko GT Tam WH Chan JC | 2002 | Diabet Med2002，19，1： | 1 |
| 17 | Visualization of anterior chamber angle dynamics using optical coherence mography 显示文摘 | Leung C K Chan W M Ko C Y | 2005 | Ophthalmology2005，112，6： | 1 |
| 18 | Extracorporeal shockwave for hip necrosis in systemic lupus erythematosus显示文摘 | Wang CJ Ko JY Chan YS | 2009 | Lupus2009，18，12： | 1 |
| 19 | Electrical acustimulation of the wrist for chronic neck pain: a randomized, sham- controlled trial using a wrist - ankle acustimulation device显示文摘 | Chan DK Johnson MI Sun KO | 2009 | Clin J Pain2009，25，4： | 1 |
| 20 | Smoking and di- abetes in Chinesemen 显示文摘 | Ko GTC Chan JCN Tsang LWW | 2001 | Postgrad Med J2001，77，906： | 1 |
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