| 1 | Parkin-induced ubiquitination of Mff promotes its association with p62/SQSTM1 during mitochondrial depolarization显示文摘ubiquitin ligase Parkin 和 autophagic 改编者蛋白质 p62 被知道在控制 mitochondrial autophagy (mitophagy ) 的一条普通小径工作。然而,支持那 p62 被 ubiquitinated 蛋白质直接招募的证据仍然保持未经决定。这里,我们证明那个 mitochondrial 分裂因素(Mff ) 与 Parkin 联系,羰基氰化物 m-chlorophenyl hydrazone 处理显著地与 Mff 增加 Parkin 的亲密关系。在到动摇线粒体的招募以后, Parkin 调停在离氨酸 251 点的 Mff 的 poly-ubiquitination。由精氨酸(K251R ) 的离氨酸 251 的代替完全废除 Mff 的刺激 Parkin 的 ubiquitination。随后, ubiquitinated Mff 与 p62 支持它的协会。Mff 大美人防碍 p62 translocation 到损坏线粒体。仅仅 Mff WT 的 re-transfection,然而并非 K251R 异种,救这显型。而且, Mff 的损失导致 Parkin translocation 和损坏线粒体的最后的清理的失败。因此,我们的数据在 Mff, p62,和线粒体的选择 autophagy 之中揭示功能的连接,它在 neurodegeneration 疾病的致病被含有。 | Ju Gao | 2015 | Acta Biochimica et Biophysica Sinica2015，47，7： | 4 |
| 5 | Measures of bioavailable serum sex hormone levels in aging Chinese by protein chip显示文摘The purpose of this study was to develop a protein chip technique based on receptor binding assays to measure bioavailable serum sex hormone levels (BSSHL). 224 aging healthy Chinese were investigated to get the referenced values of BSSHL for the first time. In the assays recombined sex hormone receptor proteins were jointed to polysaccharide coated slides to make protein chip, and the dose-dependence curves of sex hormone on chip were prepared. The data showed that this method had good precision (CV<16%) and accuracy (Bias<10%), and the sensitivity could reach 1 pmol/L. From the results, BSSHL of men and women declined with aging, but no significant differences were observed. The BSSHL of aging men were higher than those of women. The bioavailable serum androgen level of men was 52―112 pmol/L, women's was 3―70 pmol/L and the whole group was 41.9―81.4 pmol/L. The bioavailable serum estrogen level of men was 0.8―3.0 pmol/L, women's was 1.2―2.5 pmol/L and the whole group was 0.6―2.64 pmol/L. Based on the assays, BSSHL measurement by protein chip can meet the needs of epidemiological studies in terms of speed, accuracy and sample volume required, and was helpful in quantitative assessment of aging people's health. | ZHOU Yong, CHANG Shuying, MENG Xiaoluo, YU Huafeng, WANG Luning, HE Jinggui, ZHANG Baohe, ZHANG Juntian, GENG Meiyu & DU Guanhua Institute of Marine Drugs and Food, Ocean University of China, Qingdao 266003, China Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China General Hospital of Air Force, PLA, Beijing 100036, China 304 Hospital of PLA, Beijing 100037, China Beijing Tongren Hospital, Beijing 100730, China General Hospital of PLA, Beijing 100853, China | 2006 | Science China(Life Sciences)2006，49，3： | 0 |
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