|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Mutation of the PHYTOENE DESATURASE 3 gene causes yellowish-white petals in Brassica napus显示文摘Oilseed rape (Brassica napus) with yellow flowers is an attractive ornamental landscape plant during the flowering period,and the development of different petal colors has become a breeding objective.Although yellowish flower color is a common variant observed in field-grown oilseed rape,the genetics behind this variation remains unclear.We obtained a yellowish-white flower (ywf) mutant from Zhongshuang 9 (ZS9) by ethyl methanesulfonate mutagenesis (EMS) treatment.Compared with ZS9,ywf exhibited a lower carotenoid content with a reduced and defective chromoplast ultrastructure in the petals.Genetic analysis revealed that the yellowish-white trait was controlled by a single recessive gene.Using bulked-segregant analysis sequencing (BSA-seq) and kompetitive allele-specific PCR(KASP),we performed map-based cloning of the ywf locus on chromosome A08 and found that ywf harbored a C-to-T substitution in the coding region,resulting in a premature translation termination.YWF,encoding phytoene desaturase 3 (PDS3),was highly expressed in oilseed rape petals and involved in carotenoid biosynthesis.Pathway enrichment analysis of the transcriptome profiles from ZS9 and ywf indicated the carotenoid biosynthesis pathway to be highly enriched.Further analyses of differentially expressed genes and carotenoid components revealed that the truncated Bna A08.PDS3 resulted in decreased carotenoid biosynthesis in the mutant.These results contribute to an understanding of the carotenoid biosynthesis pathway and manipulation of flower-color variation in B.napus. | Chuanji Zhao Luqman Bin Safdar Meili Xie Meijuan Shi Zhixue Dong Li Yang Xiaohui Cheng Yueying Liu Zetao Bai Yang Xiang Chaobo Tong Junyan Huang Lijiang Liu Shengyi Liu | 2021 | The Crop Journal2021，9，5： | 3 |
| 2 | Syntenic quantitative trait loci and genomic divergence for Sclerotinia resistance and ?owering time in Brassica napus显示文摘Oilseed rape(Brassica napus) is an allotetraploid with two subgenomes descended from a common ancestor. Accordingly, its genome contains syntenic regions with many duplicate genes, some of which may have retained their original functions, whereas others may have diverged. Here, we mapped quantitative trait loci(QTL) for stem rot resistance(SRR), a disease caused by the fungus Sclerotinia sclerotiorum, and flowering time(FT) in a recombinant inbred line population. The population was genotyped using B.napus 60 K single nucleotide polymorphism arrays and phenotyped in six(FT) and nine(SSR) experimental conditions or environments. In total, we detected 30 SRR QTL and 22 FT QTL and show that some of the major QTL associated with these two traits were co-localized,suggesting a genetic linkage between them. Two SRR QTL on chromosome A2 and two on chromosome C2 were shown to be syntenic, suggesting the functional conservation of these regions. We used the syntenic properties of the genomic regions to exclude genes for selection candidates responsible for QTL-associated traits. For example, 152 of the 185 genes could be excluded from a syntenic A2–C2 region. These findings will help to elucidate polyploid genomics in future studies, in addition to providing useful information for B. napus breeding programs. | Fengqi Zhang Junyan Huang Minqiang Tang Xiaohui Cheng Yueying Liu Chaobo Tong Jingyin Yu Tehrim Sadia Caihua Dong Lingyan Liu Baojun Tang Jianguo Chen Shengyi Liu | 2019 | Journal of Integrative Plant Biology2019，61，1： | 3 |
| 3 | Mapping loci controlling fatty acid profiles, oil and protein content by genome-wide association study in Brassica napus显示文摘Optimizing the profile and quantity of fatty acids in rapeseed(Brassica napus L.) is critical for maximizing the value of edible oil and biodiesel. However, selection of these complex seed quality traits is difficult before haplotypes controlling their contents are identified. To efficiently identify genetic loci influencing these traits and underlying candidate genes and networks, we performed a genome-wide association study(GWAS) of eight seed quality traits(oil and protein content, palmitic, stearic, oleic, linoleic, eicosenoic and erucic acids content). The GWAS population comprised 370 diverse accessions, which were phenotyped in five environments and genotyped using 60K SNP arrays. The results indicated that oil and protein contents generally showed negative correlations, while fatty acid contents showed positive or negative correlations,with palmitic and erucic acid contents directly affecting oil content. Seven SNPs on five chromosomes were associated with both seed oil and protein content, and five genes orthologous to genes in Arabidopsis thaliana were predicted as candidates. From resequencing data, besides known haplotypes in Bna A.FAE1.a and Bna C.FAE1.a, three accessions harboring a new haplotype conferring moderate erucic acid content were identified. Interestingly, in a haplotype block, one haplotype was associated with high palmitic acid content and low oil content, while the others showed the reverse effects. This finding was consistent with a negative correlation between palmitic acid and oil contents, suggesting historical selection for high oil content. The identification by this study of genetic variation and complex correlations of eight seed quality traits may be beneficial for crop selection strategies. | Minqiang Tang Yuanyuan Zhang Yueying Liu Chaobo Tong Xiaohui Cheng Wei Zhu Zaiyun Li Junyan Huang Shengyi Liu | 2019 | The Crop Journal2019，7，2： | 1 |
| 4 | Cloning and sequence analysis of Sox genes in a tetraploid cyprinid fish,Tor douronensis显示文摘A PCR survey for Sox genes in a young tetraploid fish Tor douronensis (Teleostei:Cyprinidae) was per-formed to access the evolutionary fates of important functional genes after genome duplication caused by polyploidization event. Totally 13 Sox genes were obtained in Tor douronensis,which represent SoxB,SoxC and SoxE groups. Phylogenetic analysis of Sox genes in Tor douronensis provided evidence for fish-specific genome duplication,and suggested that Sox19 might be a teleost specific Sox gene member. Sequence analysis revealed most of the nucleotide substitutions between duplicated copies of Sox genes caused by tetraploidization event or their orthologues in other species are silent substitutions. It would appear that the sequences are under purifying selective pressure,strongly suggesting that they repre-sent functional genes and supporting selection against all null allele at either of two duplicated loci of Sox4a,Sox9a and Sox9b. Surprising variations of the intron length and similarities of two duplicated copies of Sox9a and Sox9b,suggest that Tor douronensis might be an allotetraploidy. | GUO BaoCheng Li JunBing TONG ChaoBo HE ShunPing | 2008 | Chinese Science Bulletin2008，53，13： | 1 |
| 5 | BnaOmics:A comprehensive platform combining pan-genome and multi-omics data from Brassica napus显示文摘Dear Editor,Brassica napus was originally formed7500 years ago by the interspecific hybridization of B.rapa and B.oleracea(Chalhoub et al.,2014).It accounts for approximately 13%–16%of global vegetable oil production and provides an excellent model for polyploid genomics and evolutionary research in plants.Currently,the Brassicaceae Database(BRAD V3.0;Chen et al.,2022),Genoscope(https://www.genoscope.cns.fr/brassicanapus/),and Ensembl Plants(https://plants.ensembl.org)are used for genomic research pertaining to B.napus.However,they only provide genome browsers for research into the B.napus cultivar Darmorbzh.The B.napus pan-genome information resource(BnPIR)also provides eight B.napus reference genomes(Song et al.,2021),gene information,and resequencing data.The B.napus variation information resource(BnVIR)provides information on genetic variation,including single-nucleotide polymorphisms(SNPs),insertions or deletions(INDELs),and structural variations(SVs)(Yang et al.,2022). | Xiaobo Cui Ming Hu Shengli Yao Yuanyuan Zhang Minqiang Tang Lijiang Liu Xiaohui Cheng Chaobo Tong Shengyi Liu | 2023 | Plant Communications2023，4，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员