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| 1 | 油棕细胞悬浮培养及植株再生技术显示文摘【目的】建立油棕胚性体细胞悬浮培养植株再生技术体系,为油棕优株无性系种植材料高效繁育、细胞生物学研究、生物技术育种研究等提供技术平台和参考。【方法】以源于成龄油棕芯叶的瘤状愈伤组织为材料,采用不同质量浓度2,4-D、麦草畏与德夸霉素组合诱导易碎胚性愈伤组织,分析不同激素质量浓度及其组合对油棕体细胞悬浮系增殖与后续体胚发育的影响。【结果】0.1 mg/L德夸霉素与一定浓度2,4-D或麦草畏组合,促进胚性愈伤组织形成,0.1 mg/L德夸霉素与1 mg/L麦草畏组合诱导易碎胚性愈伤组织较佳,诱导率为28.67%;用生长旺盛、质地松散的易碎胚性愈伤组织悬浮培养21 d可建立生长状态较均一、分散性好的悬浮细胞系;0.3 mg/L德夸霉素与1 mg/L麦草畏组合较有利于悬浮细胞系增殖和后续体胚发育;将悬浮细胞系转到MS+谷氨酰胺100 mg/L +蔗糖30g/L培养基上,于28(±2)℃、2 000 lx下进行胚诱导培养,每两周更换新培养基1次,培养60 d可获得直径大于1 mm的胚,最高每瓶295粒;将大于1 mm的胚转到固体培养基培养可获得再生植株。【结论】适当质量浓度德夸霉素与2,4-D或麦草畏组合,促进油棕芯叶瘤状愈伤组织形成易碎胚性愈伤组织;用生长旺盛、质地松散的易碎胚性愈伤组织悬浮培养21 d可建立生长状态较均一、分散性好的悬浮细胞系;初步发现含适当质量浓度德夸霉素与麦草畏液体培养基利于悬浮细胞系增殖和后续体胚发育。 | 潘登浪 邹积鑫 曾宪海 林位夫 Khengtuan Chean | 2019 | 广东农业科学2019，46，2： | 3 |
| 2 | a series of 182 repairs 显示文摘 | Chean WK So JB Lomanto D Endoscopic extraperitoneal inguinal hernia repair | 2004 | Singapore Med J2004，45，： | 1 |
| 3 | Substance P and vasoactive intestinal peptide in nasal sections and plasma from patients with nasal allergy显示文摘 | Chean T Watanabe N Mogi G | 1993 | Ann Otol Rhinol Laryngol1993，102，： | 1 |
| 4 | Basal-like breast cancer defined by five biomakers has superior prognostic value than triple-negative pheno- type显示文摘 | Chean MC Voduc D Bajdik C | 2008 | Clin Cancer Res2008，14，5： | 1 |
| 5 | 2 - HYdroxypropyl - beta - cyclodextrin increases aqueous solubility and photostability of all-trans-retinoic acid 显示文摘 | Lin HS Chean CS Ng YY | 2000 | J Chin Pharm Ther2000，25，4： | 1 |
| 6 | Cure kinetics and morphology of amine-cured tetraglycidyl-4,4′-diaminodiphenylmethane epoxy blends with poly(ether imide)显示文摘 | Chean C. Su Eamor M. Woo | 1995 | Polymer1995，，15： | 1 |
| 7 | Aboradband UHF near-field RFID antenna 显示文摘 | QING Xian-ming GOH Chean Khan CHEN Zhi-ning | 2010 | IEEE Transaction on Antennas and Propagation2010，58，12： | 1 |
| 8 | Endoscopic extraperitoneal ingu- inal hernia repair a series of 182 repairs显示文摘 | Chean WK So JB Lomanto D | 2004 | Singapore Med J2004，45，6： | 1 |
| 9 | 2-Hydroxypropyl-β-cyclodextrin increases aqueous solubility and photostability of all-trans-retinoic acid显示文摘 | Lin HS Chean CS Ng YY | 2000 | J Clin Pharm Ther2000，25，4： | 1 |
| 10 | Activity Recognition using Visual Tracking and RFID显示文摘 | Krahnstoever N Rittscher J Chean K | | 0，，： | 1 |
| 11 | Protective effect of a mixture of Aloe vera and Silybum marianum against carbon tetraehloride - in- duced acute hepatotoxieity and liver fibrosis 显示文摘 | Kim SH Chean HI Yun N | 2009 | J Pharmaeol Sei2009，109，： | 1 |
| 12 | 2-hydroxypropyl-b cyclodextrin increases aqueous solubility and photostability of all-trans-retinoic acid 显示文摘 | Lin HS Chean CS Ng YY | 2000 | J Clin Pharm Ther2000，25，4： | 1 |
| 13 | Cure kinetics and morphology of amine-cured 4,4-diamiodiphenylmethane epoxy blends with poly(ether inide)显示文摘 | Chean C Su Eamor M Woo | 1995 | Polymer1995，36，15： | 1 |
| 14 | Deep sclerectomy-trabeculectomy with mitomycin C in treating glaucoma:postoperative long-term results显示文摘AIM:To determine the long-term postoperative outcomes of deep sclerectomy-trabeculectomy(DST)with mitomycin C(MMC)in the treatment of glaucoma.METHODS:Patients who underwent DST with MMC between 2010 and 2017 were included in this retrospective observational study.Complete success was defined as postoperative intraocular pressure(IOP)≤21 mm Hg or 30%reduction of IOP from baseline without any topical IOP-lowering agent,and qualified success defined as IOP≤21 mm Hg or 30%reduction of IOP from baseline with/without single topical agent.We evaluated the surgical success rates and complication rates of this procedure,as well as described the IOP profiles,best corrected visual acuity(BCVA)profiles and mean deviations(MD)of Humphrey visual field(HVF)24-2 performance at each follow-up time point.Mixed linear regression models were constructed to determine estimated predictive values of demographic data,use of topical IOPlowering agents,baseline and postoperative IOP and optical profiles(e.g.,BCVA and MD).RESULTS:Totally 98 eyes(mean postoperative followup 67.5mo)showed mean IOP reduction at every followup interval.Both median BCVA and MD of visual fields were maintained throughout the follow-up intervals when comparing to baseline.The number of IOP-lowering medications decreased from 2.8±0.8 to 0.3±0.7(P=0.068).Totally 84(85.7%)eyes achieved complete success at final follow-up.Transient hyphaema and transient choroidal effusion developed in 15 eyes(15.3%)and 11 eyes(11.2%)respectively.Other complications included shallow anterior chamber in 5 eyes(5.1%),bleb leak in 4 eyes(4.1%),bleb revision in 7 eyes(7.1%),bleb needling in 9 eyes(9.2%)and repeat trabeculectomy in 1 eye(1.0%).There was no endophthalmitis,blebitis or macular oedema.There was no significant correlation between postoperative IOP control and postoperative BCVA.CONCLUSION:DST with MMC demonstrates effective and sustained long-term outcomes in the treatment of glaucoma with no major complication. | Chung Shen Chean Li Jiang Gayathri Kanchana Niroshani Pati Arambage Punithawathy Ranjit | 2022 | International Journal of Ophthalmology(English edition)2022，15，12： | 1 |
| 15 | Cure kinetics and interdomain etherifieation in an amine cured phenoxy/epoxy system显示文摘 | Hseih H K Chean C S Woo E M | 1998 | Polymer1998，39，11： | 1 |
| 16 | 2-Hydroxypropyl-beta-cyclodeain increases aqueous solubility and photostebility of all-transretinoic acid显示文摘 | LIN H S CHEAN C S NG Y Y | 2000 | J Chin Pharm Ther2000，25，6： | 1 |
| 17 | Endoscopic extraperltoneal inguinal hernia repair a series of 182 repairs显示文摘 | Chean WK So JB Lomanto D | 2004 | Singapore Med J2004，45，6： | 1 |
| 18 | Basal-like breast cancer defined by five biomarkers has superior prognostic value than triple-negative phenotype显示文摘 | Chean M C Voduc D Bajdik C | 2008 | Clin Cancer Res2008，14，5： | 1 |
| 19 | Endoscopic extraperitoneal inguinal hernia repair,a series of 182 repairs显示文摘 | Chean W K So J B Lomanto D | 2004 | Singapore Med J2004，45，： | 1 |
| 20 | Experimental characterization of interfacial adhesion of an optical fiber embedded in a composite material显示文摘 | V. Chean R. Matadi Boumbimba R. El Abdi J.C. Sangleboeuf P. Casari M. Drissi Habti | 2013 | International Journal of Adhesion and Adhesives2013，，： | 1 |
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