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| 1 | Immunization with chlamydial plasmid protein pORF5 DNA vaccine induces protective immunity against genital chlamydial infection in mice显示文摘To validate the immune protective efficacy of pORF5 DNA vaccine and to analyze potential mechanisms related to this protection. In this study, pORF5 DNA vaccine was constructed and evaluated for its protective immunity in a mouse model of genital chlamydial infection. Groups of BALB/c mice were immunized intranasally with pORF5 DNA vaccine. Humoral and cell mediated immune responses were evaluated. The clearance ability of chlamydial challenge from the genital tract and the chlamy- dia-induced upper genital tract gross pathology and histopathological characterization were also de- tected. The results showed that the total and the IgG2a anti-pORF5 antibody levels in serum were sig- nificantly elevated after pcDNA3.1-pORF5 vaccination, as were the total antibody and IgA levels in vaginal fluids. pcDNA3.1-pORF5 induced a significantly high level of Th1 response as measured by robust gamma interferon (IFN-γ). Minimal IL-4 was produced by immune T cells in response to the re-stimulation with pORF5 protein or the inactive elementary body in vitro. pcDNA3.1-pORF5-vacci- nated mice displayed significantly reduced bacterial shedding upon a chlamydial challenge and an accelerated resolution of infection. 100% of pcDNA3.1-pORF5 vaccinated mice successfully resolved the infection by day 24. pcDNA3.1-pORF5-immunized mice also exhibited protection against patho- logical consequences of chlamydial infection. The stimulated index was significantly higher than that of mice immunized with pcDNA3.1 and PBS (P<0.05). Together, these results demonstrated that immu- nization with pORF5 DNA vaccine is a promising approach for eliciting a protective immunity against a genital chlamydial challenge. | LI ZhongYu1,2, WANG ShiPing1, Wu YiMou2, ZHONG GuangMing3 & CHEN Ding3 1 Xiangya School of Medicine, Central South University, Changsha 410078, China 2 Department of Microbiology and Immunology, University of South China, Hengyang 421001, China 3 University of Texas Health Science Center at San Antonio, TX 78229, USA | 2008 | Science China(Life Sciences)2008，51，11： | 16 |
| 2 | Rational holomorphic maps from B^2 into B^N with degree 2显示文摘Rational proper holomorphic maps from the unit ball in C2 into the unit ball CN with degree 2 are studied. Any such map must be equivalent to one of the four types of maps. | CHEN Zhihua, Jl Shanyu & XU Dekang Department of Mathematics, Tongji University, Shanghai 200092, China Department of Mathematics, University of Houston, Houston, TX 77204, USA | 2006 | Science China Mathematics2006，49，11： | 3 |
| 3 | Myelin activates FAK/Akt/NF-kappa B pathways and provokes CR3-dependent inflammatory response in murine system显示文摘 | Sun X Wang X Chen TX | 2010 | Plos One2010，5，2： | 1 |
| 4 | Regulation of the potentialmarker for intestinal cells,Bmil,by β-catenin and the zincfingerprotein KLF4:implications for colon cancer显示文摘 | Yu TX Chen X Zhang W | 2012 | J Biol Chem2012，287，6： | 1 |
| 5 | Myelin activates FAK/Akt/NF-kB pathways and provokes CR3-dependent inflammatory response in murine system显示文摘 | Sun X Wang X Chen TX | 2010 | PLoS ONE2010，5，2： | 1 |
| 6 | Roles of mTOR and p-mTOR in gastro- intestinal stromal tumors显示文摘 | Li JC Zhu HY Chen TX | 2013 | Asian Pac J Cancer Prev2013，14，10： | 1 |
| 7 | Technique and problem of ERCP diagnosis bile duct carinoma 显示文摘 | ZHANG GJ CHEN TX | 1996 | Zhongguo Neijing Zazhi1996，2，3： | 1 |
| 8 | Clinical and molecular characteristics of 35 Chinese children with Wiskott-Aldrich syndrom显示文摘 | Lee PP Chen TX Jiang LP | 2009 | J Clin Immunol2009，29，4： | 1 |
| 9 | Left ventricular dysfunction and associated cellular injury in rats exposed to chronic intermittent hypoxia 显示文摘 | Chen L Zhang J Gan TX | 2008 | J Appl Physiol2008，104，1： | 1 |
| 10 | Structure activity relationshipof natural flavonoids inhydroxyl radicai scavenging effects显示文摘 | Chen JW Zhu ZQ Hu TX | 2002 | Acta Pharmacologica Sinica2002，23，7： | 1 |
| 11 | Mir-144 selectively regulates embryonic alpha-hemoglobin synthesis during primitive erythropoiesis 显示文摘 | Fu YF Du TF Dong M Zhu KY Jing CB Zhang Y Wang L Fan HB Chert Y Jin Y Yue GP Chen S J Chen Z Huang QH Jing Q Deng M Liu TX | 2009 | Blood 20092009，134，： | 1 |
| 12 | Clinical characteristics and genotype-phenotype correlation in 62 patients with X-linked agammaglobulinemia显示文摘 | Lee PP Chen TX Jiang LP | 2010 | J Clin Immunol2010，30，： | 1 |
| 13 | The additive erectile recovery effect of brain-derived neurotrophic factor combined with vascular endothelial growth factor in a rat model of neurogenic impotence显示文摘 | Chen KC Minor TX Rahman NU | 2005 | BJU Int2005，95，7： | 1 |
| 14 | The additive erectile recovery effect of brain-derived neurotrophic factor combined with vascular endothelial growth factor in a rat model of neurogenic impotence显示文摘 | Chen KC Minor TX Rahman NU | | BJU Int0，95，7： | 1 |
| 15 | Left ventricular dysfunction and associated cellular injury in rats exposed to chronic intermittent hypoxia显示文摘 | Chen L Zhang J Gan TX | 2008 | J Appl Physiol ( 1985)2008，104，： | 1 |
| 16 | Left ventrieular dysfunc-tion and associated cellular injury in rats exposed to chronic intermittent hypoxia 显示文摘 | Chen L Zhang J Gan TX | 2008 | J Appl Physiol2008，104，1： | 1 |
| 17 | Left ventricular dysfunction and associated cellular injury in rats exposed to chronic intermittent hypoxia显示文摘 | Chen L Zhang J Gan TX | 2008 | J Appl Physiol (1985)2008，104，1： | 1 |
| 18 | The truncated Second Main Theorem and uniqueness theorems显示文摘In this paper, we first establish a truncated Second Main Theorem for algebraically nondegenerate holomorphic mappings from the complex plane into a complex projective variety V intersecting hypersurfaces. We then prove some uniqueness results for meromorphic mappings. The result of Demailly about a partial solution to the Fujita’s conjecture is used. | CHEN ZhiHua 1 , RU Min 2, & YAN QiMing 1 1 Department of Mathematics, Tongji University, Shanghai 200092, China 2 Department of Mathematics, University of Houston, Houston, TX 77204, USA | 2010 | Science China Mathematics2010，53，3： | 1 |
| 19 | The additive erectile recovery effect of brain-derived neurotrophic factor combined with vascular endothelial growth factor in a rat model of neurogenic impotence显示文摘 | Chen KC Minor TX Rahman NU | 2005 | BJU Int2005，95，7： | 1 |
| 20 | Topotecan for ovarian cancer 显示文摘 | Peng LH Chen XY Wu TX | 2007 | The Cochrane Database of Systematic Reviews2007，，00： | 1 |
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