| 2 | Expression of Interleukin—10 mRNA in Children with Different Clinical Types of Henoch—Schonlein Purpura Nephritis显示文摘Objective To investigate the role of interleukin-10(IL-10) in the pathogenesis of different clinical types Henoch-Schonlein purpura nephritis(HSPN).Methods The IL-10 protein was determined by ELISA and the expression of IL-10 mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR) in phytohemagglutinin(PHA)-stimulating peripheral blood mononuclear cells(PBMCS) in 40 children with HSPN during their acute phase and remission stage.Results:(1)The levels of IL-10 mRNA and protein were significantly increased in patients in acute phase compared with controls[(0.48±0.12)vs(0.39±0.10)and(1023.90±158.78)pg/ml vs(895.51±141.06)pg/ml;P<0.01 and 0.05 respectively],while in remission stage the levels of IL-10 decreased to normal levels[(0.42±0.13)vs(0.39±0.10)and(941.25±165.36)pg/ml vs(895.51±141.06)pg/ml;P>0.05 for both].(2)The IL-10 mRNA and protein of HSPN patients in acute phase with proteinuria,nephrotic syndrome or acute glomerulonephritis syndrome was signficantly higher than that in controls.There was no significant difference between the acute phase of HSPN patients with isolated hematuria and the controls.Conclusion The results suggested that the expression of IL-10 in PBMC was correlated to clinical type of HSPN.IL-10 has protective action in the pathogenesis of HSPN. | WuYJ ChenRH | 2002 | Journal of Nanjing Medical University2002，16，1： | 0 |
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