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| 1 | Chemical conjugation of a novel antibody interleukin 2 immunoconjugate against c erbB 2 product显示文摘Objective To develop a new chemical method to produce a monoclonal antibody (MoAb) 520C9/recombinant human interleukin 2 (rhIL 2) conjugate Methods MoAb 520C9 reactive with the protooncogene c erbB 2 product P185 was chemically conjugated with rhIL 2 by using a simple two step method First, the rhIL 2 was activated by Sulfosuccinimidyl 4 [N maleimidomethyl] cyclohexane 1 carboxylate, a heterobifunctional linker, and N succinimidyl s acetylthioacetate was introduced onto 520C9 Then SATA on the 520C9 was reacted with the maleimide group on the activated rhIL 2 to generate 520C9 rhIL 2 immunoconjugate Results The immunoconjugate retained the antigen binding activity compared to the respective native antibody as determined by an indirect live cell binding assay The immunoconjugate also possessed IL 2 activity as measured by the standard CTLL 2 cells proliferation assay and the stimulation of human peripheral blood mononuclear cells (PBMCs) into lymphokine activated killer cells Conclusion Our method of conjugation of rhIL 2 to 520C9 preserves the binding activity of the antibody and the cytokine function of IL 2 This simple and efficient method of conjugation should be applicable to other types of MoAbs and recombinant | 李君 Sharon E Lee Marie Belciug David B Ring Cheuk S Kwok | 2000 | Chinese Medical Journal2000，，2： | 3 |
| 2 | Gamma knife surgery for trigeminal neuralgia:Outcome,imaging,and brainstem correlates显示文摘 | Cheuk A V Chin L S Petit J H | 2004 | International Journal of Radiation Oncology Biology Physics2004，60，2： | 1 |
| 3 | Pipe-soil interaction behaviour during lateral buckling显示文摘 | BRUTON D A S WHIhite D J CHEUK C Y | 2006 | SPE Projects Facilities & Construction2006，1，3： | 1 |
| 4 | Instability caused by aseepage impediment in layered fill slopes 显示文摘 | LEE Y S CHEUK C Y SASSA K | 2008 | Cannada Geotech2008，45，： | 1 |
| 5 | 显示文摘 | Cheuk K K L Li B S Lam J W Y | 2008 | Macromolecules2008，41，： | 1 |
| 6 | Immunostaining for thyroid transcription factor 1 and cytokeratin 20 aids the distinction of small cell carcinoma from Merkel cell carcinoma, but not pulmona- ry from extrapulmonary small cell carcinoma 显示文摘 | Cheuk W Kwan M Suster S | 2001 | Arch Pathol I ab Med2001，125，2： | 1 |
| 7 | Annexin A1 expression in atherosclerotic carotid plaques and its relationship with plaque characteristics显示文摘 | Cheuk B L Cheng S W | 2011 | Eur J Vasc Endovasc Surg2011，41，3： | 1 |
| 8 | Epidermal Th22 andTcl7 cells form a localized disease memory in clinicallyhealed psoriasis显示文摘 | Cheuk S Wiken M Blomqvist L | 2014 | J Immunol2014，192，7： | 1 |
| 9 | Can local secretion of prostaglandin E2, thromboxane B2, and interleukin-6 play a role in ruptured abdominal aortic aneurysm? 显示文摘 | CHEUK B L CHENG S W | | World J Surg0，32，1： | 1 |
| 10 | Apple polyphenols inhibit plasma CETP activity andreduce the ratio of non-HDL to HDL cholesterol显示文摘 | CHEUK KAI LAM ZHANG Z S YU H J | 2008 | Nutr Food Res2008，52，： | 1 |
| 11 | Annexin A1expression in atherosclerotic carotid plaques and its relationship with plaque characteristics显示文摘 | CHEUK B L CHENG S W | 2011 | Eur J Vasc Endovasc Surg2011，41，： | 1 |
| 12 | Atypical epithelial proliferations in acquired renal cystic disease harbor eytogenetic aberrations 显示文摘 | CHEUK W LO E S CHAN A K | 2002 | Hum Pathol2002，33，： | 1 |
| 13 | Identification of FGFR4-activating mutations in human rhabdomyosarcomas that promote metastasis in xenotransplanted models显示文摘 | Taylor James G Cheuk Adam T Tsang Patricia S Chung Joon-Yong Song Young K Desai Krupa Yu Yanlin Chen Qing-Rong Shah Kushal Youngblood Victoria Fang Jun Kim Su Young Yeung Choh Helman Lee J Mendoza Arnulfo Ngo Vu Staudt Louis M Wei Jun S | 2009 | Journal of Clinical Investigation2009，，11： | 1 |
| 14 | Synthesis, chain helicity, assembling structure, and biological compatibility of poly (phenylacetylene) s containing /-alanine moieties 显示文摘 | CHEUK K K L LIB S LAM J W Y | 2008 | Macromolecules2008，41，14： | 1 |
| 15 | Immunostaining for human herpesvirus 8 latent nuclear antigen-1 helps distinguish kaposi sarcoma from its mimickers 显示文摘 | Cheuk W Wang K O Wong C S | 2004 | Am J Clin Pathol2004，121，3： | 1 |
| 16 | Consistent Immunostaining for Cyclin D1 Can Be Achieved on a Aoutine Basis Using a Newly Available Rabbit Monoclonal Antibody显示文摘 | Cheuk W Wong K O Wong C S | 2004 | Am J Surg Pathol2004，28，6： | 1 |
| 17 | Identification of FGFR4-activating mutations in human rhabdomyosarcomas that promote metastasis in xenotransplanted models显示文摘 | Taylor J G Cheuk A T Tsang P S | 2009 | J Clin Invest2009，119，： | 1 |
| 18 | Immunostain- ing for thyroid transcription factor 1 and cytokeratin 20 aids the distinction of small cell carcinoma from Merkel cell carcinoma,but not pulmonary from extrapulmonary small cell carcinomas显示文摘 | Cheuk W Kwan M Y Suster S | 2001 | Arch Pathol Lab Med2001，125，2： | 1 |
| 19 | Synthesis and Hierarchical Structures of Amphiphilic Polyphenylacetylenes Carrying L-Valine Pendants显示文摘 | LI B S CHEUK K K L LING L | 2003 | Macromolecules2003，36，： | 1 |
| 20 | Immunostaining for thyroid transcription factor 1 and cytokeratin 20 aids the distinction of small cell carcinoma from Merkel cell carcinoma,but not pulmonary from extrapulmonary small cell carcinomas显示文摘 | Cheuk W Kwan M Y Suster S Chan J K | 2001 | Arch Pathol Lab Med2001，125，2： | 1 |
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