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| 1 | Establishment and characterization of an opisthorchiasis-associated cholangiocarcinoma cell line (KKU-100)显示文摘AIM To establish and dharacterize a nev cholangiocarcinoma cell line from a patient living in the Opisthorchis viverrini (O. viverrini) endemic area of Northeast Thailand.METHODS: Fresh liver biopsy and bile specimens were obtained from a 65-year-old Thai woman with cholangiocarcinoma of the porta hepatis. After digestion, the cells were cultured in Ham's F12 media. The established cell line was then characterized for growth kinetics, cell morphology, imm unocytochemistry and cytogenetics. Tumorigenicity of the cell line was determined by heterotransplanting in nude mice. RESULTS: The primary tumor was a poorly differentiated tubular adenocarcinoma. Examination of the bile revealed malignant cells with O. viverrini eggs. The cholangiocarcinoma cell line KKU-100 was established 4 mo after the primary culture-population doubling time was 72 h. KKU-100 possesses compact and polygonal-shapedepithelial cells. Immunocytochemically, this cell line exhibited cytokeratin, EMA, CEA, and CA125, but not α-fetoprotein (AFP), CA19-9, desmin, c-met, or p53. Such protein expressions parallel those of the primary tumor. Cytogenetic analysis identified aneuploidy karyotypes with a modal chromosome number of 78 and marked chromosomal structural changes. Inoculation of KKU-100 cells into nude mice produced a transplantable, poorly differentiated aden-ocarcinoma, similar to the original tumor.CONCLUSION: KKJ-100 is the first egg-proven, Opisthorchis- associated cholangiocarcinoma cell line, which should prove useful for further investigations of the tumor biology of this cancer. | Banchob Sripa Saman Leungwattanawanit Takayuki Nitta Chaisiri Wongkham Vajarabhongsa Bhudhisawasdi Anucha Puapairoj Chongrak Sripa Masanao Miwa | 2005 | World Journal of Gastroenterology2005，11，22： | 4 |
| 2 | Role of plant uptake on nitrogen removal in constructed wetlands located in the tropics显示文摘 | Thammarat Kootaatep Chongrak Polprasert | 1997 | Water Sci Technol1997，36，12： | 1 |
| 3 | Hazardous waste generation and processing显示文摘 | CHONGRAK P LIYANAGE L R J | 1996 | Resources Conservation and Recycling1996，16，： | 1 |
| 4 | Evaluation of phosphate fertilizers for the stabilization of cadmium in highly contaminated soils显示文摘 | USARAT THAWORNCHAISIT CHONGRAK POLPRASERT | 2009 | Journal of Hazardous Materials2009，165，： | 1 |
| 5 | Nitrogen mass balance and microbial analysis of constructed wetlands treating municipal landfill leachate显示文摘 | Variga Sawaittayothin Chongrak Polprasert | 2006 | Bioresource Technology2006，，3： | 1 |
| 6 | New POPs in the water environment: distribution, bioaccu mulation and treatment of perfluorinated compounds - a review paper 显示文摘 | Fujii Shigeo Chongrak Polprasert Tanaka Shuhei | 2007 | J Water Supply2007，56，5： | 1 |
| 7 | Nitrogen mass balance and microbial analysis of constructed wetlands treating municipal landfill leachate 显示文摘 | Variga Sawaittayothin Chongrak Polprasert | 2007 | Bioresource Technology2007，98，： | 1 |
| 8 | Inheritance of seed phytate in mungbean ( Vigna radiata (L.) Wilczek)显示文摘 | Utumporn Sompong Chongrak Kaewprasit Sutkhet Nakasathien Peerasak Srinives | 2010 | Euphytica2010，，3： | 1 |
| 9 | Nitrogen mass balance and microbial analysis of constructed wetlands treating municipal landfill leachate显示文摘 | VARIGA SAWAITTAYOTHIN CHONGRAK POLPRASERT | 2007 | Bioresource Technology2007，，3： | 1 |
| 10 | Phosphorus recovery from human urine and anaerobically treated wastewater through pH adjustment and chemical precipitation显示文摘 | Patiya Kemacheevakul Chongrak Polprasert Yoshihisa Shimizu | 2011 | Environmental Technology2011，，7： | 1 |
| 11 | Abundance of amoA genes of ammonia-oxidizing archaea and bacteria in activated sludge of full-scale wastewater treatment plants显示文摘 | Tawan Limpiyakorn Puntipar Sonthiphand Chaiwat Rongsayamanont Chongrak Polprasert | 2010 | Bioresource Technology2010，，4： | 1 |
| 12 | Nitrogen mass balance and microbial analysis of constructed wetlands treating municipal landfill leachate 显示文摘 | Variga Sawaittayothin Chongrak Polprasert | 2007 | Bioresource Technology2007，98，3： | 1 |
| 13 | Effluent denitrification with anaerobic filters显示文摘 | Chongrak Polprasert H S Park | 1986 | Wat Res1986，58，8： | 1 |
| 14 | Heavy metal pollution in the Chao Phraya River estuary,Thailand显示文摘 | Chongrak P | 1982 | Marine Pollution Bulletin1982，16，： | 1 |
| 15 | Influence of condensed silica fume on the properties of cement-based solidified wastes显示文摘 | Suwimol Asavapisit Weena Nanthamontry Chongrak Polprasert | 2001 | Cement and Concrete Research2001，，8： | 1 |
| 16 | An integrated kinetic model for organic and nutrient removal by duckweed-based wastewater treatment (DUBWAT) system显示文摘 | K.C. Bal Krishna Chongrak Polprasert | 2008 | Ecological Engineering2008，，3： | 1 |
| 17 | Nitrogen mass balance and microbial analysis of constructed wetlands treating municipal landfill leachate 显示文摘 | Variga S Chongrak P | 2007 | Bioresource Technology2007，98，3： | 1 |
| 18 | Evaluation of phosphate fertilizers for the stabilization of cadmium in highly contaminated soils显示文摘 | Usarat Thawornchaisit Chongrak Polprasert | 2008 | Journal of Hazardous Materials2008，，1： | 1 |
| 19 | Septage disposal in waste recycling ponds显示文摘 | Chongrak P S Udom K H Choidry | 1984 | Water Research1984，18，5： | 1 |
| 20 | Evalu-ation of phosphate fertilizers for the stabilization ofcadmium in highly contaminated soils 显示文摘 | Usarat Thawomchaisita Chongrak Polprasertb | 2009 | J HazardMater2009，165，123： | 1 |
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