|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Super-resolution localization photoacoustic microscopy using intrinsic red blood cells as contrast absorbers显示文摘Photoacoustic microscopy(PAM)has become a premier microscopy tool that can provide the anatomical,functional,and molecular information of animals and humans in vivo.However,conventional PAM systems suffer from limited temporal and/or spatial resolution.Here,we present a fast PAM system and an agent-free localization method based on a stable and commercial galvanometer scanner with a custom-made scanning mirror(L-PAM-GS).This novel hardware implementation enhances the temporal resolution significantly while maintaining a high signal-to-noise ratio(SNR).These improvements allow us to photoacoustically and noninvasively observe the microvasculatures of small animals and humans in vivo.Furthermore,the functional hemodynamics,namely,the blood flow rate in the microvasculature,is successfully monitored and quantified in vivo.More importantly,thanks to the high SNR and fast B-mode rate(500 Hz),by localizing photoacoustic signals from captured red blood cells without any contrast agent,unresolved microvessels are clearly distinguished,and the spatial resolution is improved by a factor of 2.5 in vivo.LPAM-GS has great potential in various fields,such as neurology,oncology,and pathology. | Jongbeom Kim Jin Young Kim Seungwan Jeon Jin Woo BAIK Seong Hee Cho Chulhong Kim | 2019 | Light(Science & Applications)2019，8，1： | 6 |
| 2 | Transcriptional up-regulation of disk abalone selenium dependent glutathione peroxidase by H2O2 oxidative stress and Vibrio alginolyticus bacterial infection显示文摘 | Mahanama D Z Wickramaarachchilage A P Chulhong O | 2008 | Fish Shellfish Immun2008，25，： | 1 |
| 3 | Toward in vivo translation of superresolution localization photoacoustic computed tomography using liquid-state dyed droplets显示文摘Super-resolution localization photoacoustic computed tomography has been successfully performed in live animals using an injection of unprecedentedly absorptive liquid-state dyed droplets,which promises a disruptive modality for in vivo neuroimaging. | Wonseok Choi Chulhong Kim | 2019 | Light(Science & Applications)2019，8，1： | 1 |
| 4 | In vivo photoacoustic tomographyof chemicals: high-resolutionfunctional and molecu- lar optical imaging at new depths 显示文摘 | Chulhong Kim | 2010 | NIH Public Access2010，，110： | 1 |
| 5 | Deeply penetrating in vivo photoacoustic imaging using a clinical ultrasound array system显示文摘 | Chulhong Kim Todd N Erpelding Ladislav Jankovic | | 0，，01： | 1 |
| 6 | Characterization of a novel molluscan MyD88 family protein from manila clam, Ruditapes philippinarum显示文摘 | Youngdeuk Lee Ilson Whang Navaneethaiyer Umasuthan Mahanama De Zoysa Chulhong Oh Do-Hyung Kang Cheol Young Choi Choul-Ji Park Jehee Lee | 2011 | Fish and Shellfish Immunology2011，，6： | 1 |
| 7 | 从实验室到市场:光声成像的产业化进程(英文)显示文摘Photoacoustic imaging(PAI) or optoacoustic imaging, the modern application of an ancient physical discovery to biomedical imaging, is without doubt one of the most exciting imaging technologies that has drawn increasing attention from biomedical specialists. In PAI, the rich contrast of optical excitation is seamlessly combined with the high spatial resolution and large penetration depth of ultrasonic detection to produce clear images of optically scattering biological tissues. As a complementary imaging modality that surpasses the territory of traditional microscopic optical imaging, PAI has been explored for numerous biomedical studies, and hence enthusiastically embraced by researchers around the globe who have attested to its unique imaging capabilities, namely the deep penetration and functional sensitivity.Not surprisingly, as the market clearly sees the promise, the commercial production of PAI systems has grown apace with the technological advancements and clinical applications. The adoption of commercial PAI in research and clinical settings has however seen difficulties, majorly due to costs, regulatory blocks,and competition with mainstream technologies. Here, from a practical standpoint, a wide range of commercial PAI systems currently available in the market were introduced, their advantages and disadvantages were analyzed, and the design considerations for targeted applications were emphasized. The key technological, logistical, and clinical issues were also discussed that need to be solved to accelerate the technology translations. By doing so, it is hoped that a clearer picture of the future commercialization of PAI for clinicians, researchers, and industrial entrepreneurs will be presented. | Yasha Saxena Chulhong Kim Yao Junjie | 2017 | 红外与激光工程2017，46，11： | 1 |
| 8 | Performance benchmarks of an array-based hand-held photoacoustic probe adapted from a clinical ultrasound system for non-invasive sentinel lymph node imaging显示文摘 | Kim Chulhong Erpelding Todd N. Jankovic Ladislav Wang Lihong V | 2011 | Philosophical Transactions of the Royal Society A2011，，1955： | 1 |
| 9 | Isolation,Purification,and Enzymatic Characterization of Extracellular Chitosanase from Marine Bacterium Bacillus subtilis CH2显示文摘 | Chulhong O Mahanama DZ Kang DH | | 0，，10： | 1 |
| 10 | Deep learning acceleration of multiscale superresolution localization photoacoustic imaging显示文摘A superresolution imaging approach that localizes very small targets,such as red blood cells or droplets of injected photoacoustic dye,has significantly improved spatial resolution in various biological and medical imaging modalities.However,this superior spatial resolution is achieved by sacrificing temporal resolution because many raw image frames,each containing the localization target,must be superimposed to form a sufficiently sampled high-density superresolution image.Here,we demonstrate a computational strategy based on deep neural networks(DNNs)to reconstruct high-density superresolution images from far fewer raw image frames.The localization strategy can be applied for both 3D label-free localization optical-resolution photoacoustic microscopy(OR-PAM)and 2D labeled localization photoacoustic computed tomography(PACT).For the former,the required number of raw volumetric frames is reduced from tens to fewer than ten.For the latter,the required number of raw 2D frames is reduced by 12 fold.Therefore,our proposed method has simultaneously improved temporal(via the DNN)and spatial(via the localization method)resolutions in both label-free microscopy and labeled tomography.Deep-learning powered localization PA imaging can potentially provide a practical tool in preclinical and clinical studies requiring fast temporal and fine spatial resolutions. | Jongbeom Kim Gyuwon Kim Lei Li Pengfei Zhang Jin Young Kim Yeonggeun Kim Hyung Ham Kkim Lihong V.Wang Seungchul Lee Chulhong Kim | 2022 | Light(Science & Applications)2022，11，6： | 1 |
| 11 | Beyond the acoustic diffraction limit: superresolution localization optoacoustic tomography (LOT)显示文摘Localization optoacoustic tomography provides superresolution imaging capability in 3D beyond the acoustic diffraction limit,which can be crucial for mapping microcirculation in cancers,brain functions,peripheral vascular diseases,etc.Optoacoustic(also referred to as photoacoustic)tomography(OAT)has been gaining popularity for preclinical and clinical imaging during the past couple of decades1.OAT breaks the long-standing shallow imaging depth limitation of conventional optical imaging by forming an image using the optoacoustic(OA)effect.Through advances in ultrasound imaging technologies,OAT provides rich optical contrast while achieving high spatial resolution deep inside living subjects(up to several centimeters).Thanks to these hybrid technologies,the use of preclinical OAT to study cancer physiopathology,neural physiology,drug delivery,vascular diseases,etc.,has spread globally to many laboratories.More importantly,the applications of OAT have been extended to include many clinical trials,such as early diagnosis and treatment monitoring of cancers,imaging of the bowel for diseases,human neuroimaging for diagnosing neurological defects,imaging of peripheral arteries and veins for detecting vascular disease,and intravascular imaging for characterizing plaque. | Chulhong Kim | 2018 | Light(Science & Applications)2018，7，1： | 0 |
| 12 | Super-resolution visible photoactivated atomic force microscopy显示文摘Imaging the intrinsic optical absorption properties of nanomaterials with optical microscopy(OM)is hindered by the optical diffraction limit and intrinsically poor sensitivity.Thus,expensive and destructive electron microscopy(EM)has been commonly used to examine the morphologies of nanostructures.Further,while nanoscale fluorescence OM has become crucial for investigating the morphologies and functions of intracellular specimens,this modality is not suitable for imaging optical absorption and requires the use of possibly undesirable exogenous fluorescent molecules for biological samples.Here we demonstrate super-resolution visible photoactivated atomic force microscopy(pAFM),which can sense intrinsic optical absorption with~8 nm resolution.Thus,the resolution can be improved down to~8 nm.This system can detect not only the first harmonic response,but also the higher harmonic response using the nonlinear effect.The thermoelastic effects induced by pulsed laser irradiation allow us to obtain visible pAFM images of single gold nanospheres,various nanowires,and biological cells,all with nanoscale resolution.Unlike expensive EM,the visible pAFM system can be simply implemented by adding an optical excitation sub-system to a commercial atomic force microscope. | Seunghyun Lee Owoong Kwon Mansik Jeon Jaejung Song Seungjun Shin HyeMi Kim Minguk Jo Taiuk Rim Junsang Doh Sungjee Kim Junwoo Son Yunseok Kim Chulhong Kim | 2017 | Light(Science & Applications)2017，6，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员