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| 1 | Acquired mutation of the tyrosine kinase JAK2 in human myeloproliferative disorders显示文摘 | E Joanna Baxter Linda M Scott Peter J Campbell Clare East Nasios Fourouclas Soheila Swanton George S Vassiliou Anthony J Bench Elaine M Boyd Natasha Curtin Mike A Scott Wendy N Erber Anthony R Green | 2005 | The Lancet . 2005 (9464)2005，，： | 2 |
| 2 | Increased expression of chondroitin sulphate proteoglycans in rat hepatocellular carcinoma tissues显示文摘AIM:To investigate the expression of chondroitin sulphate proteoglycans(CSPGs)in rat liver tissues of hepatocellular carcinoma(HCC).METHODS:Thirty male Sprague Dawley rats were randomly divided into two groups:control group(n=10) and HCC model group(n=20).Rats in the HCC model groups were intragastrically administrated with 0.2%(w/v)N-diethylnitrosamine(DEN)every 5 d for 16 wk,whereas 0.9%(w/v)normal saline was administered to rats in the control group.After 16 wk from the initiation of experiment,all rats were killed and livers were collected and fixed in 4%(w/v)paraformaldehyde.All tissues were embedded in paraffin and sectioned.Histological staining(hematoxylin and eosin and Toluidine blue)was performed to demonstrate the onset of HCC and the content of sulphated glycosaminoglycan(sGAG).Immunohistochemical staining was performed to investigate the expression of chondroitin sulphate(CS)/dermatan sulphate(DS)-GAG,heparan sulphate(HS)-GAG,keratan sulphate(KS)-GAG in liver tissues.Furthermore,expression and distribution of CSPG family members,including aggrecan,versican,biglycan and decorin in liver tissues,were also immunohistochemically determined.RESULTS:After 16 wk administration of DEN,malignant nodules were observed on the surface of livers from the HCC model group,and their hepatic lobule structures appeared largely disrupted under microscope.Toluidine blue staining demonstrated that there was an significant increase in sGAG content in HCC tissues when compared with that in the normal liver tissues from the control group[0.37±0.05 integrated optical density per stained area(IOD/area)and 0.21± 0.01 IOD/area,P<0.05].Immunohistochemical studies demonstrated that this increased sGAG in HCC tissues was induced by an elevated expression of CS/DS(0.28±0.02 IOD/area and 0.18±0.02 IOD/area,P< 0.05)and HS(0.30±0.03 IOD/area and 0.17±0.02 IOD/area,P<0.01)but not KS GAGs in HCC tissues.Further studies thereby were performed to investigate the expression and distribution of several CSPG components in HCC tissues,including aggrecan,versican,biglycan and decorin.Interestingly,there was a distinct distribution pattern for these CSPG components between HCC tissues and the normal tissues.Positive staining of aggrecan,biglycan and decorin was localized in hepatic membrane and/or pericellular matrix in normal liver tissues;however,their expression was mainly observed in the cytoplasm,cell membranes in hepatoma cells and/or pericellular matrix within HCC tissues.Semi-quantitative analysis indicated that there was a higher level of expression of aggrecan(0.43± 0.01 and 0.35±0.03,P<0.05),biglycan(0.32±0.01 and 0.25±0.01,P<0.001)and decorin(0.29±0.01 and 0.26±0.01,P<0.05)in HCC tissues compared with that in the normal liver tissues.Very weak versican positive staining was observed in hepatocytes near central vein in normal liver tissues;however there was an intensive versican distribution in fibrosis septa between the hepatoma nodules.Semi-quantitative analysis indicated that the positive rate of versican in hepatoma tissues from the HCC model group was much higher than that in the control group(33.61%and 21.28%,P <0.05).There was no positive staining in lumican and keratocan,two major KSPGs,in either normal or HCC liver tissues.CONCLUSION:CSPGs play important roles in the onset and progression of HCC,and may provide potential therapeutic targets and clinical biomarkers for this prevalent tumor in humans. | Xiao-Li Jia Si-Yuan Li Shuang-Suo Dang Yan-An Cheng Xin Zhang Wen-Jun Wang Clare E Hughes Bruce Caterson | 2012 | World Journal of Gastroenterology2012，18，30： | 2 |
| 3 | Perinatal and early life risk factors for inflammatory bowel disease显示文摘AIM:To investigate associations between perinatal risk factors and subsequent inflammatory bowel disease (IBD) in children and young adults.METHODS:Record linked abstracts of birth registrations,maternity,day case and inpatient admissions in a defined population of southern England.Investigation of 20 perinatal factors relating to the maternity or the birth:maternal age,Crohn's disease (CD) or ulcerative colitis (UC) in the mother,maternal social class,marital status,smoking in pregnancy,ABO blood group and rhesus status,pre-eclampsia,parity,the infant's presentation at birth,caesarean delivery,forceps delivery,sex,number of babies delivered,gestational age,birthweight,head circumference,breastfeeding and Apgar scores at one and five minutes.RESULTS:Maternity records were present for 180 children who subsequently developed IBD.Univariate analysis showed increased risks of CD among children of mothers with CD (P=0.011,based on two cases of CD in both mother and child) and children of mothers who smoked during pregnancy.Multivariate analysis confirmed increased risks of CD among children of mothers who smoked (odds ratio=2.04,95% CI=1.06-3.92) and for older mothers aged 35+ years (4.81,2.32-9.98).Multivariate analysis showed that there were no significant associations between CD and 17 other perinatal risk factors investigated.It also showed that,for UC,there were no significant associations with the perinatal factors studied.CONCLUSION:This study shows an association between CD in mother and child;and elevated risks of CD in children of older mothers and of mothers who smoked. | Stephen E Roberts Clare J Wotton John G Williams Myfanwy Griffith Michael J Goldacre | 2011 | World Journal of Gastroenterology2011，17，6： | 2 |
| 4 | Requirement of bic/mi- croRNA-155 for normal immune function显示文摘 | Rodriguez A Vigorito E Clare S | 2007 | Science2007，316，5824： | 1 |
| 5 | Halogenated glass system for the protection of structural carbon/carbon composites 显示文摘 | Lobiondo N E Jones L E Clare A G | 1995 | Carbon1995，33，4： | 1 |
| 6 | Laser cladding oflnconel 625wireforcorrosionprotection 显示文摘 | Abioye T E Mccartney D G Clare A T | 2015 | JournalofMaterialsProcess- ing Technology2015，217，： | 1 |
| 7 | Oscillatory fluid flow-induced shear stress decreases osteoclastogenesis through RANKL and OPG signaling显示文摘 | Chi H K Lidan Y Clare E | 2006 | Bone2006，39，5： | 1 |
| 8 | Requirement of bic/mi- croRNA-155 for Normal Immune Function显示文摘 | Rodriguez A Vigorito E Clare S | 2007 | Science2007，316，5824： | 1 |
| 9 | Mackay Voxel-based Morphometric Comparison of Hippocampal and Extra hippocampal Abnormalities in Patients with Left and Right Hippocampal Atrophy 显示文摘 | Simon S Keller Clare E | 2002 | Neurolmage2002，16，： | 1 |
| 10 | Food allergen pro-rein families and their structural characteristics and application in component - resolved diagnosis: new data from the EuroPrevall project 显示文摘 | Hoffmann - sommergruber K Clare Mills E N | 2009 | Analytical and bioanalytical chemistry2009，395，1： | 1 |
| 11 | Bioactive milk peptides:a prospectus显示文摘 | CLARE D A SWAISGOOD H E | 2000 | Journal of Dairy Science2000，83，6： | 1 |
| 12 | Plant food allerges--structural and functional aspects of allergenicity 显示文摘 | Breiteneder H Clare mills E N | 2005 | Biotechnology ad- vances2005，23，6： | 1 |
| 13 | A parametric study oflncone1625 wire laser deposition显示文摘 | Abioye T E Folkes J Clare A T | 2013 | Journal of Materials Processing Technolo- gy2013，213，12： | 1 |
| 14 | Bioactive milk peptides:Aprospectus显示文摘 | Clare D A Swaisgood H E | 2000 | Journal of Dairy Science2000，83，6： | 1 |
| 15 | Requirement of bic/ microRNA-155 for normal immune function显示文摘 | Rodriguez A Vigorito E Clare S | 2007 | Science2007，316，5824： | 1 |
| 16 | Requirement of bic/microRNA-155 for normal immune function显示文摘 | Rodriguze A Vigorito E Clare S | 2007 | Science2007，316，5824： | 1 |
| 17 | Nano-engineering of 5-fluorouracil-loaded magnetoliposomes for combined hyperthermia and chemotherapy against colon cancer 显示文摘 | Clares B Biedma-Ortiz RA SOez-FernOndez E | 2013 | Eur J Pharm Biopharm2013，85，3： | 1 |
| 18 | Laser Clad- ding of Inconel 625 Wire for Corrosion Protection显示文摘 | ABIOYE T E MCCARTNEY D G CLARE A T | 2015 | Jour- nal of Materials Processing Technology2015，217，： | 1 |
| 19 | Requirement of bic/microRNA-155 for normal immune function 显示文摘 | Rodriguez A Vigorito E Clare S | 2007 | Science2007，316，： | 1 |
| 20 | Requirement of bic/microRNh-155 for normal immune function显示文摘 | Vigorito E Clare S | 2007 | Science2007，316，5824： | 1 |
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