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| 1 | Immune response-related genes associated to blocking midgut dengue virus infection in Aedes aegypti strains that differ in susceptibility显示文摘Aedes (Stegomyia) aegypti, the principal global vector of dengue viruses, has difTerences in its susceptibility to dengue virus infection. We compared the global expres? sion of genes in the midguts of Colombian Ae. aegypti dengue-susceptible (Cali-S) and dengue-refractory (Cali-MIB) field derived strains after ingesting either a sugarmeal, a bloodmeal, or a bloodmeal containing dengue virus serotype 2 (DENV-2). Microarraybased transcriptome analysis among treatments indicated a total of 4725 transcripts with differential expression between the two strains. Eleven genes were selected from different functional groups based on their significant up or down expression levels as well as reports in the literature suggesting they are associated with dengue virus elimination. We measured mRNA abundance of these 11 genes at 0, 8, 24, and 36 h postinfection using quantitative real time PCR (qPCR) to confirm the microarray results and assess any temporal patterns. Four genes were selected (Gram-negative binding protein—GNBP [AAEL009176], Niemann Pick Type-C2一NPC2 [AAEL015136], Keratinocyte lectin [AAEL009842], and Cathepsin-b [AAEL007585]) for knockdown experiments using RNA interference (RNAi) methodology to determine the phenotype (DENV-2 susceptible or refractory). Silencing GNBP、Cathepsin-b and Keratinocyte lectin reduced the percentage of mosquitoes with disseminated virus in the Cali-S strain to 8%, 20%, and 12% respectively compared with 96% in the controls. Sile ncing of NPC2 increased the percentage of mosquitos with disseminated virus infections in Cali-MIB to 66% compared with 35% in the controls. This study provides in sight into genes that may contribute to the Cali-S susceptible and Cali-MIB refractory phenotypes in 昇匕 aegypti. | Paola A. Caicedo Idalba Mildred Serrato Shuzhen Sim George Dimopoulos Heather Coatsworth Carl Lowenberger Clara B. Ocampo | 2019 | Insect Science2019，26，4： | 2 |
| 2 | The structure and coherence of competence from childhood through adolescence 显示文摘 | Masters AS Coatsworth JD Neemann J | 1995 | Child Development1995，66，： | 1 |
| 3 | The structure and coherence of competence from childhood through adolescence显示文摘 | Masten A S Coatsworth J D Neemann J | 1995 | Child Development1995，66，： | 1 |
| 4 | The development of competence in favorable and unfavorable environments: Lessons from research on successful children显示文摘 | MASTEN A S COATSWORTH J D | 1998 | Psychol1998，53，2： | 1 |
| 5 | The structure and coherence of competence from childhood through adolescence显示文摘 | MASTEN A S COATSWORTH J D NEEMANN J | 1995 | Child development1995，66，6： | 1 |
| 6 | A case of carbapenemase producing Klebsiella pneumoniae in Australia 显示文摘 | Coatsworth NR Huntington PG Hardiman RP | 2012 | Pathology2012，44，1： | 1 |
| 7 | The development of competence in favorable and unfavorable environments:Lessons from research on successful children显示文摘 | Masten A S Coatsworth J D | 1998 | American Psychologist1998，53，2： | 1 |
| 8 | Enhancing the self - esteem of youth swimmers through coach training: Gender and age effects 显示文摘 | Coatsworth J D Conroy D E | 2006 | Psychology of sport and exercise2006，7，2： | 1 |
| 9 | The development of competence in favorable and unfavorable environments : lessons from research on successful children 显示文摘 | Masten AS Coatsworth JD | 1998 | Am Psychologist1998，53，： | 1 |
| 10 | Studies of nitrate reductase in marine phytoplankton显示文摘 | Eppley R W Coatsworth J L Solórzano L | 1969 | Limnol Oceanogr1969，14，： | 1 |
| 11 | The Development of Competence in Favorable and Unfavorable Environments:Lessons from Research on Successful Children 显示文摘 | Masten A S Coatsworth J D | 1998 | American Psychologist1998，，19： | 1 |
| 12 | The structure and coherence of competence from childhood through adolescence显示文摘 | Mast AS Coatsworth JD Neemami J | | 0，，： | 1 |
| 13 | The structure and coherence of competence from childhood through adolescence 显示文摘 | MASTEN A S COATSWORTH J D NEEMANN J | 1995 | Child Development1995，66，： | 1 |
| 14 | The structure and coherence of competence from childhood through adolescence 显示文摘 | Masten A S Coatsworth J D Neemann J | 1995 | Child Development1995，，66： | 1 |
| 15 | The structure and coherence of competence from childhood through adolescence显示文摘 | Masten A S Coatsworth J D Neemann J | 1995 | Child Development1995，，66： | 1 |
| 16 | The development of competence in favorable and unfavorable environments:Lessons form research on successful children显示文摘 | Masten A Coatsworth D | | 0，，： | 1 |
| 17 | Nitrate and nitrite uptake by Ditylum brightwellii Kinetics and mechanisms 显示文摘 | Eppley R W Coatsworth J L | 1968 | Journal of Phycology1968，4，： | 1 |
| 18 | Enhancing the self-esteem of youth swimmers through coach training: gender and age effects显示文摘 | Coatsworth J Conroy D E | 2006 | Psych SportExerc2006，7，： | 1 |
| 19 | 查看详情显示文摘 | Duncan R Coatsworth J.K Burtles S | | 0，，： | 1 |
| 20 | Studies of nitrate reductase in marine phytoplankton 显示文摘 | Eppley R W Coatsworth J L Solórzano L | 1969 | Limnology & Oceanography1969，14，： | 1 |
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