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| 1 | Specificity Requirements for Human Telomere Protein Interaction with Telomerase Holoenzyme显示文摘 | Sexton AN Youmans DT Col ins K | | 0，，41： | 1 |
| 2 | Quantifying nuclear p65 as a parameter for NF-κB activation:Correlation between ImageStream cytometry,microscopy,and Western blot显示文摘 | Maguire O Col ins C O'Loughlin K | | 0，，06： | 1 |
| 3 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 4 | Retinoic acid/alpha-inter- feron combination inhibits growth and promotes apoptosis in mantle cell lymphoma through Akt-dependent modulation of critical targets 显示文摘 | Dal Col J Mastorci K Fa6 DA | 2012 | Cancer Res2012，72，7： | 1 |
| 5 | In vitro degradation of silk fibroin显示文摘 | Horan RL Antle K Col ette AL | | 0，，17： | 1 |
| 6 | Neural responses to facial expressions of disgust but not fear are modulated by washing symptoms in OCD 显示文摘 | LAWRENCE N S AN S K MATAIX- COLS D | 2007 | Biol Psychiatry2007，61，9： | 1 |
| 7 | The evaluation of coagulation profiles in calves with suspected septic shock 显示文摘 | Irmak K Sen I Col R | 2006 | Vet Res Commun2006，30，5： | 1 |
| 8 | In vitro degradation of silk fi- broln 显示文摘 | Horan RL Antle K Col|ette AL | 2005 | Biomaterials2005，26，17： | 1 |
| 9 | Elasticity and viscoelasticity of embolization microspheres显示文摘 | Hidaka K Moine L Col in G | | 0，，08： | 1 |
| 10 | Detection of aflatoxin M1 levels by ELISA in white-brined Urfa cheese con- sumed in Turkey显示文摘 | Kav K Col R Kaan Tekinsen K | 2011 | Food Control2011，22，12： | 1 |
| 11 | Barriers to Providing Osteoporosis care in Skilled Nursing Facilities:Perceptions of Medical Directors and Directors of Nursing显示文摘 | Col(o)n-Emeric C S Casebeer L Saag K | 2005 | J Am Med Dir Assoc2005，6，3： | 1 |
| 12 | The learning curve for sentinel node biopsy in breast cancer:practical considerations显示文摘 | Orr R K Hoehn J L Col N F | 1999 | Arch Surg1999，134，7： | 1 |
| 13 | A novel role for vascular endothelial growth factor as an autocrine survival factor for embryonic stem cel s during hypoxia显示文摘 | Brusselmans K Bono F Col en D | | 0，，05： | 1 |
| 14 | Prospective randomized clinical trial of inpatient cervical ripening with stepwise oral misoprostol vs vaginal misoprostol显示文摘 | Colón I Clawson K Hunter K | 2005 | Am J Obstet Gynecol2005，192，： | 1 |
| 15 | OCT4 expression in human non-small cell lung cancer: implications for therapeutic intervention 显示文摘 | Col K Mat G Ral S | 2009 | Interact Cardiovase Thorac Surg2009，8，4： | 1 |
| 16 | Conduction velocity is regulated by sodium channel inactivation in unmyelinated axons innervating the rat cranial meninges 显示文摘 | Col DR Messlinger K Carr RW | 2008 | J Physiol2008，586，： | 1 |
| 17 | Abnormal rotational knee motion during running after anterior cruciate ligament reconstruction显示文摘 | Tashman S Col on D Anderson K | | 0，，04： | 1 |
| 18 | Gastric Tonometry as a Prognostic Index of Mortality in Sepsis显示文摘 | Col K K Upadhyay V S M Brig V P S et aI | 2007 | MJAFI2007，63，： | 1 |
| 19 | COL6A1 gene and Ironman triathlon performance显示文摘 | O'Connel K Posthumus M Col ins M | | 0，，11： | 1 |
| 20 | Orocutaneous manifestations as markers of disease progression in HIV infection in indian setting显示文摘 | Brig Y K Sharma Col MPS Sawhney Le Col D S Bhakuni | 2004 | Medical J Armed Forces India2004，60，： | 1 |
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