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1篇 您的检索式:作者名="Colin J.R.Sheppard"
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1Advanced optical microscopy methods for in vivo imaging of sub-cellular structures in thick biological tissuesl显示文摘Optical microscopy has become an indispensable tool for visualizing sub-cellular structures andbiological processes.However,scattering in biological tissues is a major obstacle that preventshigh-resolution images from being obtained from deep regions of tissue.We review commontechniques,such as multiphoton microscopy(MPM)and optical coherence microscopy(OCM),for diffraction limited imaging beyond an imaging depth of 0.5 mm.Novel implementations havebeen emerging in recent years giving higher imaging speed,deeper penetration,and better imagequality.Focal modulation microscopy(FMM)is a novel method that combines confocal spatialfltering with focal modulation to reject out-of-focus background.FMM has demonstrated animaging depth comparable to those of MPM and OCM,near-real-time image acquisition,and thecapability for multiple contrast mechanisms.Nanguang Chen Shakil Rehman Colin J.R.Sheppard 2014Journal of Innovative Optical Health Sciences2014,7,5:0
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