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| 1 | Tetracycline-inducible protein expression in pancreatic cancer cells: Effects of CapG overexpression显示文摘AIM: To establish stable tetracycline-inducible pancreatic cancer cell lines. METHODS: Suit-2, MiaPaca-2, and Panc-1 cells were transfected with a second generation reverse tetracycline-controlled transactivator protein (rtTA2S-M2), under the control of either a cytomegalovirus (CMV) or a chicken β-actin promoter, and the resulting clones were characterised.RESULTS: Use of the chicken (β-actin) promoter proved superior for both the production and maintenance of doxycycline-inducible cell lines. The system proved versatile, enabling transient inducible expression of a variety of genes, including GST-P, CYP2E1, S100A6, and the actin capping protein, CapG. To determine the physiological utility of this system in pancreatic cancer cells, stable inducible CapG expressors were established. Overexpressed CapG was localised to the cytoplasm and the nuclear membrane, but was not observed in the nucleus. High CapG levels were associated with enhanced motility, but not with changes to the cell cycle, or cellular proliferation. In CapG-overexpressing cells, the levels and phosphorylation status of other actin-moduating proteins (Cofilin and Ezrin/Radixin) were not altered. However, preliminary analyses suggest that the levels of other cellular proteins, such as ornithine aminotransferase and enolase, are altered upon CapG induction. CONCLUSION: We have generated pancreatic-cancer derived cell lines in which gene expression is fully controllable. | Sarah Tonack Sabina Patel Mehdi Jalali Taoufik Nedjadi Rosalind E Jenkins Christopher Goldring John Neoptolemos Eithne Costello | 2011 | World Journal of Gastroenterology2011，17，15： | 4 |
| 2 | Comparative Effectiveness for Survival and Renal Function of Partial and Radical Nephrectomy for Localized Renal Tumors: A Systematic Review and Meta-Analysis显示文摘 | Simon P. Kim R. Houston Thompson Stephen A. Boorjian Christopher J. Weight Leona C. Han M. Hassan Murad Nathan D. Shippee Patricia J. Erwin Brian A. Costello George K. Chow Bradley C. Leibovich | 2012 | The Journal of Urology2012，，： | 2 |
| 3 | Enforcing Emissions Trading when Emissions Permits are Bankable显示文摘 | John K. Stranlund Christopher Costello Carlos A. Chávez | 2005 | Journal of Regulatory Economics2005，，2： | 2 |
| 4 | Unintended biological invasions: Does risk vary by trading partner? 显示文摘 | Costello Christopher Springbom Michael McAusland Carol et al | 2007 | Journal of Environmental Economics and Management2007，54，3： | 1 |
| 5 | Potential use of circulating endothelial cells as a biomarker of renal cell carcinoma显示文摘 | Kevin V.S. Tan Benjamin Namdarian Anthony J. Costello Christopher M. Hovens | 2011 | Urologic Oncology: Seminars and Original Investigations2011，，3： | 1 |
| 6 | Snail expression is an independent predictor of tumor recurrence in superficial bladder cancers显示文摘 | Franck Bruyere Benjamin Namdarian Niall M. Corcoran John Pedersen Jeremy Ockrim Bryan B. Voelzke Uttam Mete Anthony J. Costello Christopher M. Hovens | 2010 | Urologic Oncology: Seminars and Original Investigations2010，，： | 1 |
| 7 | Acute Abdomen显示文摘 | Stephan Zangos Scott D. Steenburg K. Dan Phillips J. Matthias Kerl Shaun A. Nguyen Christopher Herzog U. Joseph Schoepf Thomas J. Vogl Phillip Costello | 2007 | Academic Radiology2007，，1： | 1 |
| 8 | Snail expression is an independent predictor of tumor recurrence in superficial bladder cancers显示文摘 | Franck Bruyere Benjamin Namdarian Niall M. Corcoran John Pedersen Jeremy Ockrim Bryan B. Voelzke Uttam Mete Anthony J. Costello Christopher M. Hovens | 2010 | Urologic Oncology: Seminars and Original Investigations2010，，6： | 1 |
| 9 | CT detection of myocardial blood volume deficits: Dual-energy CT compared with single-energy CT spectra显示文摘 | Elisabeth Arnoldi Yeong Shyan Lee Balazs Ruzsics Markus Weininger J. Reid Spears Christopher P. Rowley Salvatore A. Chiaramida Philip Costello Maximilian F. Reiser U. Joseph Schoepf | 2011 | Journal of Cardiovascular Computed Tomography2011，，6： | 1 |
| 10 | Using portfolio theory to assess tradeoffs between return from natural capital and social equity across space显示文摘 | Benjamin S. Halpern Crow White Sarah E. Lester Christopher Costello Steven D. Gaines | 2011 | Biological Conservation2011，，5： | 1 |
| 11 | Science in support of ecosystem-based management for the US West Coast and beyond显示文摘 | Sarah E. Lester Karen L. McLeod Heather Tallis Mary Ruckelshaus Benjamin S. Halpern Phillip S. Levin Francisco P. Chavez Caroline Pomeroy Bonnie J. McCay Christopher Costello Steven D. Gaines Amber J. Mace John A. Barth David L. Fluharty Julia K. Parrish | 2009 | Biological Conservation2009，，3： | 1 |
| 12 | On Trade, Land -use and Biodiversity显示文摘 | Stephen Polasky Christopher Costello and Carol McAusland | 2004 | Journal of Environment Economics and Management2004，，48： | 1 |
| 13 | Comparative Effectiveness for Survival and Renal Function of Partial and Radical Nephrectomy for Localized Renal Tumors: A Systematic Review and Meta-Analysis显示文摘 | Simon P. Kim R. Houston Thompson Stephen A. Boorjian Christopher J. Weight Leona C. Han M. Hassan Murad Nathan D. Shippee Patricia J. Erwin Brian A. Costello George K. Chow Bradley C. Leibovich | 2012 | The Journal of Urology2012，，1： | 1 |
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