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| 1 | Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery显示文摘AIM: To investigate the impact of minor abdominal surgery on the caecal microbial population and on markers of gut inflammation.METHODS: Four week old piglets were randomly allocated to a no-surgery 'control' group(n = 6) or a 'transection surgery' group(n = 5).During the transection surgery procedure, a conventional midline incision of the lower abdominal wall was made and the small intestine was transected at a site 225 cm proximal to the ileocaecal valve, a 2 cm segment was removed and the intestine was re-anastomosed.Piglets received a polymeric infant formula diet throughout the study period and were sacrificed at two weeks post-surgery.Clinical outcomes including weight, stool consistency and presence of stool fat globules were monitored.High throughput DNA sequencing of colonic content was used to detect surgery-relateddisturbances in microbial composition at phylum, family and genus level.Diversity and richness estimates were calculated for the control and minor surgery groups.As disturbances in the gut microbial community are linked to inflammation we compared the gene expression of key inflammatory cytokines(TNF, IL1 B, IL18, IL12, IL8, IL6 and IL10) in ileum, terminal ileum and colon mucosal extracts obtained from control and abdominal surgery groups at two weeks post-surgery.RESULTS: Changes in the relative abundance of bacterial species at family and genus level were confined to bacterial members of the Proteobacteria and Bacteroidetes phyla.Family level compositional shifts included a reduction in the relative abundance of Enterobacteriaceae(22.95 ± 5.27 vs 2.07 ± 0.72, P < 0.01), Bacteroidaceae(2.54 ± 0.56 vs 0.86 ± 0.43, P < 0.05) and Rhodospirillaceae(0.40 ± 0.14 vs 0.00 ± 0.00, P < 0.05) following transection surgery.Similarly, at the genus level, changes associated with transection surgery were restricted to members of the Proteobacteria and Bacteroidetes phyla and included decreased relative abundance of Enterobacteriaceae(29.20 ± 6.74 vs 2.88 ± 1.08, P < 0.01), Alistipes(4.82 ± 1.73 vs 0.18 ± 0.13, P < 0.05) and Thalassospira(0.53 ± 0.19 vs 0.00 ± 0.00, P < 0.05).Surgeryassociated microbial dysbiosis was accompanied by increased gene expression of markers of inflammation.Within the ileum IL6 expression was decreased(4.46 ± 1.60 vs 0.24 ± 0.06, P < 0.05) following transection surgery.In the terminal ileum, gene expression of TNF was decreased(1.51 ± 0.13 vs 0.80 ± 0.16, P < 0.01) and IL18(1.21 ± 0.18 vs 2.13 ± 0.24, P < 0.01), IL12(1.04 ± 0.16 vs 1.82 ± 0.32, P < 0.05) and IL10(1.04 ± 0.06 vs 1.43 ± 0.09, P < 0.01) gene expression increased following transection surgery.Within the colon, IL12(0.72 ± 0.13 vs 1.78 ± 0.28, P < 0.01) and IL10(0.98 ± 0.02 vs 1.95 ± 0.14, P < 0.01) gene expression were increased following transection surgery.CONCLUSION: This study suggests that minor abdominal surgery in infants, results in long-term alteration of the colonic microbial composition and persistent gastrointestinal inflammation. | Susan Lapthorne Julie E Bines Fiona Fouhy Nicole L Dellios Guineva Wilson Sarah L Thomas Michelle Scurr Catherine Stanton Paul D Cotter Prue M Pereira-Fantini | 2015 | World Journal of Gastroenterology2015，21，14： | 3 |
| 2 | Incidence of atrial fibrillation detected by implantable loop recorders in unexplained stroke显示文摘 | Cotter PE Martin PJ Ring L | 2013 | Neurology2013，80，： | 1 |
| 3 | The dual endothelin receptor antagonist tezosentan acutely improves hemodynamic parameters in patients with advanced heart failure显示文摘 | Schalcher C Cotter G Reisin L | 2001 | Am Heart J2001，142，2： | 1 |
| 4 | MCMC methods for functions:modifying old algorithms to make them faster显示文摘 | COTTER S L ROBERTS G O STUART A M | 2013 | Statistical Science2013，28，3： | 1 |
| 5 | Diffferential resis - tance to Staphylococcus aureus challenge in major histocompatibility (B) complex congenic lines显示文摘 | Cotter PF Robert L Taylor JR | 1992 | Poultry Science1992，71，： | 1 |
| 6 | Effects of Feeding Blends of Grains Naturally Contaminated with Fusarium Mycotoxins on Production and Metabolism in Broiler 显示文摘 | Swamy H V L N Smith T K Cotter P F | 2002 | Poult Sci2002，81，7： | 1 |
| 7 | Bacteriocins : Bi- ological tools for biopreservation and shelf-life extension 显示文摘 | Deegan L H Cotter P D Hill C | 2006 | International Dairy Journal2006，16，9： | 1 |
| 8 | Bacteriocins: biological tools for bio- preservation and shelf- life extension 显示文摘 | Deegan L H Cotter P D Hill C | 2006 | International Dairy Journal2006，16，9： | 1 |
| 9 | Dlgl-PTEN interac- tion regulates myelin thickness to prevent damaging peri- pheral nerve overmyelination 显示文摘 | Cotter L Ozelik M Jacob C | 2010 | Science2010，328，5984： | 1 |
| 10 | The Virion Host Shutoff Protein of Herpes Simplex Virus 1 Blocks the Replication-independent Activation of NF-kB in Dendritic Cells in the Absence of Type 1 Interferon Signaling显示文摘 | COTTER C R KIM W K NQUYEN M L | 2011 | J Virol2011，85，12： | 1 |
| 11 | Bacteriocins:biological tools for bio-preservation and sheff-lifeextenaion显示文摘 | DEEGAN L H COTTER P D HILL C | 2006 | International Dairy Journal2006，16，： | 1 |
| 12 | Bacteriocins: biologicaltools for bio-preservation and shelf-life extension显示文摘 | DEEGAN L H COTTER P D HILL C | 2006 | InternationalDairy Journal2006，16，9： | 1 |
| 13 | Influence of process parameters on growth of Clostridium ljungdahlii and Clostridium autoethanogenum on synthesis gas 显示文摘 | Cotter J L Chinn M S Grunden A M | 2009 | Enzyme and Microbial Technology2009，44，5： | 1 |
| 14 | Comparison of energy and protein intakes of older people consuming a texture modified diet with a normal hospital diet显示文摘 | Wright L Cotter D Hickson M | | 0，，： | 1 |
| 15 | Bacteriocins : Biolog- ical tools for bio-preservation and shelf-life extension 显示文摘 | Deegan L H Cotter P D Hill C | 2006 | International Dairy Journal2006，16，9： | 1 |
| 16 | Ethanol and ace- tate production by Clostridium ljungdahlii and Clostridium autoethanogenum using resting cells 显示文摘 | Cotter J L Chinn M S Grunden A M | 2009 | Bioprocess and Biosystems Engineering2009，32，3： | 1 |
| 17 | Ethanol and ac- etate production by Clostridium ljungdahlii and Clostridium autoethanogenum using resting cells显示文摘 | Cotter J L Chinn M S Grunden A M | 2009 | Bioprocess Biosyst Eng2009，32，： | 1 |
| 18 | Influence of process parameters on growth of Clostridium ljungdahlii and Clostridium au toethanogenum on synthesis gas 显示文摘 | JACQUELINE L COTTER CHINN MARI S GRUNDEN AMY M | 2009 | Enzyme and Microbial Technology2009，44，5： | 1 |
| 19 | The dual endothelin receptor antagonist tezosentan acutely improves hemedynamic parameters in patients with advanced heart failure 显示文摘 | Schalcher C Cotter G Reisin L | 2001 | Am Heart J2001，142，2： | 1 |
| 20 | Bacteriocins: biological tools for bio-preservation and shelf-life extension 显示文摘 | DEEGAN L H COTTER P D HILL C | 2006 | International Dairy Journal2006，6，9： | 1 |
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